5JRH,1RY2,2P2B,2P2F,1PG3,5IFI


Conserved Protein Domain Family
ACS

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cd05966: ACS 
Click on image for an interactive view with Cn3D
Acetyl-CoA synthetase (also known as acetate-CoA ligase and acetyl-activating enzyme)
Acetyl-CoA synthetase (ACS, EC 6.2.1.1, acetate#CoA ligase or acetate:CoA ligase (AMP-forming)) catalyzes the formation of acetyl-CoA from acetate, CoA, and ATP. Synthesis of acetyl-CoA is carried out in a two-step reaction. In the first step, the enzyme catalyzes the synthesis of acetyl-AMP intermediate from acetate and ATP. In the second step, acetyl-AMP reacts with CoA to produce acetyl-CoA. This enzyme is widely present in all living organisms. The activity of this enzyme is crucial for maintaining the required levels of acetyl-CoA, a key intermediate in many important biosynthetic and catabolic processes. Acetyl-CoA is used in the biosynthesis of glucose, fatty acids, and cholesterol. It can also be used in the production of energy in the citric acid cycle. Eukaryotes typically have two isoforms of acetyl-CoA synthetase, a cytosolic form involved in biosynthetic processes and a mitochondrial form primarily involved in energy generation.
Statistics
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PSSM-Id: 341270
Aligned: 42 rows
Threshold Bit Score: 961.634
Created: 10-Dec-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 35 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1PG3_A; Salmonella enterica Acetyl CoA synthetase binds AMP-propyl ester and CoA, contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
5JRH_A        22 EQYETKYKQSinDPDTFWGEQGKi-LDWITPYQKVkntsfapgnvSIKWYEDGTLNLAANCLDRHLQE-NGDRTAIIWEG 99  Salmonella ente...
1PG3_A        22 EQYETKYKQSinDPDTFWGEQGKi-LDWITPYQKVkntsfapgnvSIKWYEDGTLNLAANCLDRHLQE-NGDRTAIIWEG 99  Salmonella ente...
P27095        48 EKEMRAWCSSdeHYLEFWDEMAKtyVDWHKPYTKVmdds---empYFHWFTGGEINITYNAVDRHAKGaKKDKVAYIWIP 124 Methanosaeta co...
Q55404        31 REYEQLYSRAasNPEKFWGELAEqeLHWFKKWDQVldw----qppFAKWFVGGQLNISHNCLDRHLTTwRRNKAAIIWEG 106 Synechocystis s...
O93730        31 DAYFKFHRQTveNLESFWESVAKe-LEWFKPWDKVldas---nppFYKWFVGGRLNLSYLAVDRHVKTwRKNKLAIEWEG 106 Pyrobaculum aer...
Q9RRL7        33 AEYERRYRQSldQPDDFWSEVAHd-LHWMKDWDRVldw----qepHAQWFVGGQTNIAYNALDRNVQRgLGDKRAIIWEG 107 Deinococcus rad...
YP_445803     30 VSDPAIYDRFd-DFPEGFEEYADl-LDWDTRWDEVfdgs---dppFFEWFTGGELNACYNCVDRHLHE-RPNQAAFIWEG 103 Salinibacter ru...
YP_001633650  38 NSYDELYQWTisHREEFWADMASq-LEWFKPWDKVldds---nkpFYQWFVGGKTNIVYNAIDRHLKTwRKNKLALIWEG 113 Chloroflexus au...
C1AA44        26 VRDNALREAAaaDPVGFWAKESEa-LDWMTPWSTAlew----eppRAKWFQGGTLNASVNCIDRHVHGpRRNKAALIWEG 100 Gemmatimonas au...
P9WQD0        23 NARAELYREAeeDRLAFWAKQANr-LSWTTPFTEVldws---gapFAKWFVGGELNVAYNCVDRHVEAgHGDRVAIHWEG 98  Mycobacterium t...
Feature 1                                                                              ###       
5JRH_A       100 DDTsqs------khISYRELHRDVCRFANTLLDLGi-kKGDVVAIYMPmVPEAAVAMLACARIGAVHSVIFGGFSpEAVA 172 Salmonella ente...
