2OCC,1M56,1AR1


Conserved Protein Domain Family
Cyt_c_Oxidase_I

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cd01663: Cyt_c_Oxidase_I 
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Cytochrome C oxidase subunit I. Cytochrome c oxidase (CcO), the terminal oxidase in the respiratory chains of eukaryotes and most bacteria, is a multi-chain transmembrane protein located in the inner membrane of mitochondria and the cell membrane of prokaryotes. It catalyzes the reduction of O2 and simultaneously pumps protons across the membrane. The number of subunits varies from three to five in bacteria and up to 13 in mammalian mitochondria. Only subunits I and II are essential for function, but subunit III, which is also conserved, may play a role in assembly or oxygen delivery to the active site. Subunits I, II, and III of mammalian CcO are encoded within the mitochondrial genome and the remaining 10 subunits are encoded within the nuclear genome. Subunit I contains a heme-copper binuclear center (the active site where O2 is reduced to water) formed by a high-spin heme (heme a3) and a copper ion (CuB). It also contains a low-spin heme (heme a), believed to participate in the transfer of electrons to the binuclear center. For every reduction of an O2 molecule, eight protons are taken from the inside aqueous compartment and four electrons are taken from cytochrome c on the opposite side of the membrane. The four electrons and four of the protons are used in the reduction of O2; the four remaining protons are pumped across the membrane. This charge separation of four charges contributes to the electrochemical gradient used for ATP synthesis. Two proton channels, the D-pathway and K-pathway, leading to the binuclear center have been identified in subunit I. A well-defined pathway for the transfer of pumped protons beyond the binuclear center has not been identified. Electrons are transferred from cytochrome c (the electron donor) to heme a via the CuA binuclear site in subunit II, and directly from heme a to the binuclear center.
Statistics
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PSSM-Id: 238833
Aligned: 90 rows
Threshold Bit Score: 647.619
Created: 10-Apr-2003
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:Binuclear center (heme a3/CuB) [ion binding site]
Evidence:
  • Comment:Heme a3 and CuB form the binuclear center, where O2 is reduced to water.
  • Structure:1AR1_A: H411 is the axial ligand of heme a3; CuB ligands are H276, H325, and H326
  • Comment:Electron transfer is believed to proceed from CuA in subunit II via heme a to the binuclear center.
  • Citation:PMID 9443344

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
2OCC_N     10 TNHKDIGTLYLLFGAWAGMVGTALSLLIRAELGQPgtl-----------------lgDDQIYNVVVTAHAFVMIFFMVMP 72  cow
1AR1_A     26 TNHKDIGILYLFTAGIVGLISVCFTVYMRMELQHPgvqymclegarliadasaectpNGHLWNVMITYHGVLMMFFVVIP 105 Paracoccus denitri...
BAD99495    9 TNHKDIGTLYFTLAAWAGMMGTSLSVLIRIELSQPgsf-----------------igSDQTYNALVTAHAFIMIFFMVMP 71  Leptotrombidium pa...
YP_220656  11 TNHKDIGTLYFLFGVGPGMPGSVTSLLIRMKLIQPges-----------------sgGDSLYNMFITYHALTMIFFMVMP 73  central bearded dr...
AAU20751    7 TNHKDIGTLYFLVGIWTGLVATSMSLLIREELGQPgtl-----------------lgDDQIYNCLITAHGLLMMFFVVLP 69  tube worm
BAB39492    8 TNHKDIGSLYLIFGIWSGLIGTAFSFLIRSELSQPgsi-----------------lnDSQLYNSIITAHGLIMIFFFVMP 70  Pseudostylochus in...
NP_758778  18 TNHKDIGTLYFIFGTWSGIIGTSLSVIIRMELSQGggv-----------------inNGQIYNVVVTSHAFVMIFFFVMP 80  Ciona intestinalis
BAD86512    8 TNHKDIGTLYFLFGMWAGMIGTGMSVLIRIELGKAgtv----------------wadDGQVYNVMVTAHAFVMIFFFVMP 71  Doliolum nationalis
NP_038239  12 TNHKDIGTLYFLFGVWAGFVGTGMSVFIRMELSQVgqv-----------------igDGQLYNVVVTAHAFVMIFFFVMP 74  Halocynthia roretzi
BAD06236   12 TNHKDIGTLYFIFGAWAAMMGTALSAIIRIELGSPgty-----------------inDGHLYNAIVTAHAFMMIFFAVMP 74  sea firefly
Feature 1                                                                                     
2OCC_N     73 iMIGGFGNWLVPLMIGAPDMAFPRMNNMSFWLLPPSFLLLLASSMve-----aGAGTGWTVYPPLAGNlahaGASVDLTI 147 cow
1AR1_A    106 aLFGGFGNYFMPLHIGAPDMAFPRLNNLSYWMYVCGVALGVASLLapggndqmGSGVGWVLYPPLSTTe--aGYSMDLAI 183 Paracoccus denitri...
