2ISJ


Conserved Protein Domain Family
BluB
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cd02145: BluB 
5,6-dimethylbenzimidazole synthase
BluB catalyzes the O2-dependent conversion of FMNH2 to 5,6-dimethylbenzimidazole (DMB), a component of vitamin B12; is is a subfamily of the nitroreductase family; nitroreductases typically reduce their substrates by using NAD(P)H as electron donor and often use FMN as a cofactor.
Links
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Statistics
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PSSM-Id: 380321
Aligned: 11 rows
Threshold Bit Score: 263.448
Created: 19-Jan-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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FMN bindinghomodimer
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:FMN binding site [chemical binding site]
Evidence:
  • Structure:2ISJ: the homodimer of Sinorhizobium meliloti BluB binds two molecules of FMN, contacts at 4A
    - View structure with Cn3D
Download Cn3D for Viewing 3D StructureScroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1               ### #                       #####                                         
2ISJ_A         27 YRAIETRRDVRDEFLPEpLSEELIARLLGAAHQAPSVGFMQPWNFVLVRQdETREKVWQafqrandeaaem-fsgerqak 105  Sinorhizobium...
NP_661830      12 YKVIYSRRDVRGQYLPDpVPEEVLRRVLDAAHHAPSVGFMQPWDFVVVHDlAVRKQIKEgfevahaeaaem-fegekreq 90   Chlorobium te...
NP_769894      13 RELFVWRRDVRRFRTDP-LPDGAVERLIETACLSPSVGLSQPWRFVIVDDaARRTAVIDdfrtcngdalna-yageraar 90   Bradyrhizobiu...
CAB76073      651 LKVMRERRDIRNGFRSDpIPHEVLLRVLEAAHTAPSVGHSQPWDFVVIRSaDTRRAMHElatrqrdayaks-lpkgrakq 729  Streptomyces ...
EAA20272      282 YRVIRERRDMRHFRPDP-VDPAVLRRLLWAAHQAPSVGFMQPWRFIRITDrDLRGRIHDlveterrrtae--algdrade 358  Plasmodium yo...
AAN34221       12 FELIKWRRDVRHFRRDP-VPEDMVQALKTAMDHAPSVGNARPWRIFSVESqAKRKAVHHifteanqaaaqi-ydgarasq 89   Brucella suis...
tigr:SPO3254   15 DLLMRLRRDVRRFRTDP-VDEAVLTRCLDTFRLAPSVGLSEPWRVIRVESdAARAAALEnyqsangealkg-ysgeraqq 92   Silicibacter ...
YP_145300      20 YRALLTRRDIRHFRPDP-IPEEVLERLYQAFHAAPSVGLTQPWAIVEIRErATKERVFAlhqearareral-fqgkaqrv 97   Thermus therm...
YP_001296342    4 DQIIQSRRDTRHFTDDE-VPDQVIEKALQAGHYAPSVGLTNATKYYTIKCkKIKKAIKKifldydqkainltdtqqqkqq 82   Flavobacteriu...
TRA94866       35 YRAIETRRDVRDQFLPDpLPDDLVERLLKAAHSAPSVGFMQPWNFTLVTDgAVRQAAFVafsraneeaaam-ftgekqal 113 
CAA86583       12 TEVLRWRRDVRHFRPDP-IDEAVIDRLRAVMDMAPSVGNARPWRVIRVDSpALRAEVLAnfnaaraaagsa-yageqaea 89   Rhodobacter c...

