3L0Q,3GG4


Conserved Protein Domain Family
ASKHA_NBD_FGGY_D-RBK

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cd07782: ASKHA_NBD_FGGY_D-RBK 
nucleotide-binding domain (NBD) of D-ribulokinase FGGY and similar proteins
The subfamily includes vertebrate D-ribulokinase FGGY (also known as FGGY carbohydrate kinase domain-containing protein) and similar proteins, such as Saccharomyces cerevisiae D-ribulokinase YDR109C, Yersinia Pseudotuberculosis uncharacterized carbohydrate kinase that has been named glyerol/xylulose kinase. D-ribulokinase (EC 2.7.1.47) catalyzes ATP-dependent phosphorylation of D-ribulose at C-5 to form D-ribulose 5-phosphate. It is postulated to function in a metabolite repair mechanism by preventing toxic accumulation of free D-ribulose formed by non-specific phosphatase activities. Alternatively, D-ribulokinase may play a role in regulating D-ribulose 5-phosphate recycling in the pentose phosphate pathway.
Statistics
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PSSM-Id: 466800
Aligned: 65 rows
Threshold Bit Score: 578.72
Created: 19-Jun-2009
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 3 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic site [active site]
Evidence:
  • Comment:catalytic residues (Asp, Thr, Asp) conserved in the FGGY family
  • Comment:Two Asp residues probably form a metal cofactor binding site and the second Asp residue acts as a catalytic base.
  • Comment:It has been proposed that the first Asp coordinates and positions the MgATP, and (in concert with the Mg2+) stabilizes the ADP leaving group during the phospho transfer. The second Asp would act as a general base during catalysis, assisting the removal of a proton from the attacking hydroxyl group. The Thr could stabilize the transition state.
  • Citation:PMID 9448710

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             #  #                                                                       
3L0Q_A         6 YFIGVDVGTGSARAGVFDlqGRXVGQASREItxfkpkADFVEQSSENIWQAVCNAVRDAVNQadiNPIQVKGLGFDATCS 85  Yersinia pseudo...
ABE35273      19 YVIGVDVGTGSARAGLFDlaGHMVASAKRDItlfhasGSIVEQSSGEIWIAVCDAVKDALSQaavSPDQVAGIGFDATCS 98  Burkholderia xe...
OOK66283       7 TVIGVDVGSGSVRAGIFDlsGRLLSHATQKIatvhrsGSRVEQSSAEIWQAVCRCIREALARsgtTPQSVAGIGFDATCS 86  Pedobacter hima...
RJP33454      15 LYLAVDVGSYSLRAGLFDgrGERIASAAREIsvlnpqGGRYEQSSTEIWDKVLEACDEVLEAg--DRGRVAGLSFDATCS 92  Actinomycetia b...
XP_024363138  33 LFLGVDVGTGSARAGVFDgeGKLVGTANSPIqiw-kdGKFVEQSSTDIWLAICTAVKSVCAKyrlNDYDICGIGFSATCS 111 Physcomitrium p...
KUM24557       4 FVCAVDVGTGSARAGILDskGNLLGRAERPIamqqpkPDHAEHDSENIWSAVCTAVRAAREKagvAASDIVGISFDATCS 83  Mesorhizobium loti
PTQ49435      26 AYLGVDVGTGSARAGVFNseGKLLGLATSPIqmw-keGDCVEQSSTDIWLTVCAAVRAACKNaniSGAEICGIGFAATCS 104 liverwort
GAQ91191       3 YYVGVDVGTGSARAGVFSqeGRMVGTSSKEIaiw-keGDFAEQSSANIWDSVCSAVKSAVREagvDAASVKGLGFDATCS 81  Klebsormidium n...
CUU08506       4 YVIGVDVGTASARAGLFDlqGNRLAIAVEPIqihrpqAEYAEQSSEDIWRAVGEVVRRVVQEaqvPPEQVLGIGYDATCS 83  Armatimonadetes...
WP_110153807   5 LLVGVDVGTGSARAGIFTrdGKLLGRHENPIlmrrtdANFAEHDSEDIWTAVCRAVRTALDQagaDPADVVGIGFDATCS 84  Nostoc sp. 3335mG
Feature 1                                                                                        
3L0Q_A        86 LVVLDkegnpltvspsgrnEQNVIVWXDHRAITQAERINAt--kHPVLEFVGGVISPEXQTPKLLWLKQHXpntwsnVGH 163 Yersinia pseudo...
ABE35273      99 LVVLGaggrplpvgpseqaERDIIVWMDHRAVAQAERINAt--gHEVLNYVGGKISPEMETPKLLWLLENRpavfaaARQ 176 Burkholderia xe...