BAD99495   72 aMIGGFGNWLIPMMIGSPDMAFPRLNNMSFWLLIPSLFFLSMSMFsg-----sGAGTGWTVYPPLSGNlahsGFSVDLTI 146 Leptotrombidium pa...
YP_220656  74 iMIGGFGNWLVPLMLGAPDMAFPRLNNMSFWLLPPSFLLLMSTAWfn-----sGVGTGWTIYPPLSGNlahaGPSMDLAI 148 central bearded dr...
AAU20751   70 iLMGGFGNWLVPLMLGAPDMAFPRINNLGFWLIPPAVILLVMSAFie-----kGAGTGWTVYPPLASNiahaGPCIDLAI 144 tube worm
BAB39492   71 vLIGGFGNWLIPLYLASPDMAFPRLNNMSFWLLPPAFFLLLGSFIve-----gGVGGGWTIYPPLSSNiahsGPSADMAI 145 Pseudostylochus in...
NP_758778  81 iMVGGFGNWLIPLMVGAPDMAFPRMNNMSFWLLPPSFFLLLKSGFsg----saGVGTGWTVYPPLSANighsNTGVDMAI 156 Ciona intestinalis
BAD86512   72 vTLGGFGNWLIPLMVGAPDMAFPRMNNLSFWLLPPAFFLLLLSSFvg-----gGAGTGWTVYPPLSGNlahaGPSVDCAI 146 Doliolum nationalis
NP_038239  75 vMIGGFGNWLLPLMLGCPDMAFPRLNNMSFWLLPPALFMLLLSSVie-----sGVGTGWTVYPPLSGNlahsGPALDCAI 149 Halocynthia roretzi
BAD06236   75 iLIGGFGNWLVPLLLGSPDMAFPRLNNLSFWLLPPSLLLLLVSSLvd-----kGVGAGWTVYPPLSGLvgqnGPSMDLAI 149 sea firefly
Feature 1                                                                                     
2OCC_N    148 FSLHLAGVSSILGAINFITTIINMKPpamsqyqtPLFVWSVMITAVLLLLSLPVLAAGITMLLTDRNLNTTFFDpaGGGD 227 cow
1AR1_A    184 FAVHVSGASSILGAINIITTFLNMRApgmtlfkvPLFAWSVFITAWLILLSLPVLAGAITMLLMDRNFGTQFFDpaGGGD 263 Paracoccus denitri...
BAD99495  147 FSLHLAGISSILGAINFITTILSATAntw--ermPLFVWSVLVTTILLLLSLPVLAGAITMLLTDRNFNTSFFDpsGGGD 224 Leptotrombidium pa...
YP_220656 149 FALHLAGASSILGAINFITTCINMSPhhmspynwPLFVWSVFLTAILLLLSLPVLAAAITMLLTDRNLNTTFFDpnGGGD 228 central bearded dr...
AAU20751  145 FALHLSGISSILASINFITTVMNMRYkglrlervPLFVWSVKLTAVLLLLSIPVLAGGLTMLLTDRNLNTSFFDpaGGGD 224 tube worm
BAB39492  146 FSLHLAGVSSILGSINFIITMVNAKInlaw-gqlPLFLWAVMVTAYMLVLSLPVLAGGLTMLLTDRNFNTTFFDpgGGGD 224 Pseudostylochus in...
