1U83,1QWG


Conserved Protein Domain Family
ComA

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pfam02679: ComA 
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(2R)-phospho-3-sulfolactate synthase (ComA)
In methanobacteria (2R)-phospho-3-sulfolactate synthase (ComA) catalyzes the first step of the biosynthesis of coenzyme M from phosphoenolpyruvate (P-enolpyruvate). This novel enzyme catalyzes the stereospecific Michael addition of sulfite to P-enolpyruvate, forming L-2-phospho-3-sulfolactate (PSL). It is suggested that the ComA-catalyzed reaction is analogous to those reactions catalyzed by beta-elimination enzymes that proceed through an enolate intermediate.
Statistics
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PSSM-Id: 426922
Aligned: 98 rows
Threshold Bit Score: 215.059
Created: 21-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
1U83_A        31 PVRTNKPRETGQSILidNG-----YPLQFFKDAIAG------ASDYIDFVKFGWGTSLLTKD--LEEKISTLKEHDITFF 97  Bacillus subtilis
EGS20077      55 NTRPPKPRKVGVTEI--RGsyysvMGKRYLKDVLET------MSYNIDGLKFGGGSFSLYPEpqLRELIDLAHEHGVYVS 126
XP_003651023  64 NTRPPKPRKVGVTEI--RGpyysaMGKHYLKDVLETshtpssMGHHIDGLKFAGGSFALFPEdkLRELIELAHEHGVYVS 141
EON62092      24 NPLPPKPRKTGVTEI--RGpyysvMGKRYLSDVLET------MGPHVDGLKFAGGSFSLFQEdrLRELIDLAHEHGVYVS 95 
EKG18638      80 NPRPPKPRTTGVTEI--RGpyysaMGPHYLSDVLHT------MGHQVDGLKFAGGSFALFPEkqLRELIEIAHQHGVYVS 151
ELR07797      56 NPRAPKPRKLGLTEM--RGpyysaYGTRHLEDVFET------MGTHVDGLKFAGGSFAVMPEgrVREMIEVAHRNGVYVS 127
EHY51966      59 NPRTPKPRNRGVTEI--RGpyysaYGKRHLQDVLET------MGYHVDGLKFAGGSFALMPEgaVRELVDLAHQHDVYVS 130
EFW22144      62 NRLSSKPRTRGVTEI--RGpyyavMGRRYLADILET------MGEYVDGLKFSGGSFSLFEEekLREMIDLAHEHNVYVS 133
EAW15135      61 NPRPPKPRSKGVTEI--RGpyytvMGKRYLADVLET------METHVDGLKFAGGSFTLFPEkpLRELIDLAHEHGVYVS 132
EQB56491      66 NPRDPKPRTKSVTEI--RGpyysvMGKRYLEDVLET------MGHYVDGLKFAGGSFSLFPEdkLKELIDLAHQHDVYVS 137
1U83_A        98 FGGtLFEKYVSQK------KVNEFHRYCTYFGCEYIEISNGTLPXTNKEKAAYIADFSDE-FLVLSEVGSKDAe------ 164 Bacillus subtilis
EGS20077     127 TGG-WIEHLLTHPsp--hdIITRYLTKCRSLGFDVIEISSGFLSIPADDWLRLVDLVHSEgLIAKPEIGIQFGaggdtdv 203
XP_003651023 142 TGG-WIEHLLTQSdp--atAIPRYLKKCKALHFDVVEVSTGFLSLPPDDFVRLVERVQAEgLTAKPEIGIQFGaggdtda 218
EON62092      96 TGG-WIEHVLTGAggdvsgTVDRYLQKCKDIGFDVVELSTGFLSLPAPDWLRLVDRVHSFgLKAKPEIGIQFGaggdtaa 174
