1JIF,1BYL,2RK9,3FCD,5CJ3,3BT3


Conserved Protein Domain Family
BLMA_like

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cd08349: BLMA_like 
Click on image for an interactive view with Cn3D
Bleomycin binding protein (BLMA) and similar proteins
BLMA also called Bleomycin resistance protein, confers Bm resistance by directly binding to Bm. Bm is a glycopeptide antibiotic produced naturally by actinomycetes. It is a potent anti-cancer drug, which acts as a strong DNA-cutting agent, thereby causing cell death. BLMA is produced by actinomycetes to protect themselves against their own lethal compound. BLMA has two identically-folded subdomains, with the same alpha/beta fold; these two halves have no sequence similarity. BLMAs are dimers and each dimer binds to two Bm molecules at the Bm-binding pockets formed at the dimer interface; two Bm molecules are bound per dimer. BLMA belongs to a conserved domain superfamily that is found in a variety of structurally related metalloproteins, including the bleomycin resistance protein, glyoxalase I, and type I ring-cleaving dioxygenases. As for the larger superfamily, this family contains members with or without domain swapping.
Statistics
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PSSM-Id: 319937
Aligned: 49 rows
Threshold Bit Score: 91.5185
Created: 26-Jan-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
ligand bindingdimer interface
Conserved site includes 14 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:1JIF_A; bleomycin-binding protein from bleomycin-producing Streptomyces verticillus complexed with copper(II)-bleomycin, by 4A distance.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                ##         #          # ##                             #
1JIF_A         8 VPVLTAvDVPANVSFWVDtLGFEKDFgd-----rdFAGVRrgDIRLHISRtehqi-----------------vadntSAW 65  Streptomyces ve...
3FCD_A        10 TPFLHIpDXQEALTLFCDtLGFELKYrh-----snYAYLElsGCGLRLLEeparkii-------------pdgiarvAIC 71  uncultured bact...
EEE38452       8 TPFVLCsSLERQIAFYCDrLGFTCTFqq-----dnYAFLRrdPVAIRLLEcparadg-------------rllgddqSFY 69  Rhodobacteracea...
YP_002518361  45 TPFLHVpDLEAALVFFVDlLGFEVPYrq-----pgYAYVHreTVGFRLLQadeatvt-------------prgaggfAYY 106 Caulobacter cre...
NP_923659      6 SPNLFVeDVGRALDFYETvLGFKRLMtlpeqapfaWGLVQagGVELMFQSfgsleedplp-------iegrqaggtlNLY 78  Gloeobacter vio...
NP_385440      6 VPEFAVsDWQASKEFYCNiLGFSCDYerp---eegFCYLSldGAELMIDQigtgrtfadgh-----lprsypfgrglNVQ 77  Sinorhizobium m...
NP_972378      7 IPELTVaNIEKTREFYVEiLQFKIEYerq---edkFIFLSleNNQMMFEQdngnwnvgi---------lehpygrgiNFE 74  Treponema denti...
NP_295064      7 VPELAVaDLATSLGFWVNlLGFGLKYerp---eegFAYLTlgNAHSMLDQigqsrtweta-------plqrplgrgiNFE 76  Deinococcus rad...
NP_353788      9 VPELAVsDWQKSRAFYCDlIGFHVVYerp---eegFAYLAlgDAQLMIDQigatrtfvsgn-----asleppfgrgmNLQ 80  Agrobacterium t...
P67925         5 IPALPVgDIKKSIGFYCDkLGFTLVHhe-----dgFAVLMcnEVRIHLWEasdegwrsrsndspvctgaesfiagtaSCR 79  Geobacillus ste...
Feature 1                                           #      # #   ### #   
1JIF_A        66 IEVTDPDALHEEWARAVstdyadtsgpamTPVGESPAG-REFAVRDPAGNCVHFTA 120 Streptomyces verticillus
3FCD_A        72 IDVSDIDSLHTKLSPALenlp----adqvEPLKNXPYGqREFQVRXPDGDWLNFTA 123 uncultured bacterium
EEE38452      70 IDVEGIDALYEKLRPRLqglp----egkvRPPFDQPYQqREFHVLDEDGTLVFFGE 121 Rhodobacteraceae bacterium KLH11
YP_002518361 107 IDVRDVDAVVAELTPRLaalp----pedvHGPVDQSYGqREFMIRAPDGGLLVFGQ 158 Caulobacter crescentus NA1000
NP_923659     79 IEVEDIDALYEKLRTQVsv---------vREPNTTFYGmREFLMLDPNGYLLTFAQ 125 Gloeobacter violaceus PCC 7421
NP_385440     78 IRVASADALVRALTDRGialylp-leerwYRRGDEEVGnRQFVVADPDGYLLRFYE 132 Sinorhizobium meliloti 1021
NP_972378     75 MTVSNLESLYRRILDFGikpfre-mtvshYSNGCEKIIqKEFLLQDPDGYLLRFTD 129 Treponema denticola ATCC 35405
NP_295064     77 ISVSDLDVPLARLMAADwplfla-peekwYRAGDHETGvRQFLVQDPDGYLVRLSI 131 Deinococcus radiodurans R1
NP_353788     81 LAVPSLQPILFRLERALiglvle-peekwYRRGTVEVGnRQFIVADPDGYLIRPFE 135 Agrobacterium tumefaciens str. C58
P67925        80 IEVEGIDELYQHIKPLGilh-------pnTSLKDQWWDeRDFAVIDPDNNLISFFQ 128 Geobacillus stearothermophilus

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