NP_758778 157 FSLHLAGVSSILGSVNFLVTLFNMKNkgksmsnlSLFCWSLIVTTILLVLSLPVLAAAITMLLFDRNFNTTFFDpnGGGD 236 Ciona intestinalis
BAD86512  147 FSLHLAGVSSILGSINFLVTMMNMMSkgqtygnlSLFCWSIVVTTILLILSLPVLAAAITMLLFDRNFNTSFFDpaGGGD 226 Doliolum nationalis
NP_038239 150 FSLHLAGVSSILGSLNFMTTMFNMGLkggglfmmPLFCWSVLITTLLLLLSLPVLAAAITMLLFGRNLNTSFFDpaGGGD 229 Halocynthia roretzi
BAD06236  150 FSLHLAGASSLAGAINFLVTIFQARSpgmtleriPLYPWAVAVTALLLVLALPVLAGAITMLLTDRNFNTSFFSpdGGGD 229 sea firefly
Feature 1                 #                                                 ##                
2OCC_N    228 PILYQHLFWFFGHPEVYILILPGFGMISHIVTYYsgkKEPFGYMGMVWAMMSIGFLGFIVWAHHMFTVGMDVDTRAYFTS 307 cow
1AR1_A    264 PVLYQHILWFFGHPEVYIIILPGFGIISHVISTFa-kKPIFGYLPMVLAMAAIGILGFVVWAHHMYTAGMSLTQQAYFML 342 Paracoccus denitri...
BAD99495  225 PILFQHLFWFFGHPEVYILILPGFGLISHILSISsgkKEPVGSMAMVHAILSIGILGFIVWGHHMFTVGMDVDSRAYFSA 304 Leptotrombidium pa...
YP_220656 229 PILFQHLFWFFGHPEVYILILPGFGMISHIVTHHsskKEPFGYMSMVWAMLAITVLGFIVWAHHMFTVGLDVDTRAYFSA 308 central bearded dr...
AAU20751  225 PVLYQHLFWFFGHPEVYILVLPGFGLISHLVAHHsakLEPFGSLGMIYAMMGIGLIGFLVWAHHMFTIGMDVDTRAYFTA 304 tube worm
BAB39492  225 PILFQHIFWFFGHPEVYILILPGFGMISQIVMFYsgkDSAFGYMGMVYAILGIGFLGFIVWAHHMYTVGLDIDTRAYFTG 304 Pseudostylochus in...
NP_758778 237 PILYQHLFWFFGHPEVYILILPGFGMISHVIVFYsgkDNIFGYYGMVWAMGGIGFLGFLVWAHHMFSVGMDVDSRAYFTS 316 Ciona intestinalis
BAD86512  227 PILYQHLFWFFGHPEVYILILPGFGIVSHTVAYYsgkKEVFGYYGMVWAMLGIGFLGFLVWAHHMYTVGMDTDSRAYFTA 306 Doliolum nationalis
NP_038239 230 PVLYQHLFWLFGHPEAYILILPGFGMISHIIVFYtnkEVVFGFYGMVWAIIGIGFLGFLVWAHHMFTVGMDVDSRAYFTA 309 Halocynthia roretzi
BAD06236  230 MILFQHLFWFFGHPEVYILILPGFGLVSHIVIHHsskGLAFGTLGMIYAMCAIGLLGFIVWGHHMFTVGLDVDTRAYFTS 309 sea firefly
Feature 1                                                                            #        
2OCC_N    308 ATMIIAIPTGVKVFSWLATLHGGnik---wsPAMMWALGFIFLFTVGGLTGIVLANSSLDIVLHDTYYVVAHFHYVLSMG 384 cow
1AR1_A    343 ATMTIAVPTGIKVFSWIATMWGGsie---fkTPMLWAFGFLFLFTVGGVTGVVLSQAPLDRVYHDTYYVVAHFHYVMSLG 419 Paracoccus denitri...
BAD99495  305 ATMVIAIPTGVKVFNWIASLSGAyvm---ktPALLWAVGFVFLFTIGGLTGIVLANSSLDVVLHDTYYVVAHFHYVLSMG 381 Leptotrombidium pa...
YP_220656 309 ATMTIAVPTGIKVFSWAATIFGGkir---wtVPMFWAAGFIYLFTLGGLTGIMLSNSSLDTMLHDTYYVVAHFHYVLSMG 385 central bearded dr...
AAU20751  305 ATMIIAVPTGIKVFSWLATIHGSkik---ydTPMLWVLGFIFLFTVGGLTGIVVGNCSLDIMMHDTYYVVAHFHYVLSLG 381 tube worm
BAB39492  305 ATMIIAVPTGIKIFSWLATFYGRplakdednVGPLWATGFIFLFTLGGLTGVVLASASLDISLHDTYYVVAHFHYVLSMG 384 Pseudostylochus in...
