BioSample

Maize Full length cDNA library (3530 library) created by Invitrogen from multiple tissues; Organ: silks, husks, ears, pollen, shoot tips, leaf, root tips, whole seed, embryo. This is a Gateway compatible vector, permitting clone movement to new vector backbones for expression in diverse host cells using recombination rather than restriction enzymes. poly(A)+ mRNA was prepared by Invitrogen, and equimolar amounts of RNA from each of the 12 tissue samples were mixed together for selection of mRNA with a 5' cap. After synthesis of cDNA, a normalization step was conducted against the mixture of RNA sources. Tissues prepared: 1. just emerging silks; 2. inner husks from ears of sample #1; 3. 20 dap aleurone; 4. immature tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day old seedlings; all leaves with an expanded or partially expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 cm long root tips from 15 day old seedlings; 10. 10 dap whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap endosperm and embryo. All of the sequenced clones in Maize Full-length cDNA Project will be archived at the University of Arizona. Clones, high density filters and amplified library can be ordered from the University of Arizona (http://www.genome.arizona.edu/orders/).