3GGZ


Conserved Protein Domain Family
Ist1

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pfam03398: Ist1 
Regulator of Vps4 activity in the MVB pathway
ESCRT-I, -II, and -III are endosomal sorting complexes required for transporting proteins and carry out cargo sorting and vesicle formation in the multivesicular bodies, MVBs, pathway. These complexes are transiently recruited from the cytoplasm to the endosomal membrane where they bind transmembrane proteins previously marked for degradation by mono-ubiquitination. Assembly of ESCRT-III, a complex composed of at least four subunits (Vps2, Vps24, Vps20, Snf7), is intimately linked with MVB vesicle formation, its disassembly being an essential step in the MVB vesicle formation, a reaction that is carried out by Vps4, an AAA-type ATPase. The family Ist1 is a regulator of Vps4 activity; by interacting with Did2 and Vps4, Ist1 appears to regulate the recruitment and oligomerization of Vps4. Together Ist1, Did2, and Vta1 form a network of interconnected regulatory proteins that modulate Vps4 activity, thereby regulating the flow of cargo through the MVB pathway.
Statistics
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PSSM-Id: 460910
Aligned: 113 rows
Threshold Bit Score: 147.221
Created: 21-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
3GGZ_D         13 KTCLKMCIQRLRYAQEKQQAIAKQSRRQVAQLLLTNKEQKAHYRVETLIHDDIHIELLEILELYCELLLARVQVINDIST 92   baker's yeast
ACN34775       22 KTLLKLSIPRIKLLRNRRELQLRQMRRDIAKLLEAGQEATARIRVEHIIREENMMAAQEILELFCELIAVRLPIIEAQKE 101 
XP_009394434   18 KTCLNLAISRIKLLRNKRELQLKQMRKEISQYIQTGQEAIARIRVEYIIREQNILAAYEIIELFCEFVLARVPVLESQRD 97  
XP_004236982   18 KTCLTLAISRIKLLQNKRDAQLRLMRKEIAQFLQTGQEAIARIRVEHIIREQNVWAAYEILELFCEFVFARVPILESQKE 97  
XP_009103065   18 KTSLNFAIARMKLLQNKRDMHLKHMKKEIAQFLQAGQEPIARIRVEHVIREMNLWAAYEILELFCEFVLARVPILESQKE 97  
XP_009401974   21 KTSLKLAVSRMKLLKNKREASVRQMRRDLAQLLESGQEQTARIRVEHVIREEKTMAAYDLIQIYCELIESRLPIIESQKN 100 
XP_009102144   15 KTALQMANSRLKILKNKKEIQIKQLRRELAQLLEAGNTPTARIRVEHVVREEKTVAAYELIGIYCELIVVRLGVIESQKN 94  
XP_020147550   22 KIALKMAMARIKLLRNKKEVQVRQMRREVAQLLDGNQDQTARIRVEHVIREEKFMQAYDLIEVYCELIVARMSIIDSQKT 101 
EEF48119       12 KTALKLAVSRIKLLKNKRDAQVKQLKRELAQLLESGQDRTARIRVEHVVREEKTLAAYDLIEIYCELIVARLQIIESQKN 91  
KRH60150       13 KTALTLAVPRIKLLKNKREANVKQLRRELAQLLHSGHNHAARVRVEHVVKEEKTMAAYDLIKIYCDLIAARMPMIESQRN 92  
3GGZ_D         93 eeqlvkehMDDGINEAIRSLIYAILFVdEVKELSQLKDLMAWKINVEFVNGVIAD-HI-DVPEKIIKKCSPSVPKEELVD 170  baker's yeast
ACN34775      102 --------CPIDLKEAISSVCFAAPRCaDLPELMQVQMMFATKYGKEFVAAASELmPDcGVNRQIIELLSIRPPPVDAKL 173 
XP_009394434   98 --------CPLELQEAISSIIFASPRCsDLPELLHIRNLFSTKYGKEFVAAASELrPEsNVNRMIIEKLTARAPPAELKI 169 
XP_004236982   98 --------CPSELREAVASIIFAAPRCsDLPDLLHVRNLFAAKYGKEFIAAASELrPDtSVNRTIVEKLSVGAPSAELKL 169 
XP_009103065   98 --------CPRELREAIASIIFAAPRCsEVPDLLQLKNLFGTKYGKEFIMVSSELrPDsGVNRTIIEKLSPASPSGETRL 169 
XP_009401974  101 --------CPIDLKEAISSVIFASPRCaDIPELMDIRKQFQAKYGKDFVTAAIEVrPEcGVSRMMVEKLSAKAPDVQIKI 172 
XP_009102144   95 --------CPIDLKEAVASVLFASQRLsDVPELSEIVKQFTTKYGKDFATSAIELrPDsGVSRLLVDKLSVKAPDGPTKV 166 
XP_020147550  102 --------CPIDLKEAIASVIFASMRCsDVTELADVRKNFTSKYGKEFATSALEVrPDsGVNRLVIEKLSAGAPDVQTKT 173 
EEF48119       92 --------CPIDLKEAISSVLFASPRCaDVPELMDVRKHFTAKYGKEFVSAAVELrPDcGVSRLLVEKLSAKAPDGPMKM 163 
KRH60150       93 --------CPIDLKEAISSVIFASPRCsDIPELVVVKKHIMAKYGREFVSAAVELrPDcGVNRLLVEKLSTSAPDGPTKI 164 
3GGZ_D        171 LYLKEIAKTYDVP 183  baker's yeast
ACN34775      174 KLLKEIAEEHEVD 186 
XP_009394434  170 KVLKAIAQEYNLV 182 
XP_004236982  170 NVLKEIAKEYNVE 182 
XP_009103065  170 KVLKEIAKEYSLN 182 
XP_009401974  173 KVLNEVAKEHDVK 185 
XP_009102144  167 KILTEIAEEHNVV 179 
XP_020147550  174 KTLSSIAAEHNIK 186 
EEF48119      164 KILSAIAEEHNVK 176 
KRH60150      165 RILTAIAEEHNVQ 177 
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