1PG3_A       100 DDTsqs------khISYRELHRDVCRFANTLLDLGi-kKGDVVAIYMPmVPEAAVAMLACARIGAVHSVIFGGFSpEAVA 172 Salmonella ente...
P27095       125 EPTdqp-----vqkITYGDLYKEVNKFANGLKSLGl-kKGDRVSIYMPmIPQLPIAMLACAKLGVSHIVVFSGFSsKGLM 198 Methanosaeta co...
Q55404       107 EPGds-------riITYAQLHREVCQFANALKSLGv-qKGDRVAIYLPmIPEAAITMLACSRIGAPHSVVFGGFSaEALR 178 Synechocystis s...
O93730       107 EPVdengyptdrrkLTYYDLYREVNRVAYMLKQNFgvkKGDKITLYLPmVPELPITMLAAWRIGAITSVVFSGFSaDALA 186 Pyrobaculum aer...
Q9RRL7       108 EDGev-------rtYTYAELLREVCKAANALEELGv-vAGDRVTLYMPlIPEAAIAMLACARIGAVHSIVFGGFSvSALA 179 Deinococcus rad...
YP_445803    104 EDGtr-------rtLTYRDLYREVNKMAASLRDVGv-qEDDVVTLHLPmVPALPITMLACARIGAPHSVVFGGFSaSALA 175 Salinibacter ru...
YP_001633650 114 EDGsq-------rtYSYYQLNYEVSRIANVLKSMGv-kKGDIVTIYMPrIPELMFSMLACAKIGAAHSVVYGGFSeAALA 185 Chloroflexus au...
C1AA44       101 EPGdr-------rtFTYWDLYREVNLAANMLKKLGv-gRGDRVAIYLPmIPEAVIAMLACARIGAIHTVVFGGFApESLR 172 Gemmatimonas au...
P9WQD0        99 EPVgdr------rtLTYSDLLAEVSKAANALTDLGl-vAGDRVAIYLPlIPEAVIAMLACARLGIMHSVVFGGFTaAALQ 171 Mycobacterium t...
Feature 1                          #  # #                                                        
5JRH_A       173 GRIIDssSRLVITADEGVRAGRSIPLKkNVDDALknpnvtsVEHVIVLKrtgsd------idwqeGRDLWWRDLIEkasp 246 Salmonella ente...
1PG3_A       173 GCIIDssSRLVITADEGVRAGRSIPLKkNVDDALknpnvtsVEHVIVLKrtgsd------idwqeGRDLWWRDLIEkasp 246 Salmonella ente...
P27095       199 DRAAHcgSRAIITVDGFYRRGKPVPLKpNADEAAgga--psVEKIIVYKragvd------vsmkeGRDVWWHDLVKgqse 270 Methanosaeta co...
Q55404       179 DRLVDaeAKLVITADGGFRKDKAIALKqEVDKALehg-apsVENVIVVQrtkad------vtmtaGRDHWWHELQPqqsa 251 Synechocystis s...
O93730       187 ERINDsqSRIVITADGFWRRGRVVRLKeVVDAALeka--tgVESVIVLPrlglkd-----vpmteGRDYWWNKLMQgipp 259 Pyrobaculum aer...
Q9RRL7       180 DRINNaqSKLLITADAGYRRGKPVTLKiNADEAAkla--pcLEHVLVVKragipl-----ewwteGRDLWWHDVVDrqsd 252 Deinococcus rad...
YP_445803    176 QRATDadSDVIVTIDGYYRRGEFLHHKeKADTAVeea-dtdIDTVLVWErhegtlh--peadleeGRDILVSELLAnner 252 Salinibacter ru...
YP_001633650 186 DRLADakSKVLITADGGYMRGKVVELKkIVNEALsrt--ptVQTCLVFRhtnhg------apmeqGRDFWMHDLLGlpia 257 Chloroflexus au...
C1AA44       173 DRINDcgCKLLITADGGSRRGQMVPLKrNADVALkec--psIENVLVVMrrrsgvgdetfaemqeGRDHWWHRLKRqvpr 250 Gemmatimonas au...
P9WQD0       172 ARIVDaqAKLLITADGQFRRGKPSPLKaAADEALaaipdcsVEHVLVVRrtgie------mawseGRDLWWHHVVGsasp 245 Mycobacterium t...
