Conserved Protein Domain Family
CoV_nucleocap

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pfam00937: CoV_nucleocap 
Coronavirus nucleocapsid
Coronavirus (CoV) nucleocapsid (N) proteins have 3 highly conserved domains. The N-terminal domain (NTD) (N1b), the C-terminal domain (CTD)(N2b) and the N3 region. The N1b and N2b domains from SARS CoV, infectious bronchitis virus (IBV), human CoV 229E and mouse hepatic virus (MHV) display similar topological organizations. N proteins form dimers, which are asymmetrically arranged into octamers via their N2b domains. Domains N1b and N2b are linked by another domain N2a that contains an SR-rich region in which phosphorylation of specific serine residues allows the N protein to associate with the RNA helicase DDX1 permitting template read-through, and enabling the transition from discontinuous transcription of subgenomic mRNAs (sgmRNAs) to continuous synthesis of longer sgmRNAs and genomic RNA (gRNA). It has been shown that N proteins interact with nonstructural protein 3 (NSP3) and thus are recruited to the replication-transcription complexes (RTCs).
Statistics
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PSSM-Id: 460005
Aligned: 25 rows
Threshold Bit Score: 293.488
Created: 21-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
AIA62269      13 AARGRSGRVPFSFYMPVINNSSQPFYKVMPQNAVPKGQG-NKDQQIGYWNEQVR--WRMVKGTRKDLPSKWHFYYLGTGP 89 
YP_009113031  49 QASGGNVVPFYSWFSGITQFQKGQEFGFEQGQGVPLTNGvPPSEEKGYWYRHNRksYRTADGQQRQLLPRWYFYYLGTGP 128
YP_001876448  26 QAPALPTSGNVSWFQAIKGRKAGEEGITFKGPGVPVNENvSKNQNHGYWIQRSR--TTPGG---KKLAPLYYFYYTGTGP 100
YP_005352850   1 MATPSVPTTDASWFQVLKAQNKKATHPQFRGNGVPLNSAiKPAENHGYWLRYTR--QKPGGT---QIPPSYAFYYTGTGP 75 
AFD29248       1 MATPTVPVADASWFQPLKAQNKKNTTPSFKGDGVPINTSlDHKAAHGYWLRFNR--QKPGGA---QLPPSFAFYYTGTGP 75 
YP_005352875   1 MAVPSVPKADCSWFQIIKAQNKKVQPLAFKGDGVPINTTlPKEMCHGYWLRYKR--VKPGGA---PLPPSYSFYYTGTGP 75 
AOG30826      50 EKPRAAPMQNVSWFAPLVQTGK-SDLKFAHGEGVPVSQGvDGTYQHGYWLRTQR--NFTKGGKSLTANPRWYFYYTGTGR 126
YP_009072446  35 RKPRPAPQNNVSWFTPLTQHGK-QALTFNPGQGVPLNANdDPDSRIGYWRRNIR--TVTKNGKPQKLDPRWYFYYLGTGP 111
AGX27807      31 PKPRPAPNVSVSWYTGITQHGK-QPLAFPAGQGVPLNANsTPKQNAGYWRRQDRk-LNTGNG-VKQLAPRWYFYYTGTGP 107
A3EXA1        31 PKPRPAPNNTVSWYTGLTQHGK-NPLAFPPGQGVPLNANsTTAQNAGYWRRQDRk-INTGNG-VKQLAPRWFFYYTGTGP 107 Tylonycteris ba...
