Conserved Protein Domain Family
Exo5

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pfam09810: Exo5 
Exonuclease V - a 5' deoxyribonuclease
Exonuclease V is a monomeric 5' deoxyribonuclease that is localized in the nucleus. It degrades single-stranded, but not double-stranded, DNA from the 5'-end, and the products are dinucleotides, except the 3'-terminal tri- and tetranucleotides, which are not degraded. The initial hydrolytic cut of exonuclease V on the dephosphorylated substrate produces a mixture of dinucleoside monophosphates and trinucleoside diphosphates. The enzyme is processive in action. Exo5 is specific for single-stranded DNA and does not hydrolyze RNA. However, Exo5 has the capacity to slide across 5' double-stranded DNA or 5' RNA sequences and resume cutting two nucleotides downstream of the double-stranded-to-single-stranded junction or RNA-to-DNA junction, respectively.
Statistics
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PSSM-Id: 430843
Aligned: 64 rows
Threshold Bit Score: 237.553
Created: 23-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
GAA87462             175 SPVERF-RRPPMKAFSVTDLISPAWCELQYWYTLTKF----GRKPRTAAMKLGSKIHKTLEDEI---Y----------TT 236 
XP_011127614         281 SPLDRWrKRGSNTKLSVSDLLCNMWCEQQYHYTLLR-----GFKRRTAEMQAGTKIHREMEEEV---H----------TI 342 
XP_011109951         263 SPLDRWrSKAGLSKLSVSDLLSNMWCEQQHHYTLLR-----GFKRRTAEMQAGTKIHREIEEEI---H----------TV 324 
WGS:AATM:SJAG_05280   79 nlyeMF-RSD-RGYLKVTDMVAPLWCGVQHSYALLD-----RVERKTPAMEKGTIIHLKLELETtppK----------ER 141 
EPY53777              63 tlydlF-RKR-RGYLNVTDLVSPLWCEVQQDYYLTK-----KIKKTSPQMIKGSSVHQHLEYETtppT----------DR 125 
Q9Y7L4                63 SLFQLF-RKH-KGYLNVTDLVLPLWCEVQHEYYLLR-----RIKKKTPKMERGIKLHQILEYETsppS----------ER 125  Schizo...
Q6CHE7                71 lQLKAL-KNPGSNRVSVTAASAP-FCEVRTLYELAEVa--vEEEKPTNAMERGAQVHNMAEYSL---Y----------EA 133  Yarrow...
XP_002141959          49 vPIQKY-RD--KSQIGVTTLSRQIWCEKSLELSLEY-----DVIASSKAIDEGVRHHEILELQD---H----------DI 107 
CCC68314             106 spfidthKSQGINRLSVTKLLTKSWCELRFTYDMYAQaglqIQPPPDKFLTSGTSIHGSLETAL---Hpgl--------- 173 
A7TMJ6               113 nsdgngeneKVRNRLSVTKLLTKRWCELREAYDIYSE----TPLFEHKQIIEGKLVHQKLEEDI---HpvtedlesfvED 185  Vander...
GAA87462             237 VPVDI-----TTKEDALALRIWNIIQGLRMLRE-------------FGITRELEVWGL-------V-DG-----E----L 281 
XP_011127614         343 VPVTV-----KTSHDKWGLRIWNMIQGLESLRA-------------TGLTRELEVWGW-------I-DG-----V----F 387 
XP_011109951         325 VPVTV-----KSRHDKWGLRIWNMIQGMESLRT-------------VGLTRELEVWGW-------I-DG-----V----F 369 
WGS:AATM:SJAG_05280  142 VRTDE-----TLAEEPWALHILLQLDGLLLLTK-------------NGITREFPIWGF-------YkDS----------L 186 
EPY53777             126 ILLNK-----TSKEEPWALKILKQIEGLQQLYL-------------NGITREFPIWGL-------YkDS----------F 170 
Q9Y7L4               126 RVLDR-----TSKEEPWALRLLRQLEGIMLLQK-------------NGITREFPIWGY-------YkES----------S 170  Schizo...
Q6CHE7               134 TEVSLpvppaTTPTEEWYQKLMNMYLKLSMLRTdsapavnelemspTFMAREIPVFGN-------Y-KN-----T----R 196  Yarrow...
XP_002141959         108 IDVIV-----ETSIEKLGLELLSIANLLEALYE-------------RGYVRELPIIGF-------H-KD-----I----M 152 
CCC68314             174 --------ldWNLEHDLFDVWFDSLLKMANLFQ-------------GGQCREIIAHGYvnsndltLlDGdmdvtDeddvL 232 
A7TMJ6               186 FEVPIp----TDNFHNHVDDLFSCSMRLLSLFR-------------CGEAREVRCHAFldsrtgtFiDGlp--kDgkdvL 246  Vander...