Feature 1                                                                     #    ##            
5JRH_A       247 --ehqPEAMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAATTFKYVFDYHP-GDIYWCTADVGWVTGHSYLLYGPLAc 323 Salmonella ente...
1PG3_A       247 --ehqPEAMNAEDPLFILYTSGSTGKPKGVLHTTGGYLVYAATTFKYVFDYHP-GDIYWCTADVGWVTGHSYLLYGPLAc 323 Salmonella ente...
P27095       271 --ecePVWVDPEHRLYILYTSGTTGKPKGIEHATGGNAVGPAQTLHWVFDLKD-DDVWWCTADIGWVTGHSYIVYAPLIl 347 Methanosaeta co...
Q55404       252 --hcpAEPIDSEDMLFILYTSGSTGKPKGVVHTTGGYNLYTHMTTKWIFDLKD-TDVYWCTADVGWITGHSYIVYGPLSn 328 Synechocystis s...
O93730       260 nayiePEPVESEHPSFILYTSGTTGKPKGIVHDTGGWAVHVYATMKWVFDIRD-DDIFWCTADIGWVTGHSYVVLGPLLm 338 Pyrobaculum aer...
Q9RRL7       253 --qheATALDSEHPLFILYTSGSTGAPKGVQHTTGGYMVGTYLTTQTVFDLRD-DDIYWCTADIGWITGHSYSVYGPLLn 329 Deinococcus rad...
YP_445803    253 -arvePVSRDAEDTLFLMYTSGTTGKPKGAQHRTGGYLSYVAGTSKYVLDIKP-NDTYWCAADIGWITGHSYIVYGPLAl 330 Salinibacter ru...
YP_001633650 258 nghcpTEEMDAEDMLFILYTSGTTGKPKGVVHTHGGYMVGTYTTLKFVFDIKD-EDRYWCAADPGWITGHSFIVYAPLIn 336 Chloroflexus au...
C1AA44       251 --ycePEAMDAEDVLFVLYTSGTTGKPKGIVHTTGGFLTGVATTTKYTFDLKE-EDVYWCTADIGWITGHSYLVYGPLAn 327 Gemmatimonas au...
P9WQD0       246 --ahtPEPFDSEHPLFLLYTSGTTGKPKGIMHTSGGYLTQCCYTMRTIFDVKPdSDVFWCTADIGWVTGHTYGVYGPLCn 323 Mycobacterium t...
Feature 1                 ###                       ##  #                      ####              
5JRH_A       324 GATTLMFEGVPNWPTPARMCQVVDKHQVNILYTAPTAIRALMAEGDKaieGTDRSSLRILGSVGEPINPEAWEWYWKkIG 403 Salmonella ente...
1PG3_A       324 GATTLMFEGVPNWPTPARMCQVVDKHQVNILYTAPTAIRALMAEGDKaieGTDRSSLRILGSVGEPINPEAWEWYWKkIG 403 Salmonella ente...
P27095       348 GMTSLMYEGAADYPDFGRWWKNIQDHKVTVLYTAPTAVRMFMKQGAEwpdKYDLSSLRLLGSVGEPINPEAWMWYREhIG 427 Methanosaeta co...
Q55404       329 GATTVMYEGVPRPSNPGCFWDVIERYGVNIFYTAPTAIRAFIRMGEAvpnARDLSSLRLLGTVGEPINPEAWMWYHRvIG 408 Synechocystis s...
O93730       339 GATEVIYEGAPDYPQPDRWWSIIERYGVTIFYTSPTAIRMFMRYGEEwprKHDLSTLRIIHSVGEPINPEAWRWAYRvLG 418 Pyrobaculum aer...
Q9RRL7       330 GATVVMYEGAPNQPDWGRFWDIVQKHRVTILYTAPTAIRSFMQHGDEipgRYDLASLRLLGSVGEPINPEAWMWYYRvIG 409 Deinococcus rad...
YP_445803    331 GTTSVMREGAPDHPHKGVTWEIAERHDVDIFHTSPTAVRMYMKWGKEh-pASYDFNFRHMTTVGEPIQPEAWLWYYThIG 409 Salinibacter ru...
