RT_ZFREV_like: A subfamily of reverse transcriptases (RTs) found in sequences similar to the intact endogenous retrovirus ZFERV from zebrafish and to Moloney murine leukemia virus RT. An RT gene is usually indicative of a mobile element such as a retrotransposon or retrovirus. RTs occur in a variety of mobile elements, including retrotransposons, retroviruses, group II introns, bacterial msDNAs, hepadnaviruses, and caulimoviruses. These elements can be divided into two major groups. One group contains retroviruses and DNA viruses whose propagation involves an RNA intermediate. They are grouped together with transposable elements containing long terminal repeats (LTRs). The other group, also called poly(A)-type retrotransposons, contain fungal mitochondrial introns and transposable elements that lack LTRs. Phylogenetic analysis suggests that ZFERV belongs to a distinct group of retroviruses.
Feature 1:DNA binding site [nucleic acid binding site]
Evidence:
Comment:Determined from interactions of the MMLV RT fragment with DNA duplexes in three distinct crystal lattices. For some of these active residues there is support from site-directed mutagenesis studies.
Structure:1QAJ_A; Moloney murine leukemia virus (MMLV ) N-terminal fragment RT binds an 8-mer DNA duplex (5'-CATGCATG-3'); contacts at 3.5A.
Comment:Conserved residues on a finger domain is responsible for DNA binding.
Structure:1D0E _A; Moloney murine leukemia virus (MMLV ) N-terminal fragment RT binds an 11-mer DNA duplex (5'-TTTCATGCATG-3'); contacts at 3.5A.
Jiyao W et al.(2023). "The conserved domain database in 2023.",