1FLJ,1CNX,1TB0


Conserved Protein Domain Family
alpha_CA_I_II_III_XIII

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cd03119: alpha_CA_I_II_III_XIII 
Click on image for an interactive view with Cn3D
Carbonic anhydrase alpha, isozymes I, II, and III and XIII. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidines. This vertebrate subgroup comprises isozymes I, II, and III, which are cytoplasmic enzymes. CA I, for example, is expressed in erythrocyes of many vertebrates; CA II is the most active cytosolic isozyme; while it is being expressed nearly ubiquitously, it comprises 95% of the renal carbonic anhydrase and is required for renal acidification; CA III has been implicated in protection from the damaging effect of oxidizing agents in hepatocytes. CAXIII may play important physiological roles in several organs.
Statistics
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PSSM-Id: 239393
Aligned: 10 rows
Threshold Bit Score: 508.903
Created: 1-Nov-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitezinc binding
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1CNX: human carbonic anhydrase II binds zinc and benzenesulfonamide, contacts at 4.5 Angstrom
  • Comment:zinc binding histidines and other residues found to be important for enzymatic activity

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                    #                
1FLJ_A      1 XAKEWGYASHNGPEHWHELYPIAKGDNQSPIELHTkDIRHDpSLQPWSVSYDpgsAKTILNNGKTCRVVFDDtfdrSMLR 80  Norway rat
1CNX        1 MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDThTAKYDpSLKPLSVSYDqatSLRILNNGHAFNVEFDDsqdkAVLK 80  human
NP_571185   1 MAHAWGYGPADGPESWAESFPIANGPRQSPIDIVPtQAQHDpSLKHLKLKYDpatTKSILNNGHSFQVDFVDddnsSTLA 80  zebrafish
AAH04897    1 MAKEWGYASHNGPDHWHELFPNAKGENQSPIELHTkDIRHDpSLQPWSVSYDggsAKTILNNGKTCRVVFDDtydrSMLR 80  human
115454      1 MSHHWGYDSHNGPAHWHEHFPIANGERQSPIAISTkAARYDpALKPLSFSYDagtAKAIVNNGHSFNVEFDDssdkSVLQ 80  chicken
XP_418319   2 AQSVWGYDKENGPEHWHQNYPMAKGDKQSPIEINSkDVRHDtSLSPWHASYDpgaAKTILNNGRTCRVVFDDtfdrSVLR 81  chicken
AAH77855    1 MMHQWGYEDHNGPDVWQDLFPLANGDRQSPINIITkDALYDpSLQPLLVDYDphsAKVISNSGHTVAVEFDDgddsSVVR 80  African clawed frog
NP_001729   2 ASPDWGYDDKNGPEQWSKLYPIANGNNQSPVDIKTsETKHDtSLKPISVSYNpatAKEIINVGHSFHVNFEDndnrSVLK 81  human
1TB0_X      1 MSHHWGYGKHNGPEHWHKDFPIAKGERQSPVDIDThTAKYDpSLKPLSVSYDqatSLRILNNGHAFNVEFDDsqdkAVLK 80  human
Q8N1Q1      2 SRLSWGYREHNGPIHWKEFFPIADGDQQSPIEIKTkEVKYDsSLRPLSIKYDpssAKIISNSGHSFNVDFDDtenkSVLR 81  human
Feature 1                # # #         #            #                                         
1FLJ_A     81 GGPLSGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNp-KYNTFGEAlkqPDGIAVVGIFLKIGr-EKGEFQI 158 Norway rat