AIA62269      90 HADLKFRQRQ--------QGVFWVAKEGAKAEPTG-LGTRGR---NAELTTPIFNPG--LPDSIEIV-DQYsrp--nsRA 152
YP_009113031 129 YANKQYGSDI--------DGVVWVASPNVDVRSPAdVPDRDPt--SHQAEPTRFAPGtvLPKGYYVE-GSG-------RS 190
YP_001876448 101 RDQAKYGQEI--------PYVTWVKGDGAKTSQLSeAGSRDVn-kFPNAFPLIFPTGdgPERGFFIErSRS-------RS 164
YP_005352850  76 RGNLKYGELPpndt-qatTRVTWVKGPGADTSIKPhVAKRNPnnpKHQLLPLRFPTGdgPAQGF--------------RV 140
AFD29248      76 RGNLKYGEFPpnes-kgnSRITWVHVKDADTSIKPkVAKRNPqnpKHALLPLRFPPGdgPAQGF--------------RI 140
YP_005352875  76 RATLKYGELQdsdnqdnvNRVLWVKSPDADTSIKPkVAKRNPd--KHPLLPLRFKPGdgPVQGF--------------RI 139
AOG30826     127 YADLRFGTRQ--------PDILWVGRNGANVNKVGnMGTRNPn--NDSAIPVQLPEG--IPKGFYAE-GT--------RS 185
YP_009072446 112 EQNLKYLQQK--------DGIVWVAAHGALNVPVKtVGTRNPa--NDQAIVAAFAPGteLPKGFYVE-GSRsts--saSS 178
AGX27807     108 EANLPFRTVK--------DGIYWVWEEGASEAPSD-FGTRNPa--NDAAIVTQFAPGtqLPKNCHIE-GTGgnsqsssRA 175
A3EXA1       108 EANLPFRSVK--------DGIVWVYEEGATDAPSV-FGTRNPa--NDAAIVCQFAPGtlIPKNFHIE-GTGgnsqsssRA 175 Tylonycteris ba...
AIA62269     153 SSRARSQSndqgnRSRSQsnn--raqSNNRSQSRGRQNQNqnnqptgdggsngqrnqprnrsnsrnrsgnqgrnggsqqd 230
YP_009113031 191 APSSRSSS-----RASSRass--vggSRSRNNSASRNNTSe--------------------------------------- 224
YP_001876448 165 RSNSQADN-----RSKSRers--qssDKPRKEDRGRSKSP---------------------------------------- 197
YP_005352850 141 DPFN-ARG-----RPQERg-------SGPRSQSANPRGTN---------------------------------------- 167
AFD29248     141 DPFQPQRG-----RGMERg-------PTQRSQSANPRQND---------------------------------------- 168
YP_005352875 140 DPF----------QSRGR--------SVERGQQDRRAQSA---------------------------------------- 161
AOG30826     186 RNSSRSSS-----RGSSRntsasnsrANSRASSPGRNLPPqag----------------------------------sep 226
YP_009072446 179 AASSRSNS-----RSRNSsls--rssSPGRGLPPATDPNGv--------------------------------------- 212
AGX27807     176 SSASRNSS-----RSNSRgs-----qPGSRSHSPGSVGPSd--------------------------------------a 207
A3EXA1       176 SSNSRNSS-----RSSSRggr---stSNSRGTSPVSHGVGsae----------------------------------sla 213 Tylonycteris ba...
AIA62269     231 LVAAVREALAGMGFKPNTSGsGRNTPVKVPKGDKPLNKptkapASQVEKPVWKRTPHSQeNVEVCFGPRDTYQ---NFGD 307
YP_009113031 225 VTPDMADQIASLVLAKLNKE--SGKPVQVTKQTAKEIK-----QKILNKPRQKRTPNKQcTVQQCFGKRGPSQ---NFGG 294
YP_001876448 198 ------------AQKNPGSG------GKITKEKAALMV---------SRAYSKRTLPPNkPVDQVFGSRDTEK---NFGD 247
YP_005352850 168 -----------------------NQPRKRDQSAPAAVR------RKTQHQAPKRTLPKGkTISQVFGNRSRTGa--NVGS 216
AFD29248     169 -----------------------AGNRRRAASAPGTNR-----SKTHTHAIPKRTLQKGkTISAVFGKRSPAGs--NVGS 218
YP_005352875 162 ---------------------EPQRPKQRERSRSAPSR-----AAPKQHAIPKRVLLKGkTISGTFGKRSPAKa--NVGS 213
AOG30826     227 WMAYLVQKLQALESKVDGKK-PEKTPQKVTKQGAANMA---------DKLRHKRTPHKGsGVTQNFGRRGPGDlegNFGD 296
YP_009072446 213 LAALLLTKLEALDAKVNGPK----QPPVVTKKTAAEIA---------AKPRQKRVAHKGyNVNAAYGRRGPGPyqgNFGT 279
AGX27807     208 SALLYLELAKRLEALEAGKS--KSAPKVITKKDAADAK---------KKMRHKRVATKAyNPTQAFGLRGPGDlqgNFGD 276
A3EXA1       214 ALPLLLDLQKRLADLESGKS---KQPKVVTKKDAAAAK---------NKMRHKRVATKGfNVTQAFGLRGPGPlqgNFGD 281 Tylonycteris ba...
