1B8O,3F8W,1G2O,1YQQ,4NS1,1VMK,5IFK,4LNA,4UC0,2P4S,2A0W,5CXQ,3LA8,4M1E


Conserved Protein Domain Family
PNP-EcPNPII_like

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cd09009: PNP-EcPNPII_like 
Click on image for an interactive view with Cn3D
purine nucleoside phosphorylases similar to human PNP and Escherichia coli PNP-II (XapA)
Human PNP catalyzes the reversible phosphorolysis of the purine nucleosides and deoxynucleosides inosine, guanosine, deoxyinosine, and deoxyguanosine. Patients with PNP deficiency typically present with severe immunodeficiency, neurological dysfunction, and autoimmunity. Escherichia coli PNPII, product of the xapA/pndA gene, catalyzes the phosphorolysis of xanthosine, inosine and guanosine with equal efficiency and has been referred to as xanthosine phosphorylase and inosine-guanosine phosphorylase. E. coli PNPII is also capable of converting nicotinamide to nicotinamide riboside, and may be involved in the NAD+ salvage pathway. It is one of two purine nucleoside phosphorylases found in E. coli, which also contains PNPI, which displays a different substrate specificity and belongs to a different subgroup of the nucleoside phosphorylase-I (NP-I) family than PNPII. NP-I family members accept a range of purine nucleosides as well as the pyrimidine nucleoside uridine. The NP-1 family includes phosphorolytic nucleosidases, such as purine nucleoside phosphorylase (PNPs, EC. 2.4.2.1), uridine phosphorylase (UP, EC 2.4.2.3), and 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP, EC 2.4.2.28), and hydrolytic nucleosidases, such as AMP nucleosidase (AMN, EC 3.2.2.4), and 5'-methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase (MTAN, EC 3.2.2.16). The NP-I family is distinct from nucleoside phosphorylase-II, which belongs to a different structural family.
Statistics
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PSSM-Id: 350160
Aligned: 725 rows
Threshold Bit Score: 171.809
Created: 23-Jul-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3F8W: Schistosoma mansoni PNP homotrimer bound with purine nucleoside adenosine and sulphate ion, in each of three active sites; contacts at 4A
  • Structure:1G2O: Mycobacterium tuberculosis PNP homotrimer bound with immucillin-H and phosphate in each of the three active sites; contacts at 4A
  • Comment:the neighboring subunit contributes residue(s) to the protein-ligand interaction

