1JP3,1F75,4H8E,2VG3,2D2R,3UGS,2VG1


Conserved Protein Domain Family
Cis_IPPS

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cd00475: Cis_IPPS 
Click on image for an interactive view with Cn3D
Cis (Z)-Isoprenyl Diphosphate Synthases
Cis (Z)-Isoprenyl Diphosphate Synthases (cis-IPPS) catalyze the successive 1'-4 condensation of the isopentenyl diphosphate (IPP) molecule to trans,trans-farnesyl diphosphate (FPP) or to cis,trans-FPP to form long-chain polyprenyl diphosphates. A few can also catalyze the condensation of IPP to trans-geranyl diphosphate to form the short-chain cis,trans- FPP. In prokaryotes, the cis-IPPS, undecaprenyl diphosphate synthase (UPP synthase), catalyzes the formation of the carrier lipid UPP in bacterial cell wall peptidoglycan biosynthesis. Similarly, in eukaryotes, the cis-IPPS, dehydrodolichyl diphosphate (dedol-PP) synthase catalyzes the formation of the polyisoprenoid glycosyl carrier lipid dolichyl monophosphate. cis-IPPS form homodimers and are mechanistically and structurally distinct from trans-IPPS, which lack the DDXXD motifs, yet require Mg2+ for activity.
Statistics
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PSSM-Id: 259850
Aligned: 670 rows
Threshold Bit Score: 161.772
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitedimer interface
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:4HE8; Staphylococcus aureus Undecaprenyl Diphosphate Synthase with bound farnesyl diphosphate and phosphate, contacts 4A
  • Structure:3UGS; Campylobacter jejuni UPP synthase binds farnesyl monophosphate; contacts at 4A.
  • Structure:2VG3; Mycobacterium tuberculosis Rv2361 (decaprenyl diphosphate synthase) binds geranyl diphosphate, Mg, and phosphate; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          ######        #   #   #  #                   #        #   #      ##  #     
1JP3_A     20 HVAIIXDGNGRWAKKQGKIRAFGHKAGAKSVRRAVSFAANNG-IEALTLYAFSSENW-NRPAQEVSALXELFVWALDSEV 97  Escherichia coli
4H8E_A     27 HIAIIMDGNGRWAKKRKMPRIKGHYEGMQTIKKITRIASDIG-VKYLTLYAFSTENW-SRPESEVNYIMNLPVNFLKTFL 104 Staphylococcus aur...
ADN50328    8 HIGVIPDGNRRWARKVGLPITEAYRIGSDKVEEFLDWSLDFG-IKIVTVYVLSTENFlRRSKLELDLLYRLLKEKLIKVR 86  Vulcanisaeta distr...
Q8ZU54     12 HIAVIPDGNRRYAKKTGIDFYHAYKKGVEKVRNFLTWALEFRdIKNVTFFALSTENL-QRSKIELEILFRIFEDELRRTL 90  Pyrobaculum aeroph...
ABW01621   11 HIGIIPDGNRRWAMAHGMDIYTAYLIGSDKVEEVLEWALDNN-VQAVTVYILSYENFmNRSEMEKRILYELLIRKLKKAR 89  Caldivirga maquili...
ADL13346    6 HIGIIPDGNRRWAESKGLSKEQGYTHGLSPGVKLLRLCKEVG-VNEVTYYGFTTDNT-ERPPKQITAFRQACVDAVEMLA 83  Acetohalobium arab...
ADG81795   12 HIGVIPDGNRRWAEKNGLPKEAGYSWGIAPGFYLYELASELG-IEEMTFYGFTHDNT-KRPTVQKRAFQKACVDAVFKLL 89  Thermincola sp. JR
EEA80936   15 HIAIIPDGNRRWATQHQMEKKDGYQHGLNPGLEVLRKAKEYG-VAEITYYGFTVDNC-KRPKVQQNAFKKACVDAVRLIQ 92  Clostridium nexile...
