Figure 1. Overview of the procedure used to differentiate cells towards hepatocytes.

Figure 1Overview of the procedure used to differentiate cells towards hepatocytes.

A. Pluripotent cells are cultured on an E-Cad-Fc substrate before being transferred to Matrigel. Cells are then sequentially exposed to factors with known roles in controlling hepatocyte formation during embryogenesis. Images on right show phase contrast micrographs of cells at each stage of the differentiation procedure. B. Micrograph showing the distribution of pluripotent stem cell colonies on E-cad-Fc immediately before plating for differentiation. C. Higher resolution micrograph of a single colony of pluripotent stem cells immediately before plating for differentiation. D. Micrograph showing distribution of pluripotent stem cells after plating on Matrigel and immediately before initiating differentiation. Scale bar = 50 μm.

From: Protocol for directed differentiation of human pluripotent stem cells toward a hepatocyte fate

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StemBook [Internet].
Cambridge (MA): Harvard Stem Cell Institute; 2008-.
Copyright: © 2012 Uri Ben-Davi and Nissim Benvenisty.

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