Conserved Protein Domain Family
ASKHA_NBD_HSP70_HSPA12B
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cd11736: ASKHA_NBD_HSP70_HSPA12B 
nucleotide-binding domain (NBD) of heat shock 70 kDa protein 12B (HSPA12B) and similar proteins
HSPA12B, predominantly expressed in endothelial cells, is required for angiogenesis, and may interact with known angiogenesis mediators. It may be important for host defense in microglia-mediated immune response. HSPA12B belongs to the heat shock protein 70 (HSP70) family of chaperones that assist in protein folding and assembly, and can direct incompetent "client" proteins towards degradation. Typically, HSP70s have a nucleotide-binding domain (NBD) and a substrate-binding domain (SBD). The nucleotide sits in a deep cleft formed between the two lobes of the NBD. The two subdomains of each lobe change conformation between ATP-bound, ADP-bound, and nucleotide-free states. ATP binding opens up the substrate-binding site; substrate-binding increases the rate of ATP hydrolysis. HSP70 chaperone activity is regulated by various co-chaperones: J-domain proteins and nucleotide exchange factors (NEFs). No co-chaperones have yet been identified for HSPA12B.
Links
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Statistics
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PSSM-Id: 466842
Aligned: 5 rows
Threshold Bit Score: 736.005
Created: 6-Apr-2012
Updated: 27-Apr-2023
Structure
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Aligned Rows:
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Feature 1:putative nucleotide binding site [chemical binding site]
Evidence:
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1             # ####                                                                     
Q96MM6        61 VVVAIDFGTTSSGYAFSFAsdpeaihmmrkweggdpgvAHQKTPTCLLLTPEgaFHSFGYTARDYYHDLdpeeardwlyf 140 human
NP_001036151  61 VVVAIDFGTTSSGYAFSFIedpetihmmrrweggdpgvANQKSPTCLLLTPDlrFHSFGFAARDSYHDLdpeearhwlyf 140 zebrafish
XP_018082839  62 VVVAIDFGTTSSGYAFSFSkdpeaihmmrkweggdpgvAHQKTPTSLLLTPDkaFHSFGYTARDYYHDLdpeeardwlyf 141 African clawed ...
XP_006010284  61 VVVAIDFGTTSSGYAFSFTrdpeaihmmrrweggdpgvANQKTPTSLLLTPDgkFHSFGFTARDYYHDLdpeearewlyf 140 coelacanth
PKU46810     197 VVVAIDFGTTSSGYAFSFSsdpeaihmmrkweggdpgvANQKTPTSLLLTPEgvFHSFGYTARDYYHDLdpeeardwlyf 276 Limosa lapponic...

Feature 1                                                                                        
Q96MM6       141 ekfkmkihsatdltlktqLEAVNGKTMPALEVFAHALRFFREHALQELreqspslpekdtVRWVLTVPAIWkQPAKQFMR 220 human
NP_001036151 141 dkfkmkihstsdltmeteLESVSGRRVQAIEVFAHALRFFREHALKEVkdqsssvlegneVRWVITVPAVWrQPAKQFMR 220 zebrafish
XP_018082839 142 ekfkmkihstsdltmkteLEAVNGKKVQAMVVFAHALRFFKEHAVQELkdqcpalnekeaIRWVLTVPAIWkQPAKQFMR 221 African clawed ...
XP_006010284 141 ekfkmkihstsdltmeteLEAVNGKKLKALEVFAHALRFFKEHALQELkdqwp--isldaVRWVLTVPAIWkQPAKQFMR 218 coelacanth
PKU46810     277 ekfkmkihstsdltmqteLEAVNGKKMQALEVFAHALRFFKQHAVQELkdqcpslperdaIRWVITVPAIWkQPAKQFMR 356 Limosa lapponic...