AEB30610   10 HIGIIPDGNRRWAQENGFKKQEGYKFGIEPGLELYHLCLELG-IEEMTFYGFTVDNT-KRPAEQTKAFQKACVDAVNKLK 87  Carnobacterium sp....
AEO36278   58 HIAVMPDGNRRYAERTGAGLEHTYSAGTRLFTQASCWSFEAG-VNRLTAFAFAPRHQ-KRNAFERDALFCQTIGTLTTSL 135 Amblyomma maculatum
Feature 1                                                    #                                
1JP3_A     98 Kslh---rhnVRLRIIGDTSRFNSrLQERIRKSEALTAgn-tgLTLNIAANYGGRWDIVQGVRQLaekvqqgnlqpdqID 173 Escherichia coli
4H8E_A    105 Peli---eknVKVETIGFTDKLPKsTIEAINNAKEKTAnn-tgLKLIFAINYGGRAELVHSIKNMfdelhqqglnsdiID 180 Staphylococcus aur...
ADN50328   87 Kderi-hrnrVRVRVIGRTWLLPEdVRREIALTEEATAny-sdHYLNLAVVYGGRQEIIDAIKKIlvdynsgklnisdLN 164 Vulcanisaeta distr...
Q8ZU54     91 Edpli-hdnkVRVRFIGDRSLLPGkVVKYIDELESVTKny-snYHLTIALGYGGRAEIVRCIKRVlsgev----rletFS 164 Pyrobaculum aeroph...
ABW01621   90 Edpri-hgnkVKVTLIGRWRNLPPyVVDEAIKTMNATEmy-nsHFLNLAVVYGGYQSIVDLVNTAitki------grlIN 161 Caldivirga maquili...
ADL13346   84 Ne-------dASLLVIGDRQADEF-PDELESYTEERQPfgnneIKVNFLVNYGWKWDLNSLSQADe------------VS 143 Acetohalobium arab...
ADG81795   90 Ek-------dAALLVVGNTESPAF-PRELLPFTKKRVNigkggMRVNFLVNYGWHWDLYHGMKAAlpr--------ksAA 153 Thermincola sp. JR
EEA80936   93 Keg------nVSLLVVGDSSSVHF-PEELLKYTQRKEIgi-adIKVNFLVNYGWEWDLSAIRKEGm------------EQ 152 Clostridium nexile...
AEB30610   88 Dr-------dADLLVVGNTNSPLF-PKELLPYTKRTKFgk-glMKINFLVNYGWNWDLNYGIKQNd-----------fDP 147 Carnobacterium sp....
AEO36278  136 QtldklkslgLRIRSVGQVDLLPSgLRAELAELEAATSddpreRTCLACIGYSSRHQIVLAALDMakgvkagtissedVT 215 Amblyomma maculatum
Feature 1                           #     # #                                    
1JP3_A    174 EEXLNQHVcxh--elaPVDLVIRTGgeHRISNFLLWQIAYAELYFTDVLWPDFDEQDFEGALNAFAN 238 Escherichia coli
4H8E_A    181 ETYINNHLmtk--dypDPELLIRTSgeQRISNFLIWQVSYSEFIFNQKLWPDFDEDELIKCIKIYQS 245 Staphylococcus aureus subsp. au...
ADN50328  165 EDTLFRYLyigdepypEPDLIIRTGgeHRISNFLLYETAYSELYILNKYWPEITKEDLKQALDDYSR 231 Vulcanisaeta distributa DSM 14429
Q8ZU54    165 EEELFQCLdtrgipnpEPDVVVRTGgeKRLSNFLLYQTAYSELIFLEKLWPEVEREDLVYIIEEYSR 231 Pyrobaculum aerophilum str. IM2
ABW01621  162 DDELIKLLptsflpnpFIDLVIRTGgeRRLSNFFPLESIYAELYFLDKYWPDFSREDFQEALRYYAS 228 Caldivirga maquilingensis IC-167
ADL13346  144 RRKLKNQLnsn--qvsQVDLVIRWGnrRRLSGFLPVQSVYADFYVVDEYWPDFEPEQFYEALEWYDK 208 Acetohalobium arabaticum DSM 5501
ADG81795  154 KHVFHQSLasa--disRIDLVIRWGgrRRLSGFLPVQTIYADFFIVDDLWPDFKPEHFYNALRWYEV 218 Thermincola sp. JR
EEA80936  153 ENDVMTQIhsk--disRIDLVIRWGnmRRLSGMLPVQSVYADFYVVENLWPDYKERDFDKAIRWYDK 217 Clostridium nexile DSM 1787
AEB30610  148 KQDSTKNIass--disRMDLIIRWGgrRRLSGFLPIQSIYSDFYVAEEMWPDYNLNQVHAALQWYQD 212 Carnobacterium sp. 17-4
AEO36278  216 AEFLDAYVtdi--ecpDVDMLVRCAg-RRLNDYMIMQSGYAYLNMNRKLWVDVSFWDWIWALLSYQL 279 Amblyomma maculatum

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