Conserved Protein Domain Family
EcoRI_methylase

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pfam13651: EcoRI_methylase 
Adenine-specific methyltransferase EcoRI
This methylase recognizes the double-stranded sequence GAATTC, causes specific methylation on A-3 on both strands, and protects the DNA from cleavage by the EcoRI endonuclease.
Statistics
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PSSM-Id: 404530
Aligned: 16 rows
Threshold Bit Score: 455.675
Created: 25-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
WP_006193129       11 LSKARDAKKDEFYTAYEDIQAEMNHY----EDKFQGKTVLCNCDDPFESNFCKFFLRNFNYLGLKRLICTSYSTSPFIGQ 86  Selenomona...
WP_004219155        5 LNRANQAKEDEFYTQLSDIESELKHY----RKHFAGKTVLCNCDDPFESNFFKYFCLNFNRLKLKKLIATCYSGSPITGN 80  Bifidobact...
ACL33436            7 LHRANREKNDEFYTRLVDIENELRHY----TQHFKDKIIFCNCDDPEESNFFRYFALNFEHLGIKKLIATHFDANEP--- 79 
WP_011967238       10 LQKAKKSKSDEFYTQLCDIERELQYY----QGQFEDKIVYCNCDDPRVSNFFKYFAANFHNLGLKKLIASCYIE------ 79  Parabacter...
tigr:CHAB381_0064   7 LRRANKAKEDEFYTQLSDIERELRHY----KNHFKDKVVFCNCDDPYESNFFKYFANNFNHLGLKRLIATCYGTSSIKFE 82 
jgi:Dehly_1157     37 LSAAKAAKQDEFYTQYVDIQKEIEAYlefdPNTFRGKVVYCNCDDPFESNFFKYFAANFNKLGLKKLITTSYDGSPIAGQ 116
CDD20295            6 LSNAKAAKKDEFYTMFYDIETEMEAYldynPDVFRGKTVLLPCDDPEWSNFTRYFAQNFEELGLEKLISTSYAIESKAYK 85 
CDE57090            6 LRTAQSGKNDEFYTQYHDIEVEMNAYleynPDVFKDKTLLLPCDDPEWSNFTRYFAAKFDSLGLKRLISTSYAPESKKMI 85 
CCY26553            7 LNSAKTARKDEFYTQLTDIEKEMRYY----RKHFKGKTVLCNCDDPFESNFFKYFVLNFNRLGLKKLIATCYSTSPIMGS 82 
WP_013065369        6 LNAAKTAKKDEFYTQMTDIERELQHY----WPHFRDKVVLCNCDDPYESNFFKYFALRFNQLGLKKLICTCYNGSPVQGN 81  Prevotella...
WP_006193129       87 QLtlfdgadepvvrgH---------------GYVMDVKEVPMANGRGVSD-EDIDALlkskkrgvkKLKGD-----GDFR 145 Selenomona...
WP_004219155       81 ELt-----------lFgddte-----eerrtPYKAVVTSVRDANGDGATSmVDVAELfkqgenlceRLEGN-----GDFR 139 Bifidobact...
ACL33436           80 -------------------------------TYKLEIDRELDLNTDGKIDfQDIQRI---------PLQQN-----GDFR 114
WP_011967238       80 ---------------Sdnsifn---eqnfktGFYCEYFG----KSNQ-----NIEII---------NFKGN-----GDFR 118 Parabacter...
