Conserved Protein Domain Family
TFIIA

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pfam03153: TFIIA 
Transcription factor IIA, alpha/beta subunit
Transcription initiation factor IIA (TFIIA) is a heterotrimer, the three subunits being known as alpha, beta, and gamma, in order of molecular weight. The N and C-terminal domains of the gamma subunit are represented in pfam02268 and pfam02751, respectively. This family represents the precursor that yields both the alpha and beta subunits. The TFIIA heterotrimer is an essential general transcription initiation factor for the expression of genes transcribed by RNA polymerase II. Together with TFIID, TFIIA binds to the promoter region; this is the first step in the formation of a pre-initiation complex (PIC). Binding of the rest of the transcription machinery follows this step. After initiation, the PIC does not completely dissociate from the promoter. Some components, including TFIIA, remain attached and re-initiate a subsequent round of transcription.
Statistics
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PSSM-Id: 460829
Aligned: 56 rows
Threshold Bit Score: 158.354
Created: 21-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
EPE35465        9 VYSQIIGDVIESSRVDFEEGGVDEAVLEELKTVWQNKLSSLNVATfpwDPKPEPPQAVVQPptlpsnagnymsapphgv- 87  
EHA98530      710 LYKSIIEDVIEGVRDLFAEEGIEEHVLKDLKQLWETKVLQSKATE---DFFGNGIHSPLFTfqlphtlhqtlqstaslvi 786 
XP_005324530   10 LYRSVIEDVIEGVRDLFVEEGIEEQVLKDLKQLWETKVLQSKATE---DFFRNSVHSPLFTlqlphslhqtlqsstaslv 86  
Q8R4I4         10 LYQSVIEDVIEGVRDLFAEEGIEEQVLKDLKKLWETKVLQSKATE---DFFRNSTQVPLLTlqlphalppalqpeaslli 86   house mouse
DAA24681       10 LYRSVIEDVIEGVRDLFAEEGVEEQVLKDLKQLWETKVLQSKATE---DFFRNSVHSPLFTlqlphslhqtlqssaasfv 86  
XP_011970504   10 LYRSVIEDVIEGVRDLFAEEGVEEQVLKDLKQLWETKVLQSKATE---DFFRNSVHSPLFTlqlphslhqtlqssaaalv 86  
Q9UNN4         10 LYRSVIEDVIEGVRNLFAEEGIEEQVLKDLKQLWETKVLQSKATE---DFFRNSIQSPLFTlqlphslhqtlqsstaslv 86   human
NP_001191795   10 LYKSVIEDVIESIQGLFAEEGVDKQVLRNLKELWETKVMQSKATE---GLFKHSHHSSHLTvqfphnfhhvlqastasfv 86  
XP_010595593   10 LYRSVIEDVIERVRNLFAEEGIEEQVLKDLKQLWETKILQSKATD---DFFRNSIHSPLFTlqlphglhqtlqtsaaplv 86   African savan...
XP_031454381   10 LYKSIIDDVIESIQDLFAEEGIDKQVLRNLKELWETKVMQSKATE---GLFKHRHHSSHLTlqvpqnfhhvlqapaaslv 86   Ring-necked p...
EPE35465       88 ----------qpqmqqmpplqnpngprIKTEP------GLENSNIGMsqapyqNPVLam-----pNATTAQQRAAQHLQN 146 
EHA98530      787 pagi----tfpgfttaelgtsnssasftfpGYpihvpeGVTLQTASG------HLYKvnvpimvtQASRRPSILQHPI-Q 855 
XP_005324530   87 iptg---rtlpsfataelgtsnssanftfpGYpihvpaGVTLQTASG------HLYKvnvpimvtQTSGRASILQHPVQQ 157 
Q8R4I4         87 pagr----tlpsftpedlntancganfafaGYpihvpaGMAFQTASG------HLYKvnvpvmvtQTSGRTEILQHPFQQ 156  house mouse
DAA24681       87 ipag---rtrpsftaaelgtsnssasftfpGYpihvpvGVTQQTASA------HLYKvsvpfmvtQTSERASILQHPVQQ 157 
XP_011970504   87 ipag---rtrpsftaaelgtsnssasftfpGYpihvpvGVTQQTASA------HLYKvsvpfmvtQTSERASILQPPVQQ 157 
Q9UNN4         87 ipag---rtlpsfttaelgtsnssanftfpGYpihvpaGVTLQTVSG------HLYKvnvpimvtETSGRAGILQHPIQQ 157  human
NP_001191795   87 iptgggfqhfmaadlgasqkgatltfpSSVAYpihvpaGLTLQTASG------QLYKvnmpvmvaHGQGDASILQHPLQQ 160 
XP_010595593   87 ippgrtipsfttaelgtsnssanftfpSGIGYpihvpaGVTLRTASG------HLYKvnvpiivtQTPGRASILQHPVQQ 160  African savan...