OOK66283      87 LVVLDnngeplavspdgeaENNIIVWMDHRATDQAERINAt--hHPVLDYVGGKISPEMETPKLLWLKENHrevydrAWQ 164 Pedobacter hima...
RJP33454      93 LVLLDgegrplsvsssgdpERNVIMWMDHRAAQEAASITSt--rGEPVRYLGGAVSPEHELPKLLWLKERMggtyaeMGE 170 Actinomycetia b...
XP_024363138 112 LVAIGtddspvsvsktgdaRRNVIVWMDHRAEEQAERINAs--kSPVLQFAGGSLSPEHQAPKLLWVKENLeeswaiAFR 189 Physcomitrium p...
KUM24557      84 LVVRNrqgdqlsvsvtgerRWDTIVWLDHRAIAEADECTRs--gHAVLDYIGGVMSPEMETPKLMWLKRNLpgtwneAGY 161 Mesorhizobium loti
PTQ49435     105 LVAIGaddspvsvswsgdaRRNVIVWMDHRAIDQAERINAt--kSPVLQHVGGALSPEMQPPKLLWVKENLkeswavAFR 182 liverwort
GAQ91191      82 LVAVGeldapvsvsrsgddARDIIVWLDHRAVDQAQRINKkfdgQEALRYVGGHISPENEPPKLMWVKEKLpeswarAKG 161 Klebsormidium n...
CUU08506      84 LVVLDahdqplsisptrdpQWNIIMWMDHRAVAEAEEINAg--dYEVLRYSGGALSPEMEPPKLLWLKRHMpevfhsAGK 161 Armatimonadetes...
WP_110153807  85 LVVRDadgqpvtvskdgedRWDTIVWLDHRALKEAEECTRt--gHEVLHYAGGVMSPEMEVPKLMWLKRHLpqswarAGH 162 Nostoc sp. 3335mG
Feature 1                                                                                        
3L0Q_A       164 LFDLPDFLTWRATKdETRSLCSTVCKWTYlg---------------hEDRWDPSYFKLVGladlldnnaAKIGATVKPXG 228 Yersinia pseudo...
ABE35273     177 FFDLTDFLTWRATGdLSRSTCTVTCKWTYla---------------hERRWDESYFRSVGlgvladeafARIGQTVVDPG 241 Burkholderia xe...
OOK66283     165 FFDLADFLTWRATGdVTRSICTVTCKWTWla---------------hENRWDPDYFRTVGldelagedfVRIGQHIAAPG 229 Pedobacter hima...
RJP33454     171 AFDLADFLAYMATGsRARSICTMVCKWGYlah-------------egAGGWDEGFFTAAGlkd---lyaKHLSGYAIRQV 234 Actinomycetia b...
XP_024363138 190 WMDLSDWLTFRATGdDTRSLCTTVCKWTYlghahleqmsskdsvamkASGWDDLFWQEIGlgdlvesgySTIGRNVAFPG 269 Physcomitrium p...
KUM24557     162 LFDLADFLTWKATGsLARSQCTLTAKWTYla--------------heKTGWRRDFFEIVGlgdl--fehGNLPEKASPVG 225 Mesorhizobium loti
PTQ49435     183 WMDLSDWLTYRATGdDTRSLCTTVCKWTYlghahlqhadptdtkamdACGWDDLFWQEIGlgdlvdgqyAKIGRSVALPG 262 liverwort
GAQ91191     162 WFDLADWLSYKSTGdDTRSLCTIVCKWTYrghahvga--seregpmpAAGWDKDFWRGIGlgdvveedfKRFGARVAFPG 239 Klebsormidium n...
CUU08506     162 FLDLADFLVYRSTGrNLRSECTMGCKWTYlp---------------aESRWDGRFLKAIGiesi--lerGRAGEAIRPLG 224 Armatimonadetes...
WP_110153807 163 MFDLADFLSWKATGsLARSQSTLTCKWTYlg--------------hrNPGWQHDFLSLVGlddl--lekAALPDGASPVG 226 Nostoc sp. 3335mG
Feature 1                                    #                                                   
3L0Q_A       229 aplghGLSQRAASEXGLIpGTAVSVSIIDAHAGTIGIlgasgvt---------------genanfdRRIALIGGTSTAHX 293 Yersinia pseudo...
ABE35273     242 tplgsGLTADAAAQLGLRtGTPVATGVIDAHAGGIGTvgad---------------------gdpeACLAYVFGTSSCTM 300 Burkholderia xe...
OOK66283     230 tpcgeGLTEQAAREMGLLpGTPVAVGLIDAHAGGIGTlgvg---------------------ggalNNLAYVFGTSSCTM 288 Pedobacter hima...
