2CKP,1NW1,2CKQ,3ZM9,3LQ3


Conserved Protein Domain Family
ChoK_euk

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cd05156: ChoK_euk 
Click on image for an interactive view with Cn3D
Euykaryotic Choline Kinase
ChoK catalyzes the transfer of the gamma-phosphoryl group from ATP (or CTP) to its substrate, choline, producing phosphorylcholine (PCho), a precursor to the biosynthesis of two major membrane phospholipids, phosphatidylcholine (PC) and sphingomyelin (SM). Although choline is the preferred substrate, ChoK also shows substantial activity towards ethanolamine and its N-methylated derivatives. ChoK plays an important role in cell signaling pathways and the regulation of cell growth. Along with PCho, it is involved in malignant transformation through Ras oncogenes in various human cancers such as breast, lung, colon, prostate, neuroblastoma, and hepatic lymphoma. In mammalian cells, there are three ChoK isoforms (A-1, A-2, and B) which are active in homo- or heterodimeric forms. The ChoK subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).
Statistics
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PSSM-Id: 270705
Aligned: 26 rows
Threshold Bit Score: 412.02
Created: 2-Aug-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:2CKP; Human ChoK A-2 binds ADP; defined at 4A contacts.
  • Structure:3LQ3: Human ChoK Beta binds ADP; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1           #    # #                      # #                              #          
2CKP_A     62 FHISVIRGGLSNMLFQCSLpdttat---lgdepRKVLLRLYGailq-vgaEAMVLESVMFAILAERsLGPKLYGIFpQGR 137 human
1NW1_A     76 LRISRIKGGMSNMLFLCRLsevypp---irnepNKVLLRVYFnpe---teSHLVAESVIFTLLSERhLGPKLYGIFsGGR 149 Caenorhabditis ele...
NP_501732 112 FRIRAITGGMSNLLFLVELpahltp---iqmepEKALLRVHCqs----diDQLLSESVVFTLLSERnLGPKMLGVFpGGR 184 nematode
NP_503573  43 VTVTQITGGQSNILYLATSastkl----spdtpSCFLIRIHRqa-----pSQVFTETVVFSVLSERgLGPKLYGFFpGGR 113 nematode
NP_497879  42 VKVTTILGGQSNHMFHVTSst----------saTPYLLRIHRqg-----pSHVFMDTVNFAIFSERgLGPKLYGFFdGGR 106 nematode
EFA05797   36 IGFKHISGGLSNLLYHISLpeslveeskdlgepQEVLIRVYGqthgehalEALITESVVFTLLSERgLGPKLHGIFpGGR 115 red flour beetle
EFN66565   40 IILKRISGGLSNWLYNVELpegtvp---vrgepRQVLLRLYGqvhgeralEGLITESVIFTLLSERrLGPKLHGVFpGGR 116 Camponotus floridanus
EFX89692   35 MVLKQVSGGMSNFLYYCALppmakp---kqgepSRVLVRFYGqihgegalEALLTESVIFTLLSERkLGPKLHGVFpGGR 111 common water flea
EEB19556   41 ITIKKINGGLSNWLYKITLspfqdp--shdskqNTILLRINGpnygkfakNQKITDSLVFLLMSERgLGPKLLGVFpNGR 118 human body louse
EGT33491   43 VNVVQMTGGQSNLLYLVTAspgkvs---sestpSCFLIRLHCqq-----eSQVFTDTVVFSIMSERgLGPKLYGFFpGGR 114 Caenorhabditis bre...
Feature 1      #### ###                                                                       
2CKP_A    138 LEQFIPSRRLDteeLSLPDISAEIAEKMATFHGMKMPFnKEPKWLFGTMEKYLKEVLRikfteesri-----------kk 206 human
1NW1_A    150 LEEYIPSRPLScheISLAHMSTKIAKRVAKVHQLEVPIwKEPDYLCEALQRWLKQLTGtvdaehrfdlp-------eecg 222 Caenorhabditis ele...
NP_501732 185 FEQFIPSRALQcleISKPGLSKLIAPIVARVHTLDAPI-PKEPQTLQTARQWLERFKKtpagerpiemyltqakvpksdy 263 nematode
NP_503573 114 LEEFLPSRTLDvdsIKLPEIARQVGSLYPKYHDIEVPI-SKSAGALKFIKENLEGYKAlggkvlkmcphsv---kydsmp 189 nematode
NP_497879 107 MEEFLPSRTLDsdcILDPEISRRVGAVYPKYHAIDVPV-SKKRRCFQVMRESLKEYQDlgggdyeikpt-------tvty 178 nematode
EFA05797  116 IEQYINARPLRtgeLADEKLSVKIAQKMAAIHTMEVPLhKEPGWLWDTIDRWLRTCESklksddvpe-----------ff 184 red flour beetle
EFN66565  117 IEEYIPARPLLtkeLADSTLSLLIAEKMGQIHTMQIPIsKEPKWIWETMNNWVNTATDileniedtdiq-------qlen 189 Camponotus floridanus
EFX89692  112 LEEFIPARPLKtkeLVDPEISSIIAEKMAQIHSLDVPIsKEPTWLWDTMNRWMSNMKVtlaaqpthqivdn--dfiekdv 189 common water flea