GAA87462             282 VTGVIDQL---SHDCPDP----ELEATAAT-----------------------------------------------YYA 307 
XP_011127614         388 INGVIDEV---NFKKFERdr-dDKENGAVGrvgdrgeakevslpenvteamitgaapmeekagdiletpkkkrgrprKTP 463 
XP_011109951         370 INGVIDEV---NFTKFRNeardEKENQKVEdiddkaeelevalakndpdtii---tvvgesskeipetpkkkrgrpkKNP 443 
WGS:AATM:SJAG_05280  187 LFGIIDELtvlKTEKDDN-------------------------------------------------------------- 204 
EPY53777             171 VVGIIDEI---SLAQQGT-------------------------------------------------------------- 185 
Q9Y7L4               171 IFGIIDEI---SLNNPSK-------------------------------------------------------------- 185  Schizo...
Q6CHE7               197 LFGIMDLI---YWRNK---------------------------------------------------------------- 209  Yarrow...
XP_002141959         153 LRGIIDSL---KLIHNNNl--eMLDSLNFNnynsk--------------------------------------------- 182 
CCC68314             233 ISGVIDHL---TLRGKDVrl-------------------------------------------------------fpDVN 254 
A7TMJ6               247 VSGIIDHL---SLRRKIRvftssgftef----------------------------------------nglndevfeNGN 283  Vander...
GAA87462             308 DVEASRAILPEYQMSLSDYLLSPTgggrllsevygqddtpakadgfdmdtdmnlasssqetdnFPHQPRIYLTDVKTRGs 387 
XP_011127614         464 EPTSPAPKTPKQQKTILDFITPSPkk------------------------------------tRSKGQPAYVLDLKTRA- 506 
XP_011109951         444 DPVSPEPATPKQQSSILDFITPSPkk------------------------------------sRSKGKPAYVLDLKTRF- 486 
WGS:AATM:SJAG_05280  205 --------------------------------------------------------------------LLSMKDNKTRG- 215 
EPY53777             186 --------STKEHSDIRDFWLP-------------------------------------------GTRNIYFTDNKTRF- 213 
Q9Y7L4               186 ---------SNFNSDIRNYFNF-------------------------------------------KMYDLSFVDNKTRF- 212  Schizo...
Q6CHE7               210 --------------------------------------------------------------------KLNICDMKTTY- 220  Yarrow...
XP_002141959         183 ---------------------------------------------------------------miqkyEVLITDTKTRR- 198 
CCC68314             255 DIGALLMELDKHRELIKGDT------------------------------------------------NILVSDVKTRS- 285 
A7TMJ6               284 NFQSIIEWLNANIDFLKSEY------------------------------------------------QINVSDVKTRM- 314  Vander...
GAA87462             388 gS--VPTvkSSSFRPTLLQLQLYYHMLNRLVLsDD---------VT-----------------------IDLLASRY--- 430 
XP_011127614         507 -SarIPEpgSSQSLSVHIQLMLYRQLLNDMIR------------AT-----------------------PKSMVKKLck- 549 
XP_011109951         487 -SsrIPEsgSSQSKSVHIQLMLYRQLLNYMIK------------AT-----------------------PKSMVRRLcl- 529 
WGS:AATM:SJAG_05280  216 -TitIPS--QSQILGSELQLMYYYHLFQEYFQnADs--------------------------------wWDSFLKQQ--- 257 
EPY53777             214 -SnaKPS--ISQVFQSKIQVMYYAHLFLHYFP--S---------LNikstqplyatqkddnqmklekpwMDSFLNSL--- 276 
Q9Y7L4               213 -SsrKPG--ASQILSSKVQLMYYVHLFLNYFPsLGekqqsifrdISptysnrl----------hsqshwWNMFLSQL--- 276  Schizo...
Q6CHE7               221 -RrsVPE--EPQMTSHIHQTMLYHYLFSDMCR-------------D-----------------------IDATMGEYlrd 261  Yarrow...
XP_002141959         199 -KpsTPS--IPQQKTTVFQLGLYKLLLNRMRLaGLewknkhscnINfsesydln-------eccegckfLYKLFEFN--- 265 
CCC68314             286 -InrLPTq-GTVLKATKLQLMYYKFFLKSFG-------------LN-----------------------QESTYKKLlvn 327 
A7TMJ6               315 -FrsVVSq-KSVLKYSKYQVMYYRYFLELLG-------------LH-----------------------PDVTYGQLlns 356  Vander...
GAA87462             431 ----NLDPNRPF-----------TDAFINE-VGGLN---DQFFDAMSS--QEfdpdyiptpqdaiehpq----------- 478 
XP_011127614         550 --hhDLPADEPF-----------SDDLIAG-LAGNGnetQPGSDEL---------------------------------- 581 
XP_011109951         530 --qqDIPADEPF-----------SDDMIAE-LAGLDngtEPGSDDL---------------------------------- 561 
WGS:AATM:SJAG_05280  258 ----DLDGKKNL-----------GQNFLKQaIETLPsidP---------------------------------------- 282 
EPY53777             277 ----NLDGSKDL-----------GTEFLEQsIRTIPtipE---------------------------------------- 301 
Q9Y7L4               277 ----SLDGTKDL-----------GPKFLEQsILSIPdipE---------------------------------------- 301  Schizo...
