2W5A,2JAV,4A4X


Conserved Protein Domain Family
STKc_Nek2

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cd08217: STKc_Nek2 
Click on image for an interactive view with Cn3D
Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase 2
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The Nek2 subfamily includes Aspergillus nidulans NIMA kinase, the founding member of the Nek family, which was identified in a screen for cell cycle mutants prevented from entering mitosis. NIMA is essential for mitotic entry and progression through mitosis, and its degradation is essential for mitotic exit. NIMA is involved in nuclear membrane fission. Vertebrate Nek2 is a cell cycle-regulated STK, localized in centrosomes and kinetochores, that regulates centrosome splitting at the G2/M phase. It also interacts with other mitotic kinases such as Polo-like kinase 1 and may play a role in spindle checkpoint. An increase in the expression of the human NEK2 gene is strongly associated with the progression of non-Hodgkin lymphoma. Nek2 is one in a family of 11 different Neks (Nek1-11) that are involved in cell cycle control. It The Nek family is part of a larger superfamily that includes the catalytic domains of other STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 270857
Aligned: 39 rows
Threshold Bit Score: 364.94
Created: 3-Aug-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:2W5A_A; human Nek2 binds MgADP; defined at 4A contacts.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1               ## ##   #            # #                               #                 
2W5A_A         7 DYEVLYTIGTGSYGRCQKIRRksDGKILVWKELDYGSMteaekQMLVSEVNLLRELk-HPNIVRYYDRIIdrtnttLYIV 85  human
AAM11351      18 DYEVLAVMGNGSFGTCYKVRDksTGELFAWKGMNYDELdeakcDALVSEISVLRQLq-HPNIVQYYHHLVnreaksVYIV 96  fruit fly
XP_667586     15 DYEEIEEIGRGCFGTVHKIRRksDGRLFVWKKICYENMtqqekIQIVNEVNVLRKLn-HRNIIKYIDRIIdkqnqqIYII 93  Cryptosporidium...
CAB76949      14 EYEVIKKIGNGRFGEVFLVKHkrTQEFFCWKAISYRGLkerekSQLVIEVNVMRELk-HKNIVRYIDRFLnkanqkLYIL 92  malaria parasit...
XP_001314217   9 GYEIKDFIGKGSFGQAYIAIRksDNKTVALKQINYGNMsthekEMLVNECNILKKLr-NDNIVTYYDRIVdtsqrtLFLI 87  Trichomonas vag...
XP_001321059   4 KYEIIERLGQGSFGSVCKAIRksDQKVVAIKEISYSQMdnkkkQMLINEVNALRNIq-NEHVVGYYDRIVvrekklMYIV 82  Trichomonas vag...
EDO78975      34 QYIFLNIIGSGSFGKVHKVQSrtNGRIFACKEIDYAKMsekekKLLVHEVNTLKELs-HENIVSYIDRFVerenakMFIV 112 Giardia lamblia...
EDO82453       9 AYDFVAICGAGSFGKVHKVRSkeDGQIYACKEINYAKMsskekELLVSEVNLLRKLn-HPNIVKYIRRYQdkaatlIYIV 87  Giardia lamblia...
EGF82862       3 NYEPLEAVGSGSFGIIRKVRRkeDGKMLARKEIDYRKMsekekKQLVAEVNILRELr-HPNIVRYYERFVdrqnclIFII 81  Batrachochytriu...
GAA97424      62 IYEPLEMIGKGSFGVIRKVRRrrDGAVFVRKEINYLKMnerdlKQLSEEVNILQNMesNYHIVRYYERFVdkaeyvVFII 141 Mixia osmundae ...
Feature 1         ###   #                                                        # #             
2W5A_A        86 MEYCEGGDLASVITkgtkerqYLDEEFVLRVMTQLTLALKECHrrs----dggHTVLHRDLKPANVFLDg---------- 151 human
AAM11351      97 MECCAGGDLAQIVQrarsqrqRFEEPYIWRVLFQLCRALQVCHnki-----pnGTILHRDIKPANIFLDa---------- 161 fruit fly
XP_667586     94 MEYCDEGDLGNILKkkkklglSIDEETVISIFIQLLDALNYCHtr-------sNRVLHRDIKPQNIFMIassspelknki 166 Cryptosporidium...