EEB19556  119 IEEFVDARPLQtseLSKPNISSAIARKMAHIHSLNVPIsKNPDFVYDLMFHWLQELLLhedtykpknl---------elf 189 human body louse
EGT33491  115 LEQFLPSETLDndtVSLPEVARKIGANLPKLHAIEVPI-PKKPRAILMIREFLEECRStgnnvfklvpgsv--kfndsri 191 Caenorhabditis bre...
Feature 1                                             #  #                                    
2CKP_A    207 lhkllsYNLPLELENLRSLLEStp----sPVVFCHNDCQEGNILLLegre----------------------nsekQKLM 260 human
1NW1_A    223 vssvncLDLARELEFLRAHISLsk----sPVTFCHNDLQEGNILLPkassgnirmpslsdetqalgnslsafnpadPRLV 298 Caenorhabditis ele...
NP_501732 264 pstitvAQLERELNFVEFFLQHsh----sPVVFSHNDLQEGNFLLIdgykladdgtv---------ltpdgkptndDPLS 330 nematode
NP_503573 190 seltveQIEQEIATFERWCKVFd-----eTIVFSHNDLAPLNVLELnd---------------------------tKEIV 237 nematode
NP_497879 179 sehpkkISMDDLYKEIDFMEKWtnelfedTVVFCHNDLASSNILELns---------------------------tKELV 231 nematode
EFA05797  185 rnsvdcDFLSEVDWLKKRLEMEn-----cPVVFCHNDMQEGNILIRqdgpen-------------------nnnedPQIV 240 red flour beetle
EFN66565  190 inaikyINLEHEINWLKALVMQqk----yPVTFCHNDMQEGNLLLRqnt-------------------------rkPELV 240 Camponotus floridanus
EFX89692  190 ssamrsILSWKLDSEMDWMKTYltq-lrsPVVFCHNVCINISFLKGftkvndlqegn--------iliresavtreEKLV 260 common water flea
EEB19556  190 kefknyDLLSEVAWLKQYLRKYs-----sVIVFSHNDLQEGNILITndsl----------------------hsnnPSLV 242 human body louse
EGT33491  192 peevtiDELEKEVTLFEKMCSIfd----dTVVFSHNDLWSANILQLne---------------------------tKEIV 240 Caenorhabditis bre...
Feature 1      ##                                                                             
2CKP_A    261 LIDFEYSSYnYRGFDIGNHFCEWMYDYSYekyPFFRANIRKYPTKKQQLHFISSYLPAfqndfen---------lsteek 331 human
1NW1_A    299 LIDFEYASYnYRAFDFANHFIEWTIDYDIdeaPFYKIQTENFPENDQMLEFFLNYLREqgntr-------------enel 365 Caenorhabditis ele...
NP_501732 331 LIDFEYCSYnYRGFDLGNHFCEYGYDYNEaeaPYYKIHQHYFEVEKERKVFCEAYLDEvykmracgdnphfpsdlvtgdr 410 nematode
NP_503573 238 FIDFEYSSYnWRGFDLCMFLCENAFDYRDptpPGLVIDQDYMENHPNLQIFCEAYIDTqykmktanpk---qkfpltegr 314 nematode
NP_497879 232 LIDWEFGSYnCRGFDLAMHLAETAADFRDstpPGIRISEELTDNPPNLQGFCEAYVDAdnklknr----------vpsnr 301 nematode
EFA05797  241 VIDFEYCSYnYRSFDIANHFVEWVYDYTEaeyPFYKEQLENYPSKKQRLLFIKAYLEArg-------------------l 301 red flour beetle
EFN66565  241 LIDFEYCSYnYRGFDIANHFAEWQYDYTVpeyPFFHERPSAGPTKEQKLNFIRAYLKTlgk-----------------eg 303 Camponotus floridanus
EFX89692  261 IIDFEYCSYnYRGFDVANHFCEWMYDYTFdkyPKFSYNPAAMPSVEQQLYFIRYYLETvqremgiddshknpimntidag 340 common water flea
EEB19556  243 LIDYEYCSYnYRGFELANHFLEHTMNYNAedyPHFTIDLSAYPTHEQQMGFIHQYLTTyhellpk---------dsitny 313 human body louse
EGT33491  241 FIDFEYSSYnWRSFDLSMHLSECAFDYRVpfpPGVHVNQIFFENHPNIQLFCDAYIETlykmkqensn---qkyplsdnr 317 Caenorhabditis bre...
Feature 1                                                             
2CKP_A    332 sIIKEEMLLEVNRFALASHFLWGLWSIVQAKiss-ieFGYMDYAQARFDAYFHQKR 386 human
1NW1_A    366 yKKSEDLVQETLPFVPVSHFFWGVWGLLQVElsp-vgFGFADYGRDRLSLYFKHKQ 420 Caenorhabditis elegans
NP_501732 411 eKDLNKIIEESILFMPVSNIFWVCWSLINAEess-iaFDYGAYGRDRLALYFHQKK 465 nematode
NP_503573 315 aAEVESLMLECKFFIPLVNMFWAVWSLRQHLakyeigMDLDVIASDRFSMYFHLKE 370 nematode
NP_497879 302 dLEVSNLICECQFFWPITQLFWACFVMKLALlkyncgVDMDVQAQDRFAVYWHLKE 357 nematode
EFA05797  302 kENPKKILREVEVFTLASHFFWGIWGIINAGtsq-ipFGYWEYAAARLRNYFQQKK 356 red flour beetle
EFN66565  304 sLEEQRVMMEVRIFSLASHLFWSLWSIVNAKisq-ipFGYWDYAACRLKNYMYLKE 358 Camponotus floridanus
EFX89692  341 pQNELRLLREAECYMLASHFFWGLWAVVNAPvss-ipFGYWEYAEARFAAYFEHKQ 395 common water flea
EEB19556  314 aEEEKKLLNEVHQYRLLSHLMWGIWAAAHSQcg--ikFGYWDYSMARLNAYFKTKE 367 human body louse
EGT33491  318 eKEVDRLIQECKFFLPLVNMLWATWSIKNLWtgkqddVDLSVAASNRLSVFFHFKS 373 Caenorhabditis brenneri

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