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This cDNA library was constructed from polyA+ enriched mRNA from etiolated hypocotyls 48 h post-inoculation with Phytophthora sojae zoospores. Complementary DNA was synthesized from mRNA using an XhoI-poly(dT) linker-primer. EcoRI adapters were ligated to the blunt-ended cDNA fragments and the products were digested with XhoI for directional cloning into lambda ZAP Express vector. This lambda library was amplified once using E. coli host strain XL1 Blue MRF'. Inserts were then subcloned by mass excision using ExAssist helper phage for conversion into phagemid vector pBK-CMV in E. coli host strain XLOLR. Sequenced using T3 primer: 5' ATT AAC CCT CAC TAA AGG GA 3'.
Nucleotide
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