1J7U,4FEV,1L8T,1ND4,4FEU,4EJ7


Conserved Protein Domain Family
APH

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cd05150: APH 
Click on image for an interactive view with Cn3D
Aminoglycoside 3'-phosphotransferase
APH catalyzes the transfer of the gamma-phosphoryl group from ATP to aminoglycoside antibiotics such as kanamycin, streptomycin, neomycin, and gentamicin, among others. The aminoglycoside antibiotics target the 30S ribosome and promote miscoding, leading to the production of defective proteins which insert into the bacterial membrane, resulting in membrane damage and the ultimate demise of the bacterium. Phosphorylation of the aminoglycoside antibiotics results in their inactivation, leading to bacterial antibiotic resistance. The APH gene is found on transposons and plasmids and is thought to have originated as a self-defense mechanism used by microorganisms that produce the antibiotics. The APH subfamily is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).
Statistics
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PSSM-Id: 270699
Aligned: 79 rows
Threshold Bit Score: 176.618
Created: 26-Sep-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 30 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1L8T; Enterococcus faecalis APH type IIIa binds kanamycin, ADP and Mg2+; defined at 4A contacts.
  • Structure:4FEV: Acinetobacter baumannii APH(3')-Ia binds inhibitor and substrate kanamycin; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          # ###    #           # #                             #               ####  
1J7U_B     17 YRCVKDTEGMsPAKVYKLVGen-eNLYLKMTDsrykgttydVEREKDMMLWLEGklPVPKVLHFErhdgwSNLLMSEADG 95  Enterococcus faecalis
1L8T_A     16 YRCVKDTEGMsPAKVYKLVGen-eNLYLKMTDsrykgttydVEREKDMMLWLEGklPVPKVLHFErhdgwSNLLMSEADG 94  Enterococcus faecalis
EHI70209   23 DSMIYDSSSStAACVFFIEKd--qGYFLKTAPkg------kLKREFEMTCFFHKkgLAPEVIDYQsl-drDWLLTQRVPG 93  Streptococcus icta...
AAB81906   24 DAKIYDSSSSpEAQVLFIDKk--dGYYLKIASsk------tLEREAEMTAYFQKkkLGLGYISYLsdqsqDFLLKKKIQG 95  Lactococcus lactis
CAW95005   23 NSLVYDSSSSaEARVYAIDKg--qGYFLKTAAkg------rLAKEAALTRYCHSkgLATEVLDYIsg-dqDWLLTAKVAG 93  Streptococcus equi...
CCI85668   23 QAKTYDSSSSpEARTYFIKRd--rGYFLKVAAtd------sLKDEATMDDYFYKkkIGPEVVEYLsl-dqDYLLTRQVPG 93  Lactobacillus past...
NP_965495  23 GSPIYNSSSSpEARVYFVDKd--eGYYLKRARlg------kLEKEAKMTQYFYSkgLSAEVLDYTan-dyDWLLTRAVKG 93  Lactobacillus john...
ACZ07253   18 MFFTRIDTGEsGAKVFKVSSdt-aSYYLKIETaa-----geLEEEYQKTRWLQGklEVPDILYFGeenknKYMLLTEING 91  Sebaldella termiti...
P00553     19 TELTVNKIGYsGDHVYHVKEyrgtPAFLKIAPsvw---wrtLRPEIEALAWLDGklPVPKILYTAehggmDYLLMEALGG 95  Bacillus circulans
ADL34965   20 REYTIDDMGKsGSKVLIFEDm---VLKITDKPc-------dDKDAVEMMRWLEGkiPAPQVIVFEeddsySYLLMTKVRG 89  Butyrivibrio prote...
Feature 1      #                                                           #####              
1J7U_B     96 VLCSeeyedeqspEKIIELYAECIRLFHSIDIs-dCPYTNSldsRLAELDYLLNndladvdCENWEEdtp----fkdPRE 170 Enterococcus faecalis
1L8T_A     95 VLCSeeyedeqspEKIIELYAECIRLFHSIDIs-dCPYTNSldsRLAELDYLLNndladvdCENWEEdtp----fkdPRE 169 Enterococcus faecalis