XP_031454381   87 iptgggfqhfmaadlgasqkgatltlpSSVAYpihvpaGLTLQTASG------QLYKvnmpvmvaHGQGDASILQHPLQQ 160  Ring-necked p...
EPE35465      147 S-----YGQRAAASINAIQGSappQAGQPGMQMTQqqqqaMQQQQHRQMQMqQQQQQAQRggqmppqlqsnrgpaptmtq 221 
EHA98530      856 VfqqlgQPSVIQASVPQLNPs---SVQATTEKSQRme--aMLQQPTILHSG-PVDRKHLEnatrdvfvp----------- 918 
XP_005324530  158 VfqqfgHPSMIQTSVSQLNPCs-lQ---ATNKSQRme--tVLQQPTILHSG-PVDRKLLKnatsdilvp----------- 219 
Q8R4I4        157 VlqqlgQPLVIQTTVPTLHPc---SLQAATEKSLRme--aVLQPPPILHPP-PVDRTHVEnaasd--------------- 215  house mouse
DAA24681      158 VfqllgQPSIIQTSIPQLNPc---FLQATTEKSLRme--tVLQQPIVLPSE-TVDRKHLEnttsdilvp----------- 220 
XP_011970504  158 VfqllgQPSIIQTSIPQLNPc---FLQATTEKSLRme--tTLQQPIVLPSE-TVDRKHLEntgne--------------- 216 
Q9UNN4        158 VfqqlgQPSVIQTSVPQLNPw---SLQATTEKSQRie--tVLQQPAILPSG-PVDRKHLEnatsdilvs----------- 220  human
NP_001191795  161 VfqpigQPSVLKTNLATVAPVrtsSVQATAEILQTqk--tEVQQTVVFQPS-AFHNKHPEnstntvlfqqpa-------- 229 
XP_010595593  161 IfqqlgQPPVIQMSLPQLSs----SPHTTTEKPQGle--pVFQQPTVLHPG-TVDRKHVEnvttdv-------------- 219  African savan...
XP_031454381  161 IfqpigQPSVLKTNVASVAPVrtsSVQATAETLQTqk--tEVQQTVVFQPS-TLQNKHPEnstnivlfqqpa-------- 229  Ring-necked p...
EPE35465      222 eqyrqamaaqaqqKLQSQG------VVngqnpqmpnvqngqgqsSLaGAQVDGGDDEVES--------VGLVKQLNENGE 287 
EHA98530      919 -----pgnehgvlSEALLS------QQegsqcvhv--sgvvfpsQA-SPTGSSVEPVLSVparvtqnlLGGCLSAETQGT 984 
XP_005324530  220 -----pgnehkimPEALLR------QQensqyisl--pgvvfppQI-LQTDSNVDPVLSVsasmtqnlHSGPLSLGPQVS 285 
Q8R4I4        216 ---------rrllPGNELR------PQesspylsl--pgvgfppQA-ALTESSLEPVLGVsasltqnlHSDPFSQGPPGP 277  house mouse
DAA24681      221 -----pgnehktiSEALLN------QLdsssemdlsi------------------------------------------- 246 
XP_011970504  217 ---------hkiiSGALLN------QLvssqyisf--pgvvftpQA-SQAGSNVEPVLRVsarmtqnlHG-PLSTSPQGA 277 
Q9UNN4        221 -----pgnehkivPEALLC------HQesshyisl--pgvvfspQV-SQTNSNVESVLSGsasmaqnlHDESLSTSPHGA 286  human
NP_001191795  230 --vsqqeiatnavLNQCAE------LTeksqysnl--htavftsGS-SEVFSPAESLANDpgsvllpdVEGQLDMKPQES 298 
XP_010595593  220 --------fvppgNEHKIMldvslsQQespqyvnl--pgvvfapQV-SQLDSNVESALSVsttvtqnlHGRPYSAGPRGA 288  African savan...
XP_031454381  230 --vsqqeiatnavLNQCAE------LTeksqysnv--htavftsGS-SVVFSPAESLANDpgsvllpdVEGQLDMKPQES 298  Ring-necked p...
