1DDT,1PAX,1TOX


Conserved Protein Domain Family
ADP_ribosyl
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cd01341: ADP_ribosyl 
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ADP_ribosylating enzymes catalyze the transfer of ADP_ribose from NAD+ to substrates. Bacterial toxins are cytoplasmic and catalyze the transfer of a single ADP_ribose unit to eukaryotic elongation factor 2, halting protein synthesis and killing the cell. Poly(ADP-ribose) polymerases (PARPS 1-3, VPARP, tankyrase) catalyze the addition of up to 100 ADP_ribose units from NAD+. PARPs 1 and 2 are localized in the nucleaus, bind DNA, and are activated by DNA damage. VPARP is part of the vault ribonucleoprotein complex. Tankyrases regulates telomere length in part through poy(ADP_ribosylation) of telomere repeat binding factor 1 (TRF1). Poly(ADP-ribose) polymerase catalyses the covalent attachment of ADP-ribose units from NAD+ to itself and to a limited number of other DNA binding proteins, which decreases their affinity for DNA. Poly(ADP-ribose) polymerase is a regulatory component induced by DNA damage. The carboxyl-terminal region is the most highly conserved region of the protein. Experiments have shown that a carboxyl 40 kDa fragment is still catalytically active.
Links
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Statistics
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PSSM-Id: 238651
Aligned: 17 rows
Threshold Bit Score: 76.0591
Created: 12-Dec-2003
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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nad+ binding
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:nad+ binding pocket [chemical binding site]
Evidence:
  • Structure:DTT:NAD+ contacts at 4A; 1TOX_A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1        #####  #   #                  ### #                                         ##
1DDT        18 SSYHGTKPGYVDSIQK----------------GIQKPksgtqg---------------------------nydddWKGFY 54   Corynebacterium ...
1PAX       206 LLWHGSRTTNFAGILSq---------------GLRIAppeapv---------------------------tgymfGKGIY 243  chicken
1TOX_A      18 SSYHGTKPGYVDSIQK----------------GIQKPksgtqg---------------------------nydddWKGFY 54   Corynebacterium ...
XP_391017  640 FAWHGSFLGNWHSIIRt---------------GLNFSvitngraygngvyl-----------akdfqtsrsyssrGGGSS 693  Gibberella zeae ...
NP_056323  529 HLFHGTSQDVVDGICKh---------------NFDPRvcgkh-----------------------------atmfGQGSY 564  human
BAB15044   341 FAFHGSHIENWHSILRn---------------GLVVAsntrlq--------------------------lhgamyGSGIY 379  human
EAA66581   166 VGYLGDPEGSIILSASrrisklgisgeameawGVRPSqylvllirypygyrrlidvvqrpggcgmiklyagvcadYKPSL 245  Aspergillus nidu...
O95271    1181 MLFHGSPFINAIIHKGf---------------DERHAyig--------------------------------gmfGAGIY 1213 human
AAO51129   456 FLFHGLNVSSVAGIAKf---------------GFDPRfcsl------------------------------dgmfGAGLY 490  Dictyostelium di...
CAF95416   546 FAFHGSHIENWHSVLRn---------------GLVNAsytklq--------------------------lhgaayGKGIY 584  Tetraodon nigrov...

Feature 1      #                                #                                              
1DDT        55 ST-----------------------DNKYDAAGYSvdnenp----------------------lsgkaGGVVKVTYpglt 89   Corynebacterium ...
1PAX       244 FA-----------------------DMVSKSANYChtsq-------------------------adpiGLILLGEValgn 275  chicken
1TOX_A      55 ST-----------------------DNKYDAAGYSvdnenp----------------------lsgkaGGVVKVTYpglt 89   Corynebacterium ...
XP_391017  694 WPns--------------------aLRITEAISICeivndtarfvstd-------phfvvdqidwiqcRYLFIKVDiens 746  Gibberella zeae ...
NP_056323  565 FA-----------------------KKASYSHNFSkkssk------------------------gvhfMFLAKVLTgryt 597  human
BAB15044   380 LS-----------------------PMSSISFGYS---------------------------------GMNKKQKVsakd 403  human
EAA66581   246 SSalhvfaneaapgppdlgqetnkdSMEPVLHSIFigkslq----------------------allnsRFIDIVKYrlek 303  Aspergillus nidu...
O95271    1214 FA-----------------------ENSSKSNQYVygigggtgcpthkdrscyichrqmlfcrvtlgkSFLQFSTMkmah 1270 human
AAO51129   491 FA-----------------------ENSSKSNQYChggactcsgfq-----------atsckckpsdeVCLLVCRVvmgd 536  Dictyostelium di...
CAF95416   585 LS-----------------------PISSISFGYSgmgkgqhrm----------------ptkdelvqRYNRMNTIpqsr 625  Tetraodon nigrov...