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                             #   #                                                  #
1B8O_A      8 EDYQDTAKWLLSHteqrPQVAVICG---SGLGGLVNKltqaQTFDYSEIPNFPEstvpgHAGRLVFGilnGRACVMMQGR 84  cow
3F8W_A     10 ENVKKVAHHIQKLtsivPEIGIICG---SGLGKLADGvkdkITIPYTKIPNFPQtsvvgHSGNLIFGtlsGRKVVVMQGR 86  Schistosoma mansoni
ERL87845  367 GFIEESARFIRSRvpvaPKLLIICG---SGLGGLADTlekpVTLSYDEIPNFPQstvpgHAGELIFGtlaNVPVACMKGR 443 mountain pine beetle
KFD51774    4 AMIKKAVTFLKDDmrvtPSVGIVCG---TGRDDLSDHmrlcRSLKYNEIPGFPCprgygHPGSVIHGylgRKYVVGIETR 80  pig whipworm
KFD52565   29 DSMVAVSKFLRNQmteaPLVGLVCG---SGLGCLGEKikptVAVKYSDIPGFPVtstegHAGILLYGtlgGKRVVCMQGR 105 pig whipworm
KFD54845    4 FDVERASAYVKERvpekPAIAILSS---TGHEELADQmdkcTTIRNSDIPGLPCprtlgRQGSLLFGylgKKLTVIMPIR 80  pig whipworm
KRZ14634   19 EQCRVAANYLKLKikqnIQIAILCSenfAEIGTFLSNs---ETISCEGIPGFPVnrqncHQNNFVFGyvgKAYIGCMFAH 95  Trichinella zimbab...
KRZ68593   51 DSLLETSNFIKRKltvvPEVGLICG---TGLGELADSlsnvITLNYTDIPGFPVpsvegHEGQFVCGklgGKWIFCMRGR 127 Trichinella papuae
AEE50369    5 DQIQEALAFLRSKtdfePDFGIILG---TGLSDLAQEidrvAVFPYKEIPYFPVstvesHKGELVFGylaGRKVVAMAGR 81  Haliscomenobacter ...
OAV43561    5 DQIQECLAFIQQRcdlkPRLGIILG---TGLGSLGEEieveREFVYGLLPYFCAstvvsHRGKLILGtlhGVPVVAMSGR 81  Lewinella sp. 4G2
Feature 1      # #                         #####                                              
1B8O_A     85 FHMYEGYPFWKVTFPVRVFRLLGVETLVVTNAAGGLNPnfEVGDIMLIRDHINLPgfsgENPLRgpneerfgvrFPAMSD 164 cow
3F8W_A     87 FHMYEGYSNDTVALPIRVMKLLGVKILMVSNAAGGLNRslKLGDFVILKDHIYLPglglNNILVgpnqeafgtrFPALSN 166 Schistosoma mansoni
ERL87845  444 FHYYEGYSLQKVTTPIRIMQLLGIKGLIVTNAAGSVNKayQVGDIMLIKDHLNFFafggRHPLRgpndpqfgarFFPMNR 523 mountain pine beetle
KFD51774   81 LRSFDGYKLSFCVTPIIIMQKLGIRTLFLTTMAGKLSDqyEIGDIMLVKDHVYIPgi-fGNGIGmee----khnAPVSDK 155 pig whipworm
KFD52565  106 FHSYEGYDPAECASPIRIMHLLGARTVILTAAAGALNPdyKIGDMMVITDHFCLPalagISPLSgpnderfgprFPVMQG 185 pig whipworm
KFD54845   81 LHSYEGFTPQQCTFQVRLLHALGVPKIFLSSVAGLLNTkySIGDFMLVTDHLHMPsllgRSPLIgttnqevgprRPVMRG 160 pig whipworm
KRZ14634   96 LSTYDGYTVSETVAPVRILHLLGADLILFISNAHSLNQkfKAGDFMIIKDHLNFAelmgLSALAgryenlygiqFPNLRM 175 Trichinella zimbab...
KRZ68593  128 CHTYEGYQPIECAMPVRVMFLLGIKKVILTAAAGALNSnyKVGDVMLINDHFCLAslagISPLMgpndarfgprFPDMKG 207 Trichinella papuae
AEE50369   82 FHYYEGYTMQQITFPVRVMKFLGIQRLFISNAAGSVNQhiYAGDLIFLKDHINLQa---DNPLRghnderlgprFPDMLH 158 Haliscomenobacter ...
OAV43561   82 LHYYEGFSMQQLTFPVRVMAALGIEKLVISNASGSVNPdiEAGDVIFLRDHINMQp---ENPLRgendsrlgprFPDMLH 158 Lewinella sp. 4G2
Feature 1                                   #    ##    #          ####                     ###
1B8O_A    165 AYDRDMRQKAHSTWkqmgeqreLQEGTYVMLGGPNFETVAECRLLRNLGaDAVGMSTVPEVIVARHCGLRVFGFSLITNK 244 cow
3F8W_A    167 AYDRDLRKLAVQVAeengfgnlVHQGVYVMNGGPCYETPAECTMLLNMGcDVVGMSTIPEVVIARHCGIQVFAVSLVTNI 246 Schistosoma mansoni
ERL87845  524 AYDRALLQVGKRAAaevglanhCHEGVYGIYSGPNYETVAEIKYLQLVGvDAIGMSTVPEVLVAKHCGLRVFGFSFITNK 603 mountain pine beetle
KFD51774  156 LYKERLRDLFQIAArqveprppIHSGVYAMINGPACATETELNFLRSLKaDAVGMGICHEAVMAYNCGMEVIAAAVLGDE 235 pig whipworm
KFD52565  186 TYTRDLISIFKEAAteakfsdrVREGVYAMIGGPAFATDTESKFLRSVGaDAVGMSVVWESTVAHHSGMKVLAIAAITDV 265 pig whipworm
KFD54845  161 MYSEELRNLFKKVVgrnetkvrVHEGRYAFIGGPACPTPAELKFLSSAGaDAVGMDAGDEAMVARQCGMEVIAVATMGDP 240 pig whipworm
KRZ14634  176 AYDSEYRQILKSIAekanlekrVHEGVYTVLSNPAYPTKTEMDFIKTLGsDAIGMNLCHEVVAARQYNMRVVAIAVIRPG 255 Trichinella zimbab...
KRZ68593  208 AYSTKLRSLFRVAGkesgfsvrLHDGVYGMIGGPAFATPIENKFIRMAGsDVVGMSIVWESTIAHHCGMEVLAVAVITSI 287 Trichinella papuae
AEE50369  159 AYDRKLNARALEIAraq--gvaAHEGVYLGLQGPNLETPAEYNFAHLMGaDVVGMSTVPEVLVARHMDLPLLVVSIATNR 236 Haliscomenobacter ...
OAV43561  159 AYDRDLNQWAIERVadh--glkGHEGVYLALPGPNLETPAEYRMANIIGaDCIGMSTVPEVLVARHCGLPVFVISVVSNK 236 Lewinella sp. 4G2
Feature 1     #         # #  #                   
1B8O_A    245 VImdyesqGKANHEEVLEAGKQAAQKLEQFVSLLM 279 cow
3F8W_A    247 SVldvesdLKPNHEEVLATGAQRAELMQSWFEKII 281 Schistosoma mansoni
ERL87845  604 CVdsyeeaEEPNHAHILDVVQAKAEKLKNLVIKFV 638 mountain pine beetle
KFD51774  236 YGky---lQKRVDYDTYLKFKQSSRDLLCIGEKFI 267 pig whipworm
KFD52565  266 LGdy--sgKSTLHQEVVEVGKKVATDLMPILEGVC 298 pig whipworm
KFD54845  241 MGth---lQKSGADEVTKTIEESNMNLLGFTEQFI 272 pig whipworm
KRZ14634  256 IRe-----NNEQEKFQDTANQQEKATLFQFIEQFC 285 Trichinella zimbabwensis
KRZ68593  288 SEdn--scKTTLHDDVLAIGKQVCRHLVPIFEKFI 320 Trichinella papuae
AEE50369  237 CFpie-diRETSLDDVVAVAKGAEKRLSGIIKALL 270 Haliscomenobacter hydrossis DSM 1100
OAV43561  237 CFpia-eiQPTTVQDVIDTVQAVEPKLRGFVKDLV 270 Lewinella sp. 4G2

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