EKG18638     152 TGG-WIEHVLAGCggdvgsGVDKYLDKVRELGFDVVEVSTGFLSLPEGDWLRLVERVQERgLRAKPELGIQFGaggdtea 230
ELR07797     128 TGG-FIEHILTHAdv--hsVVDRYLEQCKDLGFDVVELSSGFLSFPPDDWLRLVEKVQKHgLKPKPELGIQFGaggdtea 204
EHY51966     131 TGG-WMEHVLTQPda--ntVVEKYLRKCKDIGFDVVELSAGFLSFPPDDWLRLVDKVHSMgLKAKPELGIQFGaggdtaa 207
EFW22144     134 TGG-WAEHLLTHPdn--atVLEKYFAKCKELGFDVIELSAGFLSFPGDDWLRLVDKVHSYgLKAKPELGIQFGaggdtsa 210
EAW15135     133 TGG-WAEHLLTHPdp--ntVFDKYLKKCKDLGFDVVELSSGFLSLPEDDWLRLVDKVHSHkLKAKPELGIQFGaggdtpt 209
EQB56491     138 TGG-WIEHVLTQSap--ltAVDRYLETCKRLGFDVIELSTGFLSLPPSDWLRLVERVQSFnLTPKPEIGIQFGaggdtaa 214
1U83_A       165 --lASR-----------QSSEEWLEYIVEDX-EAGAEKVITEAResGT-GGIcsssGDVRFQIVDDIISSdIDINRLIFE 229 Bacillus subtilis
EGS20077     204 aslESVeg-------gtSGPEKVVALGKKFIaQCGVERIMIESE--GItENV----RKWRTDVVRGILEG-LPKENVMFE 269
XP_003651023 219 aelEALsrsgggggggtSDPSKLVELGRRFVdQLGVERVMIESE--GItENV----RAWRTDVVRQVLRG-LPQEKVMFE 291
EON62092     175 gdlESMg---------tSDPSRVINLAKQFV-DAGVERMMIESE--GItENV----KEWRTDVIQAILKE-LPMDKVMFE 237
EKG18638     231 enlEAIgs--------sSDPSKVVTMAKKFV-AAGVERCMIESE--GItENV----KSWRTDVIQAILRE-VPLENVMFE 294
ELR07797     205 salEKTg---------tSDPAKLVEMGRKFL-AAGVEVMMIESE--GItENV----RTWRTDVIQKILRD-LPMETVMFE 267
EHY51966     208 edlEAAg---------tSDPSKVVNLGKKFI-EAGVERMMIESE--GItENV----KTWRTDVIQAILRD-LPLEKVMFE 270
EFW22144     211 eelEALg---------tSDPGKLINLGRRFL-DAGVERLMIESE--GItENV----KSWRTDVVSTIMKE-LPPERVMFE 273
EAW15135     210 sslEAIg---------tSDPVKLVNMGRKFL-DAGVERLMIESE--GItENV----ESWRTDVVSTIMKE-LPSERVMFE 272
EQB56491     215 adlQSMgs--------qSDPAKVVDLGKRFI-DMGVERIMVESE--GItENV----ASWRTDVIQKILRD-LPMEKVMFE 278
1U83_A       230 APNKTLQQGFIQKIGPNVNLaNIPFHDAIALETLRL 265 Bacillus subtilis
EGS20077     270 AAEPKVFNWYVREFGADVNL-FVDHSQIVQLSALRE 304
XP_003651023 292 AAEPRVFNWYVREFGADVNL-FVDHSQIVQLSCLRE 326
EON62092     238 AAEPAVFNWYIREFGVDVNL-FVDHSQIVQLACLRA 272
EKG18638     295 AADPTVFNWYVREFGVDVNL-FVDHSQIVQLSCLRH 329
ELR07797     268 AAEPKAFNWYVREFGVDVNL-FVDHSQVVQLACLRS 302
EHY51966     271 AADPPVFNWYIREFGIDVNL-FVDHSQIVQLTCLRS 305
EFW22144     274 AADPSVFNWYIREFGVDVNL-FVDNSQIVQLSCLRR 308
EAW15135     273 AADPKVFNWYVREFGIDVNL-FVDHSQIVQLSCLRQ 307
EQB56491     279 AADPAVFNWYVREFGVDVNL-FVDHSQIVQLSGLRA 313
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