Feature 1                             #                                                          
Q96MM6       221 EAAYLAGlvsrenaeQLLIALEPEAASVYCRKLrlhqlldlsgrapgggrlgerrsidssfrq-areqlrrsrhsrtflv 299 human
NP_001036151 221 EAAYLAGlvppdspeQLLIALEPEAASIYCRKLrlhqvtdlsqrpvtngfdidgsrpfdssfrqareqlrrarhsrtflv 300 zebrafish
XP_018082839 222 EAAYLAGlvspenpeQLLIALEPEAASIYCRKLrlhqlidlsskaiingypsdrsidssfrqa--reqlrrsrhsrtflv 299 African clawed ...
XP_006010284 219 EAAYLAGlvspenpeQLLIALEPEAASIYCRKLrlhqlidlsfkclangfgsespldssfrqa--reqlrrsrhsrtflv 296 coelacanth
PKU46810     357 EAAYKAGlvspenpeQLLIALEPEAASIYCRKLrlhqlidlsckppanglapdhsidssfrqa--reqlrrsrhsrtflv 434 Limosa lapponic...

Feature 1                            # ####                           ##                         
Q96MM6       300 esgvgelwaemqagDRYVVADCGGGTVDLTVHQleqphgtlkelykasGGPYGAVGVDLAFEQLLCRIFGedfiatfkrq 379 human
NP_001036151 301 esgtgelwsemqtgDRYIVADCGGGTVDLTVHQieqpqgtlkelykasGGPYGAVGVDLAFETMLCQIFGtdfidsfkak 380 zebrafish
XP_018082839 300 etgmgelwsemeagDRYIVADCGGGTVDLTVHQieqpkgtlkelykasGGPYGAIGVDLAFEKMLCQIFGedfiqtfkvk 379 African clawed ...
XP_006010284 297 esgmgelwsemqagDRYVVADCGGGTVDLTVHQieqpqgtlkelykasGGPYGAVGVDLAFEKLLCEIFSsdfietfkak 376 coelacanth
PKU46810     435 esgvgelwsemqagDRYIVADCGGGTVDLTVHQiekpqgtlkelykasGGPYGAVGVDLAFEKLLCHIFGedfiatfkak 514 Limosa lapponic...

Feature 1                    #  ##  #                                                            
Q96MM6       380 rPAAWVDLTIAFEARKRTAGphragalnislpfsfidfyrkqrghnvetalrrssvnfvkwssqgmLRMSCEAMNELFQP 459 human
NP_001036151 381 rPAAWVDLTIAFEARKRTAApgrantlnislpfsfidfykqhrgqsvetalrksnmnfikwssqgmLRLSTEATNELFQP 460 zebrafish
XP_018082839 380 rPAAWVDLMIAFEARKRTAApqrsnhlnislpfsfidfyrknkgqnvetalrkssvnfvkwssqgmLRMSSEAMNELFQP 459 African clawed ...
XP_006010284 377 rPAAWVDLTIAFEARKRTAApnranplnislpfsfidfykkykgqtvetalkkssvnfvkwscqgmLRMTSEAMTELFQP 456 coelacanth
PKU46810     515 rPAAWVDLTIAFEARKRAAApsrasplnislpfsfidfyrkhrgqnvetalkksnvnfvkwssqgmLRMSSEAMSELFQP 594 Limosa lapponic...

Feature 1                                   ### ##                        #           
Q96MM6       460 TVSGIIQHIeallarpevqGVKLLFLVGGFAESAVLQHAVQAALGarglRVVVPHDVGLTILKGAVLFG 528 human
NP_001036151 461 TINNIIKHIenvmqkeevkGVRFLFLVGGFAESPMLQRAIQNTLGrn-cRIIIPHDVGLTILKGAVLFG 528 zebrafish
XP_018082839 460 TIRQIVQHIddlmkkpevkGIKYLFLVGGFAESPMLQNAVQSAFSni-cRVIIPQDVGLTILKGAVLFG 527 African clawed frog
XP_006010284 457 TIKQIIQHIddlikkpevkRITFLFLVGGFAESPVLQQAVQSAFGni-cRVIIPQDVGLTILKGAVLFG 524 coelacanth
PKU46810     595 TISQIIKHIddllkkpevqGIKFLFLVGGFAESAMLQRAVQAAFGht-cRVIIPQDVGLTILKGAVLFG 662 Limosa lapponica baueri

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