Feature 1             ##                               #   #                    ####              
2ISJ_A        106 yrsLKLEGIRKAPLSICVTCDRtrggavvlgrthnPQMDLYSTVCAVQNLWLAARAEGVGVGWVSIFhESEIKAILGIPD 185  Sinorhizobium...
NP_661830      91 yrtFKLEGILEAPVGICVTCDRsrsgevvigrtanPEMDLYSSVCAVQNLWLAARAENLGVGWVSIIhHDHVRRVLGIPE 170  Chlorobium te...
NP_769894      91 yatLKLSGLEQAPGHLAVFADKasdtghglgratmPETTEYSVVAAITAMWLAARAEGIGLGWVSILhPDRIHTILDVPD 170  Bradyrhizobiu...
CAB76073      730 fkeLKIEAILDTPVNIVVTADPtrggrhtlgrhtqPQMAPYSSALAVENLWLAARAEGLGVGWVSFFdEREMVRALGLPE 809  Streptomyces ...
EAA20272      359 frkLKIEGIRECGELLAAVLTDgrerhv-fgrrtlPEMDLASLACAIQNLWLAARAEGLGMGWVSFFdPVDLAGMLEIPP 437  Plasmodium yo...
AAN34221       90 yhaLKLEGIETAPVQLAVFTDTapaeghglgrqsmASTLEQSTAMAVQNLNLAARSFGLGAGMVSVLdPQAMEQLFEVPP 169  Brucella suis...
tigr:SPO3254   93 ysqLKLSGMQEAPVQLAVFCDDatakghglgagtmPEMRRYSVVSAITLFWLMLRAEGLGLGWVSILdPDRLCRDLRAPE 172  Silicibacter ...
YP_145300      98 ydrLRLEGLLEAPLHLAVFQRPvarsl---ghhtmPETLAYSVVLAVGNLWTAARAEGIGVGWVSILePEAVARLLGAPP 174  Thermus therm...
YP_001296342   83 yqaLKLEAIEEAPLGLVICYDRsvlnnftigtvgsNEAIKFSAVCAAQNIWLSLTAQGYSMGWVSILnYYEFKKLLGLPE 162  Flavobacteriu...
TRA94866      114 yrsLKLEGIRKAPLSICVTCDPtrggkvvlgrthnPRTDVYSTVCAIQNLWLAARVEGIGVGWVSIFhDSDIRAILDIPE 193 
CAA86583       90 yatLKLQGIDQAPLQLAVFTHRdpaagh-glgrasMPVTLQQSTAMAFTRSGCRAGENLGLGMVSVLdPKAVERLLNAPP 168  Rhodobacter c...

Feature 1                             # #                
2ISJ_A        186 HVEIVAWLCLGFVd-RLYQEPELAAKGWRQRLPLEDLVF 223  Sinorhizobium meliloti
NP_661830     171 HIVPVAWLCIGYVs-FFHEAPELEQAGWLPRLALDDLLH 208  Chlorobium tepidum TLS
NP_769894     171 TWKFIAYLCIGYPe-VECDQPELEQAKWEHRRSANEFTL 208  Bradyrhizobium japonicum USDA 110
CAB76073      810 HLDVVAYLCVGYVd-EFPDEPELMQAGWSKRRPLSWVVH 847  Streptomyces coelicolor A3(2)
EAA20272      438 GGKPVALLCLGHVe-SFYPRPLLESAAWERRRDLEDVVF 475  Plasmodium yoelii yoelii
AAN34221      170 SWRFTLYLCIGWPc-TEDDTPLLHRNGWQENRETFWRRA 207  Brucella suis 1330
tigr:SPO3254  173 TWRLIGYFCIGWPq-ECSDIPELETAGWEVRDRRLTVEI 210  Silicibacter pomeroyi DSS-3
YP_145300     175 DYRLLAYLCLGYPr-AWPDEPLLERAGWRQREPLLRYRE 212  Thermus thermophilus HB8
YP_001296342  163 NIEPLGYFCIGKPatNYNNQPMLQQLNWKQKPEKPSVQE 201  Flavobacterium psychrophilum
TRA94866      194 HIEIVAWLCLGRVd-TLYTEPELAVKGWRQRVPLEELVF 231 
CAA86583      169 DWDFVAWLCIGVPe-FTDDTPLLHRAGWQENLPTEWERR 206  Rhodobacter capsulatus

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