2CN5,2W0J,3I6U


Conserved Protein Domain Family
STKc_Chk2

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cd14084: STKc_Chk2 
Click on image for an interactive view with Cn3D
Catalytic domain of the Serine/Threonine kinase, Cell cycle Checkpoint Kinase 2
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. Checkpoint Kinase 2 (Chk2) plays an important role in cellular responses to DNA double-strand breaks and related lesions. It is phosphorylated and activated by ATM kinase, resulting in its dissociation from sites of damage to phosphorylate downstream targets such as BRCA1, p53, cell cycle transcription factor E2F1, the promyelocytic leukemia protein (PML) involved in apoptosis, and CDC25 phosphatases, among others. Mutations in Chk2 is linked to a variety of cancers including familial breast cancer, myelodysplastic syndromes, prostate cancer, lung cancer, and osteosarcomas. Chk2 contains an N-terminal SQ/TQ cluster domain (SCD), a central forkhead-associated (FHA) domain, and a C-terminal catalytic kinase domain. The Chk2 subfamily is part of a larger superfamily that includes the catalytic domains of other protein kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 270986
Aligned: 10 rows
Threshold Bit Score: 451.847
Created: 26-Jul-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:2CN5: Human Chk2 binds Mg-ADP; contacts at 4A.
  • Structure:2W0J: Human Chk2 binds inhibitor Nsc109555; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                      ####### #            # #                                     #    
2CN5_A        11 PKALRDEYIMSKt-LGSGACGEVKLAFERktCKKVAIKIISKRKFAIGsare-adpALNVETEIEILKKLnHPCIIKIKN 88  human
2W0J_A         5 PKALRDEYIMSKt-LGSGACGEVKLAFERktCKKVAIKIISKRKFAIGsare-adpALNVETEIEILKKLnHPCIIKIKN 82  human
3I6U_A       130 PKALRDEYIMSKt-LGSGACGEVKLAFERktCKKVAIRIISKRKFAIGsare-adpALNVETEIEILKKLnHPCIIKIKN 207 human
XP_972529    142 PQELRLKYTTTKl-LGRGSFGQVRLALDKqtREQFAIKRIDKSRSSVStrr--gnnISGIKTEVEILSSFsHAFIIQMRE 218 red flour beetle
Q9U1Y5       162 PEELTKKYHVTShsLGKGGFGKVLLGYKKsdRSVVAIKQLNTQFSTRCsra--iakTRDIRNEVEVMKKLsHPNIVAIYD 239 nematode
AAH44519     186 PLEFSKKYHIARk-IGTGVCGEVKLAIEKetFKKVALKTINKHDFPSIgt-----aTRNAEREIEILKKIdHPCLIKTED 259 zebrafish
BAD74191     166 PKEISPKYYISRv-LGQGACGLVKLVYDKvkCTKHAMKIIKKSRLTNGqinh-inkPAKIMNEINIMKALrHPCIISIEE 243 domestic silkworm
1848279      150 PEEINKTYYVNRk-LGSGAYGLVRLVYDTrtCQQFAMKIVKKNMLSGArpstnfsdPDRVLNEAKIMKNLsHPCVVRMHD 228 fruit fly
AAF75829     185 PKDFIDKYIMSRp-IGSGACGEVKLAFQKsvCKKVAVKIISKRKFKMNtss--nehPISVDTEIEILKKLdHPCIIKIEN 261 African clawed ...
NP_001073576 190 PREFREKYIMSKt-LGSGACGEVKLAFEKstCNKVAVKIINKRKFMASggre-anpAFNINTEIEILKKIdHPCLIKIKN 267 chicken
Feature 1                   ####   #                                              ## #           
2CN5_A        89 FFDAe-DYYIVLELMEGGELFDKVVGn----KRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQeEDCLIK 163 human
2W0J_A        83 FFDAe-DYYIVLELMEGGELFDKVVGn----KRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQeEDCLIK 157 human