EHI70209   94 EGANqiey-lrqpKKLINIVVEQLQKLHELHFteiSAINRTd-sYIQTVELNYQkgv--fnPNFLSSrdl----fktSYD 165 Streptococcus icta...
AAB81906   96 ENYLakqy-lnnpKRLCDNLAENLRFLHEQNFe-dCPILDHserYLKKVEKNASinn--snLDFVNNyni-----rtTEE 166 Lactococcus lactis
CAW95005   94 EDAShiey-lsdpKRLCRILVDHMLKLHSLSLs-dFPVADKtlaYIEAAEAGYHqgr--fnQCFLPPqqr----fqsQEE 165 Streptococcus equi...
CCI85668   94 YDCVqaey-ldqpERLVKLLAENLAWLHQLDFq-gCPITRTs-nYLEKALDNFEkgh--ydSNFLAEssf-----sdIES 163 Lactobacillus past...
NP_965495  94 EDCVysey-lanpERLCDAIACELRRLHETDYt-dCPVMEKtseYLATTEKNYQtgn-ydkSAFPDSfgy-----rsAEE 165 Lactobacillus john...
ACZ07253   92 RILCdkeh-irnpEAAIKLLAEGLVKLGGVDTd-sCPFDNRlekKLEKAAENVRlnr-vnmTDWEETtkf-----snPES 163 Sebaldella termiti...
P00553     96 KDGSheti-qakrKLFVKLYAEGLRSVHGLDIr-eCPLSNGlekKLRDAKRIVDesl-vdpADIKEEyd------ctPEE 166 Bacillus circulans
ADL34965   90 KVACdkyy-lerpKELVPLLSKSIKMLQSIDIt-dCPVVKNvdrELKKAAYRVKngl-vdiSDAEPDtfgengrfrdPEE 166 Butyrivibrio prote...
Feature 1                         #   ## #         ##                 ##  #                   
1J7U_B    171 LYDFLKTekp-eeELVFSHGDLGDSNIFVkdgkVSGFIDLGRSGRadKWYDIAFCVRSIREDig-------eEQYVELFF 242 Enterococcus faecalis
1L8T_A    170 LYDFLKTekp-eeELVFSHGDLGDSNIFVkdgkVSGFIDLGRSGRadKWYDIAFCVRSIREDig-------eEQYVELFF 241 Enterococcus faecalis
EHI70209  166 IFDYIQEikplleSDTLIHGDFCLPNVILnnwkFQSMIDWDCSGMgdKHIDLFWLLWTFQHQlk-------tATFKDYIL 238 Streptococcus icta...
AAB81906  167 AYDYIENkklllrNDTLLHGDYCLPNIILdnwkFKGFIDLDCAGVgdRHIDLFWGAWTLNFNig-------tDQYRDRFF 239 Lactococcus lactis
CAW95005  166 AYRRIQElklllqHETLIHGDFCLPNMILdrwqFQSMIDWGEAGKsdRHIDLFWLIWSLQFNlk-------tDRYKDYVL 238 Streptococcus equi...
CCI85668  164 AHDYILAhrdwlkADILIHGDFCQPNIILdnwnFSGYIDLDCAGVgdRHVDIYWALHSLKFNfk-------tDKYASLFI 236 Lactobacillus past...
NP_965495 166 AHQVLEAgkdalqDKVLLHGDYCLPNIILdnwkFSGFIDVDCSGVgdRHIDLFWGIWTLWFNlk-------tNKYRDRFL 238 Lactobacillus john...
ACZ07253  164 LLNYLYKnrppenEVIFTHGDYCLPNIIAeldeITGFIDLGRAGIadIWQDIALCMRSMHRNfg-------tRKYEELLL 236 Sebaldella termiti...
P00553    167 LYGLLLEskpvteDLVFAHGDYCAPNLIIdgekLSGFIDLGRAGVadRYQDISLAIRSLRHDyg-------dDRYKALFL 239 Bacillus circulans
ADL34965  167 LLSWLQEnrp-pyEPVLSHGDLCLPNILIekgnISGFIDMGNCGIadKWEDIAILYRSLRHNfdgtygkiypGLEPDSFF 245 Butyrivibrio prote...
Feature 1                        ## #
1J7U_B    243 DLLGik-pDWEKIKYYILLDELF 264 Enterococcus faecalis
1L8T_A    242 DLLGik-pDWEKIKYYILLDELF 263 Enterococcus faecalis
EHI70209  239 DCYGreaiEMSILEAIPYFEVFG 261 Streptococcus ictaluri 707-05
AAB81906  240 DAYGrdriDVDRLKLVGCCEVFG 262 Lactococcus lactis
CAW95005  239 DCYGrrhiDLDILDGIAAFEVFA 261 Streptococcus equi subsp. equi 4047
CCI85668  237 DTYGrdkvDFKRLKLIDAIEIFG 259 Lactobacillus pasteurii CRBIP 24.76
NP_965495 239 DAYGrdkvDEALLDVIAAAEVFG 261 Lactobacillus johnsonii NCC 533
ACZ07253  237 EYLGrk-qDKEKLEYYILLDEMF 258 Sebaldella termitidis ATCC 33386
P00553    240 ELYGldglDEDKVRYYIRLDEFF 262 Bacillus circulans
ADL34965  246 EELGie-pDREKIDYYILLDELF 267 Butyrivibrio proteoclasticus B316

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