1CNX       81 GGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNt-KYGDFGKAvqqPDGLAVLGIFLKVGs-AKPGLQK 158 human
NP_571185  81 GGPITGIYRLRQFHFHWGSSDDKGSEHTIAGTKFPCELHLVHWNt-KYPNFGEAaskPDGLAVVGVFLKIGa-ANPRLQK 158 zebrafish
AAH04897   81 GGPLPGPYRLRQFHLHWGSSDDHGSEHTVDGVKYAAELHLVHWNp-KYNTFKEAlkqRDGIAVIGIFLKIGh-ENGEFQI 158 human
115454     81 GGALDGVYRLVQFHIHWGSCEGQGSEHTVDGVKYDAELHIVHWNv-KYGKFAEAlkhPDGLAVVGIFMKVGn-AKPEIQK 158 chicken
XP_418319  82 GGPLTGAYRLRQLHLHWGSADDHGSEHVIDGVKYAAELHLVHWNp-KHGNFAGAlkqPDGVAVVGVFLKVGktPKPEMKR 160 chicken
AAH77855   81 GGPLMGNYRLRQLHFHWGPSDGHGSEHKVDGVDYAAELHIVHWNseKFSSFVKAacaPDGLAVLGVFLKVGe-PNRYIEK 159 African clawed frog
NP_001729  82 GGPFSDSYRLFQFHFHWGSTNEHGSEHTVDGVKYSAELHVAHWNsaKYSSLAEAaskADGLAVIGVLMKVGe-ANPKLQK 160 human
1TB0_X     81 GGPLDGTYRLIQFHFHWGSLDGQGSEHTVDKKKYAAELHLVHWNt-KYGDFGKAvqqPDGLAVLGIFLKVGs-AKPGLQK 158 human
Q8N1Q1     82 GGPLTGSYRLRQVHLHWGSADDHGSEHIVDGVSYAAELHVVHWNsdKYPSFVEAahePDGLAVLGVFLQIGe-PNSQLQK 160 human
Feature 1                                            #                                        
1FLJ_A    159 LLDALDKIKTKGKEAPFNHFDPSCLFPAcrDYWTYHGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLFASAE--NEP 236 Norway rat
1CNX      159 VVDVLDSIKTKGKSADFTNFDPRGLLPEslDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGE--GEP 236 human
NP_571185 159 VLDALDDIKSKGRQTTFANFDPKTLLPAslDYWTYEGSLTTPPLLESVTWIVLKEPISVSPAQMAKFRSLLFSSE--GET 236 zebrafish
AAH04897  159 FLDALDKIKTKGKEAPFTKFDPSCLFPAcrDYWTYQGSFTTPPCEECIVWLLLKEPMTVSSDQMAKLRSLLSSAE--NEP 236 human
115454    159 VVDALNSIQTKGKQASFTNFDPTGLLPPcrDYWTYPGSLTTPPLHECVIWHVLKEPITVSSEQMCKLRGLCFSAE--NEP 236 chicken
XP_418319 161 ILEEIDNIKTKGKEAPFQHFDPSILFPKsrDYWTYHGSFTTPPCEECITWILLREPIEVSPDQMARLRSLSKNGE--NEP 238 chicken
AAH77855  160 ITDTFGAIRSKGKQSPFTNFDPSCLLPAsmDFWTYPGSLTVPPLLESVVWTVLKDPISISCEQLAMFRSLLSTKEtaGIE 239 African clawed frog
NP_001729 161 VLDALQAIKTKGKRAPFTNFDPSTLLPSslDFWTYPGSLTHPPLYESVTWIICKESISVSSEQLAQFRSLLSNVE--GDN 238 human
1TB0_X    159 VVDVLDSIKTKGKSADFTNFDPRGLLPEslDYWTYPGSLTTPPLLECVTWIVLKEPISVSSEQVLKFRKLNFNGE--GEP 236 human
Q8N1Q1    161 ITDTLDSIKEKGKQTRFTNFDLLSLLPPswDYWTYPGSLTVPPLLESVTWIVLKQPINISSQQLAKFRSLLCTAE--GEA 238 human
Feature 1                            
1FLJ_A    237 PVPLVGNWRPPQPIKGRVVRASF 259 Norway rat
1CNX      237 EELMVDNWRPAQPLKNRQIKASF 259 human
NP_571185 237 PCCMVDNYRPPQPLKGRKVRASF 259 zebrafish
AAH04897  237 PVPLVSNWRPPQPINNRVVRASF 259 human
115454    237 VCRMVDNWRPCQPLKSREVRASF 259 chicken
XP_418319 239 MCPLVDNWRPLQPVKGRIVRASF 261 chicken
AAH77855  240 SCCMTTNHRPVQPLKNRKVRASF 262 African clawed frog
NP_001729 239 AVPMQHNNRPTQPLKGRTVRASF 261 human
1TB0_X    237 EELMVDNWRPAQPLKNRQIKASF 259 human
Q8N1Q1    239 AAFLVSNHRPPQPLKGRKVRASF 261 human

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