tigr:CHAB381_0064  83 QLs-----------vFdinyie---dknessPYKVIIDNVEDVNNDGAIGiEDIRQImqqnkntvtLLEGD-----GDFR 143
jgi:Dehly_1157    117 LN-------------LfpeynegngkrqkpkAVAFIIDHVKDEDGDGAANvTDVSLFlkrnkaariALKGNdqypgGDFR 183
CDD20295           86 TGiql-------sflEtespkf---dksktnTHGKISVLEHDTTGDGRINlDDLEWD---------YLEGD-----GDFR 141
CDE57090           86 LGglf-------seyEqsspqf---dvdkskTHGKIYVLDSDITGDGHIDiDDLQWK---------YLDGD-----GDFR 141
CCY26553           83 HFhyhvdgsgqisfsFedeavi---dndskrPYKATVTQVYDTTGDGGVDmLDVAKLfmtgdnelvELNGD-----GDFR 154
WP_013065369       82 ELm------------Idfgdfs---depkkvAHKVVITEVKDLNGDGAVDlSDVQYLlqndknvisILKT------GDFR 140 Prevotella...
WP_006193129      146 SEECIDYLKQADVVVTNPPFSLFREYVAQIMVYQKDFLIIGNVNAITYKDIFPLIKNNKLWLGAsiHSGDRKFYVPNDYP 225 Selenomona...
WP_004219155      140 SEECLRLLDEADIVVTNPPFSKFREFVSTLVEHDKKFIIIGNQNAITYKEFFPLLRDNKVWLGY--GCGDMAFKVPDDYE 217 Bifidobact...
ACL33436          115 SPECIEILKQSDIVVTNPPFSLFREYVAQLMEYEKKFVIVGNQNAITYKETFKLIKENKIWLGN--KSGDMAFRVPDYYE 192
WP_011967238      119 SAESIELLKQSDIVVTNPPFSLFREFVSQIIKYNKKFLVIGNVNAITYKDIFELIQRNKAWLGVnlGRGISGFIVPDHYE 198 Parabacter...
tigr:CHAB381_0064 144 SEECIELLKQADIVVTNPPFSLFREYVDLLISYKKSFLIIGNQNAITYKEIFSLLKKDKIWLGY--YAGDMEFQVPDYYE 221
jgi:Dehly_1157    184 SPECLAFLKETDIVVTNPPFSLFREYVAQLMEYGKKFVIIGPKSAITYKEIFPLIKESKLWLGSgfANGNAYFSIPTHAC 263
CDD20295          142 SDEVKKLRDEADIIVTNPPFSQFRDFLAWIVEAKKQFIIIGNVNCITYKEVFPLIKENKIWLGVtnFNRGMFFRVPDDFE 221
CDE57090          142 SAEVTALRNQADIIITNPPFSLFREFLAWITEADKQFIIIGNINAITYKYTFPLIITNKMWLGLtiHSGDREFEVPQEYP 221
CCY26553          155 SPECIELLDEADIVVTNPPFSLFREYLGTLVEHQKHFIIIGNVNAVKYQEVFPLFMSNELWLGAsiHSGDRAFFVPDDYP 234
WP_013065369      141 DPECIELLKEADIVVTNPPFSLFREYISQLMKYEKKFIVMGRMSALHYSEIFPLFTQNKLWCGFnfNFS-AVYKAPYENT 219 Prevotella...
WP_006193129      226 LRAAGCGVE-DDG----RKYIRVKGVRWFTNLDYAMRHEELVLYK----------RYygNEEEYPKYDNYDAINVDKTAD 290 Selenomona...
WP_004219155      218 PRKTRYWQD-ESG----QKWRSMGNACWYTNLDIKKRHEDLVLVK----------RY--NRDDYPKYDNYEAINVGKVID 280 Bifidobact...
ACL33436          193 EKATRYWQD-ETG----QKWRSLGNICWFTNLDHAKRHEELLLYK----------AY--SEEEYPKYDNYDAIEVNKVKD 255
WP_011967238      199 RYGTEVSVN-SDG----QTIIATNGCLWLTNLELSQRHKDIVLTK----------EYkgNEDKYPHFDNCDGINVNRTQD 263 Parabacter...