RJP33454     235 geavgAVCPDFAAKLGLDpACIVAAPLIDAHSGGIGSagaaldpar-----------edewrdvltRTLVIIMGTSNCHM 303 Actinomycetia b...
XP_024363138 270 hplglGLSHLAAQELGLKeGTPVATSLIDAHAGGIGTlesiprspgav-------sgasdgtdalcQRMVLVCGTSTCHM 342 Physcomitrium p...
KUM24557     226 ad-igPLTAQAAADLGLTeKCRVGAGVIDAYAGALGVlggfag-----------------detdisRHLALIAGTSSCVM 287 Mesorhizobium loti
PTQ49435     263 hplgvGLTPLSAQELGLLeGTPVGTSVIDAHAGGIGVlqsvprelvhpqaeekpdagevveedelsNRMVLVCGTSTCHM 342 liverwort
GAQ91191     240 yplggGLTKQAAEDLGLPeGTPVGAGLIDAHAGGVGTmagvpadvsss--------ddvsaasvfaRRLALVCGTSSCHM 311 Klebsormidium n...
CUU08506     225 es-agTLTPEAARHLGLTpRTQVAIGIVDAHAGTIGLlgavwydqe------------speltaveRALALIGGTSSCLM 291 Armatimonadetes...
WP_110153807 227 dd-lgCLTVGAAAELGLTtACHVGAGLIDAHAGALGVlgaftp-----------------dvdtidRHLALIAGTSSCVM 288 Nostoc sp. 3335mG
Feature 1                                                                                        
3L0Q_A       294 AXSRsahfisGIWGPYYSAilpeYWLNEGGQSATGALIDHIIQSHpcYPALLEQAKNKGe-tIYEALNYILRQXAgepen 372 Yersinia pseudo...
ABE35273     301 TTTRspvfvpGVWGPYFSAmvpdAWLNEGGQSVAGAAIERLLAMHpaAADAKRRAEHEGq-sLPAMLAALAVQAAdslse 379 Burkholderia xe...
OOK66283     289 TSAEsavfvpGVWGPYFSAmvpgLWLIEGGQSAAGAAIDRLLSFHpaAPQARQLAQESDl-pLPVYLADRLLQTFatase 367 Pedobacter hima...
RJP33454     304 ALSAepvfidGVWGPYFGAmvpgLWLNEGGQNATGSLLDHILRERsiYRELTEESGEAIf-aILNREVARLREADfw--- 379 Actinomycetia b...
XP_024363138 343 VVSHerhfipGVWGPFWSAmvpeLWLTEGGQSATGALLDHIVETHpaAPALANRAAHQKl-sIYDALNGILEDLAaeknl 421 Physcomitrium p...
KUM24557     288 AMSQdpqpfaGVWGPYYGAalpkLWLSEGGQSATGALLDHIIRWHgaGGEPDAAMHARIa-rRVAELRAVEGDALa---- 362 Mesorhizobium loti
PTQ49435     343 AVTKeklfipGVWGPYWSAmipqYWLIEGGQSATGGLLEHLVEDHaiAPLLGNRAVLQKi-sIYEVLNNILDNLAndasv 421 liverwort
GAQ91191     312 AVSDgslfvpGVWGPFWSGmipgLWLTEGGQSATGALLDHIFEGHsaYQALSERAKEAGc-kVTELLNRRLEEMAakega 390 Klebsormidium n...
CUU08506     292 ALSRearfvpGVWGPYYGAvlpgMWMNEGGQSAAGALIDYTLEHHiqYPVLRQQAEAEGi-tPYEVANRIIDRLAeglpy 370 Armatimonadetes...
WP_110153807 289 ALSAedrptpGVWGPYFGAvlpgVWLNEGGQSATGALLDHIIRWHgaGGEPTPERHLAIcrrVMELRQSEGRALA----- 363 Nostoc sp. 3335mG
Feature 1                                                                                        
3L0Q_A       373 --iafltNDIHXLPYFHGNRSPRANPnLTGIITGLKLsttPEDXALRYLATIQALALGTRHIIETXNQNGyniDTXXASG 450 Yersinia pseudo...
ABE35273     380 --aavlaDGLHVVPEFLGNRAPLADPhARAVIAGLGMeddLDSLVALYIAGICSVGYGLRQIIEAQANAGapiARVVISG 457 Burkholderia xe...
OOK66283     368 --avnlaAGLHVVPEFLGNRAPFADPhARATICGLGMendINNLMALYVAGLCGIGYGLRQIIDAQRECGvqtDNIVISG 445 Pedobacter hima...
RJP33454     380 -----fnKNLNVLPYFYGNRSPRADPdAWGMIDGLGLeddIPSYAKLYLSTVQALAFGTRHIIEEMERHGyriEYVVATG 454 Actinomycetia b...