YP_001633650 337 GATSFMYEGAPNYPYPDRWWSMVAKHGITILYTAPTAIRGLMRFGDLwpsRHDLSTLRLLGSVGEPINPEAWKWFYEkIG 416 Chloroflexus au...
C1AA44       328 GATCVMYEGAPDWPDKDRFWQICERYGVTILYTAPTAIRAFMKWGTEyvkKHDLSQLRVLGSVGEPINPEAWMWYHEhIG 407 Gemmatimonas au...
P9WQD0       324 GVTEVLYEGTPDTPDRHRHFQIIEKYGVTIYYTAPTLIRMFMKWGREipdSHDLSSLRLLGSVGEPINPEAWRWYRDvIG 403 Mycobacterium t...
Feature 1               ######                                                                   
5JRH_A       404 KEKCPVVDTWWQTETGGfMITPLPGa--iELKAGSATRPFFGVQPALVDNeghpq------egatEGNLVITDSWPGQAR 475 Salmonella ente...
1PG3_A       404 KEKCPVVDTWWQTETGGfMITPLPGa--iELKAGSATRPFFGVQPALVDNeghpq------egatEGNLVITDSWPGQAR 475 Salmonella ente...
P27095       428 RGELQIMDTWWQTETGTfLNSPLPIt---PLKPGSCTFPLPGYDISILDEegnev------plgsGGNIVALKPYPSMLR 498 Methanosaeta co...
Q55404       409 GGKCPIVDTWWQTETGGiMLTPLPGa--iPTKPGSCTKPFPGIVAEIVDLdgnpv------esdqGGFLVIKQPWPSMIR 480 Synechocystis s...
O93730       419 NEKVAFGSTWWMTETGGiVISHAPGlylvPMKPGTNGPPLPGFEVDVVDEngnpa------ppgvKGYLVIKKPWPGMLH 492 Pyrobaculum aer...
Q9RRL7       410 GERCPVVDTWWQTETGSiMLTTLPGa--fPSKPGSAGLPMFGVEPALMTRdgeei------gdddGGLLVIKRPWPSMLR 481 Deinococcus rad...
YP_445803    410 NEDAVIVDTWWQTETGGhLITNLPAl--qDMKPGSAGRPCPGIQPAIYDNngtpve----aasgqAGNLVIERPWPGMLQ 483 Salinibacter ru...
YP_001633650 417 HGRCPIMDTWWQTETGHfMITPTPAv---PLKPGSATRPFLGIEADVVHEdgspc------apdeDGLLVIKNPWPGMMR 487 Chloroflexus au...
C1AA44       408 DFQCPIVDTWWQTETGAiMITPLPGv--tTTKPGSATVPFPGIRTALLDAnane-------ltvgGGLLAITHPWPSMLR 478 Gemmatimonas au...
P9WQD0       404 GGRTPLVDTWWQTETGSaMISPLPGi--aAAKPGSAMTPLPGISAKIVDDhgdplpphtegaqhvTGYLVLDQPWPSMLR 481 Mycobacterium t...
Feature 1                                         #           #  #       ####                    
5JRH_A       476 TLFGDHERFEqTYFSTFk---------NMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAA 546 Salmonella ente...
1PG3_A       476 TLFGDHERFEqTYFSTFk---------NMYFSGDGARRDEDGYYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAA 546 Salmonella ente...
P27095       499 AFWGDKERFMkEYWQFYwdvp---grrGVYLAGDKAQRDKDGYFFIQGRIDDVLSVAGHRIANAEVESALVAHPKIAEAA 575 Methanosaeta co...
Q55404       481 DVYGDTDRFRhTYWEHIqpk----egqYLYFAGDGARRDKDGYFWVMGRVDDVINVSGHRLGTMEIESALVSHPLVAEAA 556 Synechocystis s...
O93730       493 GIWGDPERYIkTYWSRFp---------GMFYAGDYAIKDKDGYIWVLGRADEVIKVAGHRLGTYELESALISHPAVAESA 563 Pyrobaculum aer...
Q9RRL7       482 TVYGDDERYRkSYWGEIp---------HVYFAGDGARRDHDGYYTIVGRVDDVLNVSGHRLGTMEIESALVAHPDVSEAA 552 Deinococcus rad...