Feature 1                                                                                      
1DDT        90 kvlalkvdnaetikkelgls--lteplmeqvgteefikrfgdgasrVVLSlpfa-------------------------- 141  Corynebacterium ...
1PAX       276 myelkna-----------------------------shitklpkgkHSVKglgktapdptatttldgv------------ 314  chicken
1TOX_A      90 kvlalkvdnaetikkelgls--lteplmeqvgteefikrfgdgasrVVLSlpfa-------------------------- 141  Corynebacterium ...
XP_391017  747 qasshdkngsasyieqdpshqltsnskrigipqsaipisrrrvlgpRQILgssndfpiildetggepdfesndelddlla 826  Gibberella zeae ...
NP_056323  598 mgshgmrr---------------------------pppvnpgsvtsDLYDscvd-------------------------- 624  human
BAB15044   404 epassskssntsqsqk-----------kgqqsqflqsrnlkcialcEVITssd--------------------------- 445  human
EAA66581   304 kf---------------------------------------swtgaELYMndgq-------------------------- 318  Aspergillus nidu...
O95271    1271 a-----------------------------------------ppghHSVIgrps-------------------------- 1283 human
AAO51129   537 clvenvfrgngpgq--------------fwngrrtepkkpnniniyNSVIgeskpny----------------------- 579  Dictyostelium di...
CAF95416   626 pvqsrfl----------------------------qsrnlncialcEVITskd--------------------------- 650  Tetraodon nigrov...

Feature 1                                                                                      
1DDT           --------------------------------------------------------------------------------      Corynebacterium ...
1PAX           --------------------------------------------------------------------------------      chicken
1TOX_A         --------------------------------------------------------------------------------      Corynebacterium ...
XP_391017  827 sddegeskdetaavtrkrrrssidsglgdarqpdnttpfqpgqldlgslpklveptwaasspaalralngqikdlqqiqs 906  Gibberella zeae ...
NP_056323      --------------------------------------------------------------------------------      human
BAB15044       --------------------------------------------------------------------------------      human
EAA66581       --------------------------------------------------------------------------------      Aspergillus nidu...
O95271         --------------------------------------------------------------------------------      human
AAO51129       --------------------------------------------------------------------------------      Dictyostelium di...
CAF95416       --------------------------------------------------------------------------------      Tetraodon nigrov...

Feature 1                                  #                                      
1DDT       142 ----------------------egsSSVEYINNweq-----------------------akaLSVEL 163  Corynebacterium diphtheriae
1PAX       315 --------evplgngistgindtclLYNEYIVYdv-------------------------aqVNLKY 348  chicken
1TOX_A     142 ----------------------egsSSVEYINNweq-----------------------akaLSVEL 163  Corynebacterium diphtheriae
XP_391017  907 stniamlgwyidfekldnlfhwiveLHSFDMNLal-------------------------aqDMIRY 948  Gibberella zeae PH-1
NP_056323  625 ----------------------nffEPQIFVIFnd-------------------------dqSYPYF 644  human
BAB15044   446 -----------------------lhKHGEIWVVpnt------------------------dhVCTRF 465  human
EAA66581   319 ---------------------grllVSDESTCQqkyfepdwrgsppsflkhdhlantvdpsdMSLLL 364  Aspergillus nidulans FGSC A4
O95271    1284 ---------------------vnglAYAEYVIYrg-------------------------eqAYPEY 1304 human
AAO51129   580 -------------------gpkaalQLREYIVYes-------------------------sqVFVEY 602  Dictyostelium discoideum
CAF95416   651 -----------------------lqKHGNIWVCpvs------------------------dhVCTRF 670  Tetraodon nigroviridis

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