XP_024363138 422 pfqsaltKDIHVLPDFNGNRSPLADPkSRGVVSGLSLdasPEDLALLYLATVQSIAYGTKHLVEHCNAHGlkiDTLLACG 501 Physcomitrium p...
KUM24557     363 -------ARLHVLPDFHGNRSPLADPhAVGVVSGLTLdssFDSLCKLYWRTAVGIALGVRHVLEALNENGyliDTLHVTG 435 Mesorhizobium loti
PTQ49435     422 phrsaltKELHVLPDFHGNRSPKADPrSKGMVSGMTLdssEVGVALLYLAALQSIAYGTRQIVEHLAAHGlkvDTFIACG 501 liverwort
GAQ91191     391 pflaaltRDLHVLPDWHGNRAPQADPsSRGVICGLTLesgEDALARLYLAAVQGIAYGTRQIIEKMNASAhkvDTIIACG 470 Klebsormidium n...
CUU08506     371 --palltRNLHVYPDHYGNRAPYADPhSRGVVDGLTLdksVEALALLYYATVQALAYNIRDVLRALQESGytiDTIFVTG 448 Armatimonadetes...
WP_110153807 364 -------NRLHVLPDFHGNRSPLGDPfALGVISGLTLdsdFDSLCRLYWRTCVSIALGVRHILETLNTRGyaiDTLHVTG 436 Nostoc sp. 3335mG
Feature 1                                                                                        
3L0Q_A       451 GGtKNPIFVQEHANATGCAXLLPEeseAXLLGSAXXGTVAAgvfesLPEAXAAXSriGKTVTPqt-nkIKAYYDRKYRVF 529 Yersinia pseudo...
ABE35273     458 GAgRLDLVRQLLADATGKPVLATQaeePVLLGAAMLGSVAGgqfgdVRSAMARMSqiSKTYQPdt-gkIAGLHEARYRAF 536 Burkholderia xe...
OOK66283     446 GAgQHPFVRQLLADTCGLPVLATQcsePVLLGSAILGAVAGniapsVTDAMKQFThvDKYFYPqa--nYQSLHLRRYEAY 523 Pedobacter hima...
RJP33454     455 GHiRNELLLQEHANVLERPVYLTQeteNMLLGGAVNAAAAAaryptVIAAMANMTrmGRRITPqp--eRYDLYRRKYEVF 532 Actinomycetia b...
XP_024363138 502 GLsKNKLYIQEHANIVGFPVGLPReneAVLLGAAVLGAVAAkkyssVRDAMVALNapGLVVEPsqderVKKYHNAKYEIF 581 Physcomitrium p...
KUM24557     436 GHtKNPLLMELYADATGCTVIEPLadeAVLLGTGMVAATAAglyadLNAACVAMQqgGKTRPSnp--aAGARFDRDYRIF 513 Mesorhizobium loti
PTQ49435     502 GLsKNRVYLQEHADILGCPVTLPReteCVLLGGAIIGAVAAkkyvsIDSAMIALNapGLVVQPskdikIKKYHDAKYEIF 581 liverwort
GAQ91191     471 GLaKNSLFLKQHADITECPVILPResePVLLGAAIVGAVAArqfesIADAMQAMNshGKIVLPvkaeiEKTYHAFKFSIF 550 Klebsormidium n...
CUU08506     449 GSvKNRVWLREHADITGCRIVINRepeAVLLGSAILGAVASgaypdIPSAMKAMSqpEQVIEPnp--aTARYHAVKFELF 526 Armatimonadetes...
WP_110153807 437 GHtRNPLLMELYADATLCTVVVPSspdPTLLGMAMVASRAAglfdsLDKACLAMNqgHTTRAPnt--eAQAQFDRDYRIF 514 Nostoc sp. 3335mG
Feature 1                   
3L0Q_A       530 HQXYHDHXRYQ 540 Yersinia pseudotuberculosis
ABE35273     537 LKLQSVAREIR 547 Burkholderia xenovorans LB400
OOK66283     524 KQLQQTATVIR 534 Pedobacter himalayensis
RJP33454     533 KRMYDYQREIK 543 Actinomycetia bacterium
XP_024363138 582 RSLYEQQVSYR 592 Physcomitrium patens
KUM24557     514 LEMHRQRQALD 524 Mesorhizobium loti
PTQ49435     582 KSLYEQQCAFR 592 liverwort
GAQ91191     551 EDLYVSQVKYR 561 Klebsormidium nitens
CUU08506     527 REMYQQHRARR 537 Armatimonadetes bacterium GBS
WP_110153807 515 LEMLRQRQAID 525 Nostoc sp. 3335mG

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