tigr:CHAB381_0064 222 PRKTRFRID-ENG----IKWRSMGNICWFTNLDIEKRHEDLLLYE----------VY--NEKKYPKYDNYNAINIDKVAQ 284
jgi:Dehly_1157    264 REFADGVYD-ET-----TGLVKFRNVGWFTNLDHGRRHEKLPLMTmadnlkfskhKEikGKAAYARYDNYDAIEVPFTDA 337
CDD20295          222 YAPTYKFEReMNG----VKVNRVAGICWYTNIEHGRRHHPLTLMTegdnikyskyKEi-KGIGYQHYDNYDAIEVNYFDA 296
CDE57090          222 LNASKCRVD-KSG----RKFIRVKGVRWFTNIDHGRRHEPLALMTmadnlrytkhKElkGKAEYDHYDNYDAIEVPFTDA 296
CCY26553          235 LDAAGCGVDgETG----RKYIRVKGVRWFTNLDIKQRHEEMILVR----------RY--TPDEYPKYVNYDAIEVAKTSD 298
WP_013065369      220 LESNRKFVR-SKGlnpdDNYIKVPAICWYTNLDISKRHETLDLVC----------KY--SPEEFPHYNNFDAIDVGKVQD 286 Prevotella...
WP_006193129      291 IPCDYFENLGVPLTYLDKYNPSQFEIV------------------------------Gssrelaqpmseiapkgsyepgg 340 Selenomona...
WP_004219155      281 IPCDYEGIMGVPVTFMDKFNPDQFEIIgl--------------------------laG---------------------- 312 Bifidobact...
ACL33436          256 IPFDYQGAMGVPITFMDKYNPDQFEILsand------------------------------------------------- 286
WP_011967238      264 IPKDYHGLMGVPITFLHKYNPEQFKIVrf--------------------------rkG---------------------- 295 Parabacter...
tigr:CHAB381_0064 285 IPIDYNGYMGVPITFMHKYNPDQFELIgim-------------------------ntG---------------------- 317
jgi:Dehly_1157    338 IPNDYEGIMGVPITFLDKYNPEQFEIIgl--------------------------aaG---------------------- 369
CDD20295          297 IPSDYQGIMGVSITYLDKYCPEQFEILwq--------------------------acGntrasa---------------- 334
CDE57090          297 IPSDYDGVMGVPVTWLDKYCPEQFEIVgl--------------------------tsGrdefi----------------- 333
CCY26553          299 IPCDYAGAMGVPITFMDKYNPDQFEIVgasqhlireisddirakarysqigcfyldnG---------------------- 356
WP_013065369      287 IPYDYEGVMGVPDTILGQFNPDQFEIIgl--------------------------gsGtlgqsig--------------- 325 Prevotella...
WP_006193129      341 psfylavdrfsdtadnlngterngterngterngtERNGTER-NGTERNGTE-RKYR--RLYFRIVVRR 405 Selenomonas sputigena
WP_004219155      313 -----------------------------------NIRGLAGiPSSTGKDGPyINGK--LKFGRILVRN 344 Bifidobacterium
ACL33436          287 --------------------------------friSNKIPFKeHGLIKDKDGtINGK--PTYVRIVIKH 321
WP_011967238      296 ----------------------------------------------DDGKDLrINGK--CPYFRILVKN 316 Parabacteroides
tigr:CHAB381_0064 318 ------------------------------------EENKGIrHENTSHGRPiINGV--EKYLRVLIRR 348
jgi:Dehly_1157    370 -----------------------------------NIRGLAGiPSATGKDGPyMDGK--LKYGRILIRH 401
CDD20295          335 ------------------------------pkeilKEIGYSV-NPEDRGGCGvVNGK--RQYARILIRK 370
CDE57090          334 -------------------------------arptK--KYIR-PMQVNKNGSlTNGS--KANTRstilm 366
CCY26553          357 ------------------------------------------------------DGTyrKMYDRIVIRN 371
WP_013065369      326 -----------------------------vggipkEHKRLMKgHSAAGDLYYmKNGLpkVPYSRILIRN 365 Prevotella ruminicola
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