Q6CHE7               262 diciDTDVRSEF-----------SAAYEGQ-------------------------------------------------- 280  Yarrow...
XP_002141959         266 ----KVDYMIQF-----------NE------INKLEisrNKENKYEDSqnKNiigdyqdkniigdyqdkniiednqdkni 324 
CCC68314             328 aqrrGIDVDAPIdpmkllnfmisSNGFLNDdMRKMRdgfDIGFPLFDNenSSnnnndaefnmsn---------------- 391 
A7TMJ6               357 slsrGFNIDQRIdpakviyfmasDEVIVDD-MRKLRdgdDIGFPPFDSdfTGsspteeeydm------------------ 417  Vander...
GAA87462             479 --------rhlpssqsqpnshsssQSSSEDST-------TILSTHNTLTTLWTL---MK---SHLRLTFLpspptaspsi 537 
XP_011127614         582 ---------------------------------------SLLLENNSIEKLYTL---FQ---KKLKQTI----------- 605 
XP_011109951         562 ---------------------------------------TVLLENNSIEKLYTL---YH---RRLKQTI----------- 585 
WGS:AATM:SJAG_05280  283 ---------------------------------------KELEAHNTLSGLHALatrLT---KDFQLRS----------- 309 
EPY53777             302 ---------------------------------------DDFKENNSLNGLYKIsskLA---RRLRLNI----------- 328 
Q9Y7L4               302 ---------------------------------------DVFAGHNSLNGLYALvfaSA---KKLHLRL----------- 328  Schizo...
Q6CHE7               281 -------------------------SGPADTI------------PTSISGLIKL---IG---DELPKPR----------- 306  Yarrow...
XP_002141959         325 iednqdkniiednqdkniiednqdKNIIEDNQdkstiliSEILS--NFQNLLHIgfkLM---NLLAIMP----------- 388 
CCC68314             392 ---------------------------ipsidsevgvnnNILEKYEEFMTSWTRpvnLKyfaARMSQFYYn--------- 435 
A7TMJ6               418 -----------------------------silsdqitdpNVLERYGEFLVPWKRpvtLKyfaARLAQMYNc--------- 459  Vander...
GAA87462             538 psefqprsldpyPTILSPLLTARYISSKpsa------------dlqPPRVLGSRSFLFDPTSMSSYVADQMHWWRGERDP 605 
XP_011127614         606 -------------STVSPDLTVVYRWQQ------------------NGQFMDSADYMADDDMLKQHVDNIISWWKGERET 654 
XP_011109951         586 -------------STIAPELTVVYRWQR------------------NGEFLDSADYMADDTYLKEHVDNVIAWWKGERAT 634 
WGS:AATM:SJAG_05280  310 ---------------LSPKLTVTYRHNI------------------SGDVIRNHIFDMNSTILNHYFHEMFAYWHNEAEP 356 
EPY53777             329 ---------------SDKNLGITYWNPQ------------------TQQPLHVEKFNYEPTTLDDCYNRVNSLWRTMREP 375 
Q9Y7L4               329 ---------------TDDNLTIAYRNDK------------------TGEVVYKDKFSFSNKLLEASYTKAYQFWHNLREP 375  Schizo...
Q6CHE7               307 --------------SLAKTVSVEYVKRSedrvpeeyqdlyplsdytQRQSFAVVQAPVDYKELKRVMSETLPFWHGQRAA 372  Yarrow...
XP_002141959         389 --------------PIHNNMKVEYDCE--------------------GCTFATKWHTCEELTIMTEVDYLLGWWLGLRDS 434 
CCC68314             436 -----------iGQLISDELMVEYYHK--------------------NENFHNILFKYDNKMLKEHVTDSGKYWFGKRDI 484 
A7TMJ6               460 -----------iSPLLSKHLTLEYYYK--------------------GDNFKNINFDFNEDEIRNGAFDSSMFWFGKRDI 508  Vander...
GAA87462             606 RGVEVMEA-WK--CRICDFRDDCEWR 628 
XP_011127614         655 VGVPIEEA-WK--CKRCEFSDNCEWi 677 
XP_011109951         635 VGVEINET-WK--CNKCEFMEDCIWR 657 
WGS:AATM:SJAG_05280  357 KGVPENET-FK--CKYCEFSERCWWl 379 
EPY53777             376 VGVSPNEV-FK--CRSCEFREECWWl 398 
Q9Y7L4               376 EGVPAEEV-YK--CRSCEFQKECWWl 398  Schizosaccharomyces pombe 972h-
Q6CHE7               373 IGVDADSM-NK--CRYCQFRPVCGWY 395  Yarrowia lipolytica
XP_002141959         435 EPVLNSEM-WK--CKYCNVLEYCNfs 457 
CCC68314             485 EPIKPNLKnFKifCKNCDYSHVCSWR 510 
A7TMJ6               509 EPIEPTSEnLItyCKYCDYVNVCSWR 534  Vanderwaltozyma polyspora DSM 70294
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