3I6U_A       208 FFDAe-DYYIVLELMEGGELFDKVVGn----KRLKEATCKLYFYQMLLAVQYLHENGIIHRDLKPENVLLSSQeEDCLIK 282 human
XP_972529    219 VFETddEVFLVLEYMSGGELTSRIRSt----LAFPECEVKFIFYQILLGVRYLHLNGVTHRDLKPENVLLSTQnQYPRVK 294 red flour beetle
Q9U1Y5       240 WITVakYSYMVIEYVGGGEFFSKVVDskynrMGLGESLGKYFAFQLIDAILYLHSVGICHRDIKPENILCSDKaERCILK 319 nematode
AAH44519     260 FYQTedSYYIVLEYIEGGELFGRIKAk----KKLEEDIAKLYFYQMLKAVEYLHNNGIIHRDLKPENVLLASHdDICLIK 335 zebrafish
BAD74191     244 VFDSrdAVYIILELMEGGELFDRITKf----SHLTEPLTRFIFRQMVLAVKYLHSQGITHRDLKPENVLLEGDeDETLVK 319 domestic silkworm
1848279      229 IVDKpdSVYMVLEFMRGGDLLNRIISn----KLLSEDISKLYFYQMCHAVKYLHDRGITHRDLKPDNVLLETNdEETLLK 304 fruit fly
AAF75829     262 FFDSedFYYIVLELMEGGELFDRVVNs----TRLREPIAKLYFYQMLLAVQYLHENGVIHRDLKPENVLLSSTsEECCIK 337 African clawed ...
NP_001073576 268 FFEAe-DYYIVLELMEGGELYDRVSRp----VKMKEATCKLYFYQMLLAVKYLHDNGIIHRDLKPENVLLSSSeETCLIK 342 chicken
Feature 1          #                                                                             
2CN5_A       164 ITDFGHSKILGEtslMRTLCGTPTYLAPEVLVSvgtaGYNRAVDCWSLGVILFICLSGYPPFSEHrtqVSLKDQITSGKY 243 human
2W0J_A       158 ITDFGHSKILGEtslMRTLCGTPTYLAPEVLVSvgtaGYNRAVDCWSLGVILFICLSGYPPFSEHrtqVSLKDQITSGKY 237 human
3I6U_A       283 ITDFGHSKILGEtslMRTLCGTPTYLAPEVLVSvgtaGYNRAVDCWSLGVILFICLSGYPPFSEHrtqVSLKDQITSGKY 362 human
XP_972529    295 ISDFGLSKIMDDisvMETVCGTVQYAAPEVLRFf--ePYSRKVDVWSLGVILFYMLSRTQPFVGSs-rEEVAKNIVSGKI 371 red flour beetle
Q9U1Y5       320 LTDFGMAKNSVNr--MKTRCGTPSYNAPEIVANeg-vEYTPKVDIWSLGCVLFITFSGYPPFSEEytdMTMDEQVLTGRL 396 nematode
AAH44519     336 ITDFNQSKILEEsslMKTLCGTPTYVAPEVFTHastvGYTKAVDYWSLGVLLFICLGGYPPFNTEcttMSVREQIINGEY 415 zebrafish
BAD74191     320 ITDFGLSKFVGEdsfMKTMCGTPLYLAPEVLRAngqnTYGPEVDVWSLGVIFFVCLVGYLPFSHDykeMSLKNQILNGQY 399 domestic silkworm
1848279      305 VSDFGLSKFVQKdsiMRTLCGTPLYVAPEVLITggreAYTKKVDIWSLGVVLFTCLSGTLPFSDEy-gTPAAQQIKKGRF 383 fruit fly
AAF75829     338 ITDFGQSKILGEtslMRTLCGTPTYLAPEVLNTagttGYSSAVDCWSLGVILFVCLCGYPPFSEQnsnIPLKNQIAEGKY 417 African clawed ...
NP_001073576 343 ITDFGQSKILGEtslMKTLCGTPTYLAPEVLNSfgtaGYSRAVDCWSLGVILFVCLCGYPPFNEQntqLSLKDQITRGEY 422 chicken
Feature 1                                                 
2CN5_A       244 NFIPEVwAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWL 284 human
2W0J_A       238 NFIPEVwAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWL 278 human
3I6U_A       363 NFIPEVwAEVSEKALDLVKKLLVVDPKARFTTEEALRHPWL 403 human
XP_972529    372 TWDPKAwRNVSLPAKNLVKSMITVDSTCRYSIVDIFKHDWV 412 red flour beetle
Q9U1Y5       397 IFHAQW-RRITVETQNMIKWMLTVEPSNRPSAVELMSTQWM 436 nematode
AAH44519     416 RFIPSQwKNVSNEAKDLVKKLLVVDPQKRLSVEEALEHPWL 456 zebrafish
BAD74191     400 KFSQAHwKNISLQAKLLMKRMLTVQVSRRITLDQILNHAWM 440 domestic silkworm
1848279      384 AYGHPSwKSVSQRAKLLINQMLIVDPERRPSIDDVLQSSWL 424 fruit fly
AAF75829     418 TYIAAAwRNVSEQAFDLVKNLLVVDPEQRLTTKQALEHPWL 458 African clawed frog
NP_001073576 423 TFISKEwKHVSNMALDLVKKLLVVDPSKRFTIEEALEHPWL 463 chicken

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