NP_758778 317 ATMIIAVPTGIKVFSWVSTLLGAkih---wgLPLLWAYGFLFLFTIGGLTGIVLANCSLDLVLHDTYYVVAHFHYVLSMG 393 Ciona intestinalis
BAD86512  307 ATMVIGVPTGIKVFSWMGTMAGGrld---wgISFYWTAGFLFLFTIGGLTGIVLSNSSMDLVLHDTYYVVAHFHYVLSMG 383 Doliolum nationalis
NP_038239 310 ATMVIAVPTGIKVFSWVSTLVGAkiv---whIAMYWVCGFLFLFTLGGLTGIILSNCSLDLVLHDTYYVVAHFHYVLSMG 386 Halocynthia roretzi
BAD06236  310 ATMIIAVPTGVKIFSWLSTLSGAnfn--nitPSLSWACGFIVLFTFGGFTGIVLSNSSIDLALHDTYYVVAHFHYVLSMG 387 sea firefly
Feature 1                                                                                     
2OCC_N    385 AVFaIMGGFVHWFPLFSGYTLnDTWAKIHFAIMFVGVNMTFFPQHFLGLSGMPRRYSDYPDa-YTMWNTISSMGSFISLT 463 cow
1AR1_A    420 AVFgIFAGVYYWIGKMSGRQYpEWAGQLHFWMMFIGSNLIFFPQHFLGRQGMPRRYIDYPVe-FAYWNNISSIGAYISFA 498 Paracoccus denitri...
BAD99495  382 AVFaIFGGIALWVPILFSIHLsQKQMKAHFFSTFLGVNLTFFPQHFLGLNGMPRRYSDFPDg-LTFWNSISSFGALLSAL 460 Leptotrombidium pa...
YP_220656 386 AVFaIMEGLVHFHAMLTGYSLnSTLAKAQFWAMFTGVNITFFPQHMLGLAGMPRRYSDFPDa-YAIWNTISSMGSIMSLV 464 central bearded dr...
AAU20751  382 AVFaIFGGLTHYFPLFTGVTLhSRWSKSHFFMMFTGVNLTFFPQHFLGLSGMPRRYSDYPDv-YTTWNVLSSIGAFISFT 460 tube worm
BAB39492  385 AVFsIFAGLVHWWVIFTGVALnSKMASAQFWIMFTGVNLTFFPQHFLGLSGMPRRYSDYPDg-FAYWNSISSFGSLVSVI 463 Pseudostylochus in...
NP_758778 394 AVFaIFSGFFHWSPLFTGLSFnLILGQIHFFMMFVGVNLTFFPQHFLGLSGMPRRYNDYPDf-YLTWNVVSSIGSLLSGV 472 Ciona intestinalis
BAD86512  384 GVFaVFAGFTHWYPVFTGLSFnKILGEAHFYTTFIGVNLTSFPQHFLGLTGMPRRYNDFPDf-FLAWNTVSSVGSMVSVF 462 Doliolum nationalis
NP_038239 387 AVFaIFAGFAHWYSLFTGLQMsHYLGLVQFWVMFIGVNLTFFPQHFLGLAGMPPRRYNDFSdfYLFWKVASSIGSLMSSL 466 Halocynthia roretzi
BAD06236  388 AVFaILGGIVHWFPLFTGYTLnNKYANWQFILMFLGVNLTFFPQHFLGLSGMPRRYTDYPDi-YATWHFISSVGSLISTV 466 sea firefly
Feature 1                                               
2OCC_N    464 AVMLMVFIIWEAFaskrevlt---------vdltttnlEWLN 496 cow
1AR1_A    499 SFLFFIGIVFYTLfagkrvnvpn------ywnehadtlEWTL 534 Paracoccus denitrificans
BAD99495  461 SAFFFLWILWEGSvvsrktis---------hnisyssgEWAQ 493 Leptotrombidium pallidum
YP_220656 465 SALTMIVILWEAFskkrsttp---------attdaklqEWTQ 497 central bearded dragon
AAU20751  461 SLMFFIFLMWEALasqrgvla---------sphmptalEWQE 493 tube worm
BAB39492  464 GVVFFIVIVWESIlservvly---------vispnsqlEWIG 496 Pseudostylochus intermedius
NP_758778 473 AVVVFFLMLWESFyskrflftveskslefklgfppalhTWMT 514 Ciona intestinalis
BAD86512  463 GVVLFLVMLWESMtvmrfastyeld-alefaevppqahTWME 503 Doliolum nationalis
NP_038239 467 GALLFGCMIWESMisnrkvya---------aeetgsylEWSC 499 Halocynthia roretzi
BAD06236  467 ALILFVVLLWEALiacrpvmf---------tthpstniEWSH 499 sea firefly

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