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Antiretroviral Therapy for HIV Infection in Infants and Children: Towards Universal Access: Recommendations for a Public Health Approach: 2010 Revision. Geneva: World Health Organization; 2010.

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Antiretroviral Therapy for HIV Infection in Infants and Children: Towards Universal Access: Recommendations for a Public Health Approach: 2010 Revision.

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ANNEX ISUMMARY OF WHO RECOMMENDATIONS ON LABORATORY INVESTIGATIONS FOR CLINICAL CARE BY LEVEL OF HEALTH CARE FACILITY

Laboratory tests recommended by level of health care facility for providing HIV-related clinical care. These tables are adapted from WHO, available at http://www.who.int/hiv/amds/WHOLabRecommendationBylevelFinal.pdf

Laboratory tests for diagnosis and monitoring 1Primary care level 2District level 3Regional or referral level 4National level 5
Send out 6Perform on-siteSend out 6Perform on-siteSend out 6Perform on-sitePerform on-site
HIV antibody testing
Lab ELISA
Rapid point of care 1
Rapid point of care 2
Rapid point of care 3
HIV virological diagnostic testing
RNA
DNA
Up24 Ag
HIV viral load measurement
Haemoglobin
Haemoglobinometer such as HemoCue
WHO colour scale
Full blood count and differential
CD4
Absolute count
%
HIV resistance testing78
Pregnancy testing
Urine rapid test ? 9? 9
Liver function tests (transaminases and bilirubin)
Glucose
Blood dipstick
Urine dipstick
Blood
Serum electrolytes?
Renal function tests (creatinine/urea)
Urine protein dipstick
Lipids
Amylase
Lactate
Oxygen saturation – pulse oximetry
Blood gases Ph
1

These are the generic recommendations and it is recognized that exceptions apply (e.g., some health centers will send out some tests). Where appropriate these tests may used for public health surveillance and quality assurance activities.

2

Primary health care services are those providing first point of contact with the health care system.

3

District level is defined as hospital at the first referral level that is responsible for a district of a defined geographical area containing a defined population and governed by a politico-administrative organization such as a district health management team.

4

In some smaller countries there may not be regional laboratories.

5

Including the national reference laboratory or public health laboratory, which is usually responsible for quality assurance and surveillance activities.

6

“Send out” refers to not having the testing capability on site and samples or patients are sent to another site for the actual test to be performed.

7

HIV DR testing is only recommended as part of national efforts for surveillance and monitoring as outlined in WHO HIV DR strategy and is usually done by sending out to an accredited international lab.

8

May be done at national level or sent to accredited lab.

9

May need to reconsider recommendation at primary care level.

WHO ART guidelines recommend in context of efavirenz initiation.

Pregnancy test not recommended for routine pregnancy care at the primary level. Most women seek antenatal care when pregnancy is “obvious” and the pregnancy test does not add additional information.

Diagnostic tests for treatable co-infections and major HIV-related opportunistic diseases

Laboratory tests for diagnosis and monitoring 1Primary care level 2District level 3Regional or referral level 4National level 5
Send out 6Perform on-siteSend out 6Perform on-siteSend out 6Perform on-sitePerform on-site
Tuberculosis
Microscopy
 Light7
 Fluorescence
Culture
 Solid medium? 7
 Liquid medium
Drug susceptibility tes 8
 First-line
 Second-line
Diagnosis of malaria
Rapid test for malaria
Microscopy for malaria (thick/thin)
Cerebrospinal fluid (CSF)
Basic CSF microscopy including India ink and Gram stain and Ziehl-Neelsen
CSF culture
CSF glucose
Cryptococcal antigen (serum or CSF)
STI diagnostic tests
Microscopy (Gram & wet)
Gonorrhea
Chlamidya
Syphilis rapid diagnostic test
Syphilis serological (RPR, FTA and TPHA)
Hepatitis B
Hepatitis C1210
PCP
Blood culture
Radiography

References for Summary of WHO recommendations on laboratory investigations for clinical care by level of health care facility are available at http://www​.who.int/hiv​/amds/WHOLabRecommendation-BylevelFinal.pdf

1

These are the generic recommendations and it is recognized that exceptions apply (e.g., some health centers will send out some tests). Where appropriate these tests may used for public health surveillance and quality assurance activities.

2

Primary health care services are those providing first point of contact with the health care system.

3

District level is defined as hospital at the first referral level that is responsible for a district of a defined geographical area containing a defined population and governed by a politico-administrative organization such as a district health management team.

4

In some smaller countries there may not be regional laboratories.

5

Including the national reference laboratory or public health laboratory, which is usually responsible for quality assurance and surveillance activities.

7

At some health centres depending on specimen load.

8

Given complexity of usng DSTs may need adaptation.

9

Provision guided by Hepatitis C prevalence.

10

High prevalence, Low IDU and for sureiillance.

Copyright © 2010, World Health Organization.

All rights reserved. Publications of the World Health Organization can be obtained from WHO Press, World Health Organization, 20 Avenue Appia, 1211 Geneva 27, Switzerland (tel.: +41 22 791 3264; fax: +41 22 791 4857; e-mail: tni.ohw@sredrokoob). Requests for permission to reproduce or translate WHO publications – whether for sale or for noncommercial distribution – should be addressed to WHO Press, at the above address (fax: +41 22 791 4806; e-mail: tni.ohw@snoissimrep).

Bookshelf ID: NBK138578

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