NCBI Bookshelf. A service of the National Library of Medicine, National Institutes of Health.
StemBook is an open access collection of invited, original, peer-reviewed chapters covering a range of topics related to stem cell biology written by top researchers in the field at the Harvard Stem Cell Institute and worldwide. StemBook is aimed at stem cell and non-specialist researchers.
In addition to the contributions of the editorial board and the stem cell research community, the project is being done in collaboration with several other enterprises including Harvard’s Initiative in Innovative Computing. The Initiative in Innovative Computing created the Scientific Collaboration Framework (SCF), the extensible software infrastructure used for the project. SCF and the StemBook project were funded, in part, by a generous grant from an anonymous foundation and also appreciates the input of WormBase's Textpresso team.
Contents
- About StemBook
- Cellular and nuclear reprogramming
- Ectoderm specification and differentiation
- Endoderm specification and differentiation
- Epigenetics
- Genomics and proteomics
- Germ cell and somatic stem cell biology in reproduction
- Regulation of spermatogonia
- piRNA function in germline development
- The role of microRNAs in germline differentiation
- Germline stem cell niches
- Uterine stem cells
- Modeling germ cell differentiation
- Lineage analysis of stem cells
- Somatic stem cells of the ovary and their relationship to human ovarian cancers
- Sex differentiation in mouse and man and subsequent development of the female reproductive organs
- C. elegans germline stem cells and their niche
- Manufacturing
- Mesoderm specification and differentiation
- Niche biology, homing, and migration
- Renewal
- Stem cell immunology
- Therapeutic prospects
- Tissue engineering
- Combining stem cells and biomaterial scaffolds for constructing tissues and cell delivery
- Autologous Approaches to Tissue Engineering
- Flow perfusion culture of mesenchymal stem cells for bone tissue engineering
- Engineering microenvironments to control stem cell fate and function
- The role of bone marrow-derived stem cells in lung regeneration and repair
- Mechanical control of stem cell differentiation
- Skin tissue engineering
- Molecular imaging of stem cells
- Protocols
- Pluripotent Cells
- Embryoid body formation from human pluripotent stem cells in chemically defined E8 media
- Genome editing in human pluripotent stem cells
- Splitting hPSCs with Dispase
- Splitting hESC/hiPSC lines with EDTA in feeder free conditions
- Splitting hESC/hiPSC lines on MEF using Accutase
- General Spinfection Protocol
- Assessment of human pluripotent stem cells with PluriTest
- Conditioning pluripotent stem cell media with mouse embryonic fibroblasts (MEF-CM)
- Feeder-independent culture protocol – EDTA splitting
- Feeder dependent (MEF) culture protocol – collagenase passaging
- iPSC derivation from fibroblast in chemically defined medium
- Cryopreservation of human pluripotent stem cells in defined conditions
- Formation of embryoid bodies from Matrigel dots protocol
- Teratoma formation: A tool for monitoring pluripotency in stem cell research
- Basic pluripotent stem cell culture protocols
- Reprogramming
- iPSC Reprogramming from Human Peripheral Blood Using Sendai Virus Mediated Gene Transfer
- Protocol for making retroviral reprogramming factors
- Hematopoietic differentiation
- Robust generation of hepatocyte-like cells from human embryonic stem cell populations
- Monolayer endoderm differentiation from human ESCs
- Blood - SeV derived fibroblast generated iPSCs
- Protocol for directed differentiation of human pluripotent stem cells toward a hepatocyte fate
- Ectoderm
- Endoderm
- Mesoderm
- Cell Imaging
- Pluripotent Cells
Edited by The Stem Cell Research Community