CAB76949      93 MEFCDAGDLSRNIQkcykmfgKIEEHAIIDITRQLLHALAYCHnlkd--gpngERVLHRDLKPQNIFLStgirhigkis- 169 malaria parasit...
XP_001314217  88 MEYCSGGDLQKFIQns---tsPIGENQIWLSLSELALALNECHng-------kERIIHRDIKPGNIFIDs---------- 147 Trichomonas vag...
XP_001321059  83 MEYCEKGDLQQLIRktradndRIPESAIWQVLTDMCIALDSCH----------PKMIHRDIKPGNIFISg---------- 142 Trichomonas vag...
EDO78975     113 MEYCENGDLARYIKrhktdrrYIAEEKIWSVFVQLLHALNYCHslhkhddtgtHKVIHRDIKPGNVFLTr---------- 182 Giardia lamblia...
EDO82453      88 MDYCSKGDLSDYVKmhkktnqYISEDKIWSIFAQLLIALDYCHspnkpdssgvGRVIHRDLKTANVFLCe---------- 157 Giardia lamblia...
EGF82862      82 MEYCEGGDLAAVIKrckkegrFIPEEIVWNLLGQLLLALQECHasd-----khPTILHRDIKPDNVFLDk---------- 146 Batrachochytriu...
GAA97424     142 MEYCAGGDLSALIGrnrrdntLIPEDLVWSYLTQIVIALSDCHsetdsqnrpkQVILHRDLKPENVFLDk---------- 211 Mixia osmundae ...
Feature 1                                    #                                                   
2W5A_A       152 ----------------kQNVKLGDFGLARILNHDTs-fAKTFVGTPYYMSPEQMNRMs--YNEKSDIWSLGCLLYELCaL 212 human
AAM11351     162 ----------------aGNAKLGDFGLARMLRRDQs-fAASFVGTPHYMSPELVKGRk--YDRKSDVWAVGCLVYEMCaL 222 fruit fly
XP_667586    167 qegsrinkdldkpeqktMIVKLGDFGLARYLTGRNq-lATTHVGTPYYMSPEVLGKGe--YDEKSDIWSLGCCIYEILaG 243 Cryptosporidium...
CAB76949     170 --------sqannlnsrPIAKIGDFGLSKNIGIESm--AHSCVGTPYYWSPELLLHEtksYDDKSDMWALGCIIYELCsG 239 malaria parasit...
XP_001314217 148 ----------------sGHVKLGDFGLAKPIPDGS---TKTYLGTPLYMSPELVSHHs--YDDKSDIWALGCVIYEMAaK 206 Trichomonas vag...
XP_001321059 143 ----------------nGHVKLGDFGLAREIEDGA---ASTYAGTPPYMSPELISHQp--YDEKSDIWALGCVLFELAdL 201 Trichomonas vag...
EDO78975     183 ----------------dGSIKLGDFGLCRSLGELSq--AKTNVGTPLYMAIEVLQKQs--YTEKADIWSLGCVIYELCaL 242 Giardia lamblia...
EDO82453     158 ----------------dGSIKLGDFGLCRVLEQSTm--AKTNVGTPLYMAPELLENKs--YTEKVDIWSLGCIIYELCaL 217 Giardia lamblia...
EGF82862     147 ----------------lLNVKLGDFGLSRVIENPEvdfAKTYVGTPFYMSPELVDESr--YNAKSDIWALGCLIYELCaL 208 Batrachochytriu...
GAA97424     212 ----------------dNMIKLGDFGLSKAMASAAf--TNTYVGTPYYMSPELINGQv--YDAKSDIWALGCLLYEICaH 271 Mixia osmundae ...
Feature 1                                                                    
2W5A_A       213 MPPFTAfs-qKELAGKIREGKFRRIpyrYSDELNEIITRMLNLkDYHRPSvEEILENPLI 271 human
AAM11351     223 RPPFRGra-fDQLSEKIAQGEFSRIpaiYSTDLQEIIAFMLAVdHEQRPGiEVIIRHPLV 281 fruit fly
XP_667586    244 RPPFYArs-yDELRKYVKDGLVPDLpkfYSSELNSVLKLMFERdPHKRPSaEEIFNLDFI 302 Cryptosporidium hominis TU502
CAB76949     240 KTPFHKannfAQLISELKRGPELPIk-gKSKELNLLIKNLLNLsAKERPSaLQCLGYQII 298 malaria parasite P. falciparum
XP_001314217 207 SPPFRAyg-qQQLNGKIKYAEVRRIpseYSEELWKVISSMLDKdPSKRPSaSDILQVRNV 265 Trichomonas vaginalis G3
XP_001321059 202 HLPFNGs--nEVITQLIQTGPLRRIpamYSDDLFEVIQSMMMKkPQERPTvKQLLENKYV 259 Trichomonas vaginalis G3
EDO78975     243 QPPFVAsn-mDSLKAKVKQGARPAIpahYSSDLSAVIDSMLNSnFNSRPSaAELLNHRKI 301 Giardia lamblia ATCC 50803
EDO82453     218 QPPYVAts-lDSLKAKVKRGVRPFVpnhFSPDLRRIIDMMLIKePEKRPStTELIQLPVI 276 Giardia lamblia ATCC 50803
EGF82862     209 EPPFQAnt-qAALSAKIKSGKLLPLssiYSQELQAIIRIMLTTkHELRPEtTELLAHGHI 267 Batrachochytrium dendrobatidis JAM81
GAA97424     272 HPPFHQaqtqPELTKLIREGKIPSLprgYSSNLSSVIKSMLRQnPKLRPTtAQLKTLEQY 331 Mixia osmundae IAM 14324

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