YP_445803    484 TVYGDDQRFInEYWRRFsdvdsddwrdWVYEAGDGAVHAQDGYFRVLGRLDDVMNVAGHRLGTMELESAVAQVSEVAEAA 563 Salinibacter ru...
YP_001633650 488 TILNDPQRYVeGYWQKVp---------PYYAAGDSARKDKDGYIWVIGRLDDVIKVSGYRLGTAEVESALVSHPAVAEAA 558 Chloroflexus au...
C1AA44       479 TIWGDDQRYVdTYFSKWpg------rpDLYFPGDGAKLDEDGYLWILGRVDDVLNVSGHRIGTMEVESALVDHPSVAEAA 552 Gemmatimonas au...
P9WQD0       482 GIWGDPARYWhSYWSKFsd-------kGYYFAGDGARIDPDGAIWVLGRIDDVMNVSGHRISTAEVESALVAHSGVAEAA 554 Mycobacterium t...
Feature 1                                             #    ##                                    
5JRH_A       547 VVGIPHAIKGQAIYAYVTLNHGeepspELYAEVRNWVRKEIGPLATPDVLHWTDsLPKTRSGKIMRRILRKIAagdtsNL 626 Salmonella ente...
1PG3_A       547 VVGIPHAIKGQAIYAYVTLNHGeepspELYAEVRNWVRKEIGPLATPDVLHWTDsLPKTRSGKIMRRILRKIAagdtsNL 626 Salmonella ente...
P27095       576 VVGKPDEVKGESIVAFVILRVGnepspELAKDAIAFVRKTLGPVAAPTEVHFVNdLPKTRSGKIMRRVVKARAlg--nPV 653 Methanosaeta co...
Q55404       557 VVGRPDELTGEAIFAFVSLEGNaepseELKKDLVKHVTEEIGAIARPAEIRFTDvLPKTRSGKIMRRLLRSLAsg-qeIS 635 Synechocystis s...
O93730       564 VVGVPDAIKGEVPIAFVVLKQGvapsdELRKELREHVRRTIGPIAEPAQIFFVTkLPKTRSGKIMRRLLKAVAtg--aPL 641 Pyrobaculum aer...
Q9RRL7       553 VVGRPDPVKGESVVAYVLLQDGh---tADPAALRAHVSSEIGALARPDAIYIADaLPKTRSGKIMRRFLRQLAag-qpVQ 628 Deinococcus rad...
YP_445803    564 VAARQDDQKGNVPDVYVTPRDGvaasdELRQDIVAAVEKEIGAFARPGNVIFVGdLPKTRSGKIMRRLLENISng--kDL 641 Salinibacter ru...
YP_001633650 559 AIGLPHEVKGNAIHAFVILRAGyepshDLEEKLRAHVGHELGPIARPDSITFVTsLPKTRSGKIMRRVLRARAlg--lPE 636 Chloroflexus au...
C1AA44       553 VVGKHHDLKGQAIAAFVTLRAGftasgSLRDELRDHVAQKIGALARPDDILFSAdLPKTRSGKIMRRLLRDIAeg--rAL 630 Gemmatimonas au...
P9WQD0       555 VVGVTDETTTQAICAFVVLRANyaphdRTAEELRTEVARVISPIARPRDVHVVPeLPKTRSGKIMRRLLRDVAen--rEL 632 Mycobacterium t...
Feature 1                
5JRH_A       627 GDTSTLAD 634 Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
1PG3_A       627 GDTSTLAD 634 Salmonella enterica
P27095       654 GDISTLMN 661 Methanosaeta concilii
Q55404       636 GDTSTLED 643 Synechocystis sp. PCC 6803
O93730       642 GDVTTLED 649 Pyrobaculum aerophilum str. IM2
Q9RRL7       629 GDTSTLED 636 Deinococcus radiodurans R1
YP_445803    642 GDTTTLRD 649 Salinibacter ruber DSM 13855
YP_001633650 637 GDVSTLEE 644 Chloroflexus aurantiacus J-10-fl
C1AA44       631 GDTTTLAD 638 Gemmatimonas aurantiaca T-27
P9WQD0       633 GDTSTLLD 640 Mycobacterium tuberculosis H37Rv

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