AIA62269     308 SQLVRLGVDYPHYPQIGELIPSQAALLFGSEITAH---------ERGD-NI------QLTYIYKMEVPKDHKSLAAFLPH 371
YP_009113031 295 GSMLKLGTSDPQFPILAELAPTAGAFFFGSKLELAkvnnmsgaaDEPQkDVy-----ELRYSGAIRFDSTLPGFETIMKV 369
YP_001876448 248 AEMIKKGMSDPRFTAALIPTPGPQAALFGSHVTLK---------EKPD-GL------EITYKYRTLFPKTDERYEEVKHA 311
YP_005352850 217 ADTEKTGMADPRIMALARHVPGVQEMLFAGHLESN---------FQAG-AI------TLTFSYSITVKEGSPDYERLKDA 280
AFD29248     219 ADTEKAGMADGRIMALARQVPGVQEMLFAGHLSHQ---------FQAD-AV------TLTWTYSITIKSDSSDYDRIKQA 282
YP_005352875 214 PDTEKTGLGDPRLMALMRFVPGTQELLFAGHLKHK---------MQPE-GV------QLTFTYDITVKRDNKDFDRIVEA 277
AOG30826     297 LEMLKLGTDDPRFPAAAQMAPTAAAFLFMSHFDTR---------EEND-AT------WLNYRGAIKLPKDNPNYATWMKL 360
YP_009072446 280 QEFNKLGTDYPKWPQIAQLAPTPSAFFGMSRFAVQ---------KNDD-GT------WLTYHGHIKMDESDPNFQVWMTE 343
AGX27807     277 LKYCKEGVDDPRWPQMAELAPSASAFLSMSQLKLV---------HHSN-DTdnkpvyMLRYSGAIKLDPKNPNYSKWLEI 346
A3EXA1       282 MNYNKFGTEDPRWPQMAELAPSASAFMSMSQFKLT---------HQSN-DDkgdpiyFLSYSGAIKLDPKNPNYKKWLEL 351 Tylonycteris ba...
AIA62269     372 IGAYADSTed------vTLPPALPPKQQRLR---------------RSASTEVLADTTS 409
YP_009113031 370 MNENLDAYqhte--egaQLLSPKFQRKQKKG---------------QVEKDNVSAATPK 411
YP_001876448 312 YLSNVPLQ-----------DGGQPLHSSGVQkklk-------srskSRDRTDDGVENPT 352
YP_005352850 281 LNTVVNQT---------YEPPTKPTKDKKPEkq------------dQSAKPKQQKKPKK 318
AFD29248     283 LDTVVDQT---------YEAPIKEKKQPAKQqp-----------kqDKPKPQRSPKKQD 321
YP_005352875 278 LNSVVDMVyepegeirkDQQSKKDTQPKKKA---------------EKKTPTNPQKQPD 321
AOG30826     361 LKENVDAY---------KDFPQTPERETKKK---------------KKDVVVEDVTVVE 395
YP_009072446 344 LQQNIDAY---------KNFPQKEEKKS------------------RKPKTKNVDMAPQ 375
AGX27807     347 LEGNIDAY---------KNFPKKEKKQKQSQikqeaedpewdstdlFSQPLQDPDNQPK 396
A3EXA1       352 LESNIDAY---------KTFPKKERKPKTTEdgavas----ssasqMEDVDAKPQRKPK 397 Tylonycteris bat coronavirus HKU4
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