EPE35465      288 et-imGRFEIDGMirqkieamgqkmeggglmlplkqaSTAS---KRQRKVKKSEL------------------------- 338 
EHA98530      985 vhhqvTDTQPH-F------------------------LKYCvygGDSISQPGEIEepnslpvskknsnsqvdlsnlltdd 1039
XP_005324530  286 fh-hmTDAQLH-V------------------------LKKRmygCDSVKQPRKIEepsslpvseknsnsqmdlntqvsdd 339 
Q8R4I4        278 lhhhlLESQLQ-S------------------------LKDSiygCDSTKQLRKAEepsslrvseknctserdlnirvtdd 332  house mouse
DAA24681      247 ---------------------------------------------------------------------------rvtdg 251 
XP_011970504  278 qqqhvPEAQPR-V------------------------LKNKmcgCDSVKQPRNTEepcslpvsekdsssqmdlsiqvtdg 332 
Q9UNN4        287 lhqhvTDIQLH-I------------------------LKNRmygCDSVKQPRNIEepsnipvsekdsnsqvdlsirvtdd 341  human
NP_001191795  299 vqqqvSGGIIELI------------------------IKGKsldGNAALKDQDSEaptdkmepteqves-dlqsekgics 353 
XP_010595593  289 lhqhlPDVQLR-V------------------------LKNRvygCDSVKQPRNVQepsslpvsekvhrnknkwkfylkdg 343  African savan...
XP_031454381  299 vqqqvSDDIIELI------------------------IKGKsldDNTALKDQDSEaptdkmepteqves-dlqseggics 353  Ring-necked p...
EPE35465      339 ----SGQHDGAGSDDDLke-------------------------------------eDID---------------EDAIN 362 
EHA98530     1040 dineIIQVDGTGDTSSNkeiknirdvkneflgii------dggdlkvleevdsisneDSTtnssdnedsqldiveEDPLN 1113
XP_005324530  340 dineIIQIDGTGDTSSNeeiesirdvnenefigii-----dagdlkvleeagsisneDSPanssddedaqidvveEDPLN 414 
Q8R4I4        333 dineIIQIDGTGDNSSTeemgsirdadenefpgii-----dagdlnvleevdsvsneDSTanssdnedhqinapeEDPLN 407  house mouse
DAA24681      252 dvneIIQIGGTGETSSNdeigntrdvdengllgii----dagdlkvteeetgsvsneDSSvnssdnedpeidlleEDPLN 327 
XP_011970504  333 diheIIQIGGTGDTSSNdeigntrdvdengllgii----gagdlkvpeeetgsvsneDSSvdssdnedpeidlveEDPLN 408 
Q9UNN4        342 digeIIQVDGSGDTSSNeeigstrdadeneflgni----dggdlkvpeeeadsisneDSAtnssdnedpqvniveEDPLN 417  human
NP_001191795  354 dtegIIQLDGTCDGSPKkevlhtkdteenefidiiesedlkiledeedeeadsisnsESSsscdnee-pqtdvveEDPLN 432 
XP_010595593  344 vmcfggkdyvfakavgdaew------------------------------------------------------------ 363  African savan...
XP_031454381  354 dtegIIQLDGTCDGSPKkeglhtkdteenefidiiesedlkilegeedeeadsisnsESSsscdnee-pqtdiveEDPLN 432  Ring-necked p...
EPE35465      363 SDlDDPDDGLNEE-DDDEDsnshIMLCMYDKVQRVKNKWKCVMKDGVLTVNGKEYVFHKAQGEYEW 427 
EHA98530     1114 SG-DDVSEQDVPDlFDTDN----VIVCQYDKIHRNKNKWKFYLKDGIMCFGGRDYVFAKAIGDAEW 1174
XP_005324530  415 SG-DDVSEQDVPDlFDTDN----VIVCQYDKIHRSKNKWKFFLKDGVMCFGGRDYVFAKAIGDAEW 475 
Q8R4I4        408 SG-DDVSEQDVPDlFDTEN----VIVCQYDKIHRSKNRWKFYLKDGVMCFGGRDYVFAKAIGEAEW 468  house mouse
DAA24681      328 SG-DDVSEQDVPDlFDMDN----VIVCQYDKIHRSKDKWKFYLKDGVMCFGGRDYVFAKAIGDAEW 388 
XP_011970504  409 SG-DDVSEQDVPDlFDMDN----VIVCQYDKIHRSKDKWKFYLKDGVMCFGGRDYVFAKAIGDAEW 469 
Q9UNN4        418 SG-DDVSEQDVPDlFDTDN----VIVCQYDKIHRSKNKWKFYLKDGVMCFGGRDYVFAKAIGDAEW 478  human
NP_001191795  433 SD-DDVSDLDIADlFDTNN----VIVCQYEKIHRSKNKWKFFLKDGVMSFNGKDYVFAKAVGDAEW 493 
XP_010595593      ------------------------------------------------------------------      African savanna elephant
XP_031454381  433 SD-DDVSDLDIADlFDTNN----VIVCQYEKIHRSKNKWKFFLKDGVMSFDGKDYVFAKAVGDAEW 493  Ring-necked pheasant
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