2E8A,1HPM,1NGC,1S3X,5FPN,2V7Z,3A8Y,3C7N,3CQX,2QWN,3FZF,3I33,3KVG,3D2E,5BPM,4IO8,6RZQ


Conserved Protein Domain Family
ASKHA_NBD_HSP70_HSPA1

?
cd10233: ASKHA_NBD_HSP70_HSPA1 
nucleotide-binding domain (NBD) of 70-kDa heat shock protein 1 (HSPA1) and similar proteins
This subfamily includes human HSPA1A (70-kDa heat shock protein 1A, also known as HSP72; HSPA1; HSP70I; HSPA1B; HSP70-1; HSP70-1A), HSPA1B (70-kDa heat shock protein 1B, also known as HSPA1A; HSP70-2; HSP70-1B), and HSPA1L (70-kDa heat shock protein 1-like, also known as HSP70T; hum70t; HSP70-1L; HSP70-HOM), HSPA2 (70-kDa heat shock protein 2, also known as HSP70-2; HSP70-3), HSPA6 (also known as heat shock 70kDa protein 6; HSP70B'), HSPA7 (heat shock 70kDa protein 7 , also known as HSP70B), and HSPA8 (heat shock 70kDa protein 8, also known as Lipopolysaccharide-associated protein 1/LAP1; HSC70; HSP73; HSPA10). They are molecular chaperones implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. They play pivotal roles in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The subfamily also includes Saccharomyces cerevisiae heat shock protein Ssa1-4, which may play a role in the transport of polypeptides both across the mitochondrial membranes and into the endoplasmic reticulum. This subfamily belongs to the heat shock protein 70 (HSP70) family of chaperones that assist in protein folding and assembly and can direct incompetent "client" proteins towards degradation. Typically, HSP70s have a nucleotide-binding domain (NBD) and a substrate-binding domain (SBD). The nucleotide sits in a deep cleft formed between the two lobes of the NBD. The two subdomains of each lobe change conformation between ATP-bound, ADP-bound, and nucleotide-free states. ATP binding opens up the substrate-binding site; substrate-binding increases the rate of ATP hydrolysis. HSP70 chaperone activity is regulated by various co-chaperones: J-domain proteins and nucleotide exchange factors (NEFs).
Statistics
?
PSSM-Id: 466831
Aligned: 103 rows
Threshold Bit Score: 777.962
Created: 6-Oct-2006
Updated: 27-Apr-2023
Structure
?
Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 23 residues -Click on image for an interactive view with Cn3D
Feature 1:nucleotide binding site [chemical binding site]
Evidence:
  • Structure:2V7Z; Rat Hsc70-NBD binds hydrolyzed ATP (ADP-Pi), contacts at 4A
  • Structure:2E8A; Human heat shock 70 kDa protein 1A/1B-NBD binds MgAMPPNP, contacts at 4A
  • Structure:1S3X; Human heat shock 70 kDa protein 1A/1B-NBD binds ADP, contacts at 4A
  • Citation:PMID 9083109
  • Structure:3I33; Human 70kDa heat shock protein 2 binds MgADP-Pi ; contacts at 4A

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1         # ####                                                                      
2E8A_A      9 AIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQvALNPQNTVFDAKRLIGRKFgDPVVQS 88  human
2V7Z_A      6 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQvAMNPTNTVFDAKRLIGRRFdDAVVQS 85  Norway rat
AAA19122    6 AVGIDLGTTYSCVGVYQNEKVEIIANEQGAYTTPSYVAFTDADGLIGDSAKNQcALNPENTIFDAKRLIGRRFnDPEVQA 85  Giardia intestinalis
BAA97566    9 SVGIDLGTTYSCVGVWQNDKVEIIANDQGQRTTPSYVAFTDTERLIGDAAKNQvAMNPTNTVFDAKRLIGRKFdDPSVQS 88  Blastocystis hominis
AAQ24867    6 AIGIDLGTTYSCVGVYMGDNVEIIPNDQGNRTTPSYVAFTDDERLIGDAAKNQvAMNPHNTVFDAKRLIGRKFsDTSVQN 85  Naegleria gruberi
AAW22172    7 AVGIDLGTTYSCVGVWRNETVEIIPNDQGNRTTPSWVAFTPTERLIGDAAKNQgNMNPENSLFDSKRMIGRRFtDSEIQN 86  
EED91615    8 SVGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTETERLIGDAAKSQaAMNASNTVFDAKRLIGRKFsDPGVQS 87  Thalassiosira pseu...
GAX99452    8 SVGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQvAMNASNTVFDAKRLIGRKFsDAVVQS 87  Pythium insidiosum
GBG31505    7 GIGIDLGTTYSCVGCWQNDSVEIIANDQGNRTTPSYVAFTSTERLIGDAAKSQvSMNPYNTVFDAKRLIGRKFaDPSTQQ 86  Hondaea fermentalg...
QBK91737   37 YIGLDCGTTYSAVGIFKNGKVEIIANDQGNRTTPSYVAFTDTERLVGDPAKNQcSMNPENTVFDAKRLIGRKYsDPVVQS 116 Pithovirus LCPAC304
Feature 1                                                                                     
2E8A_A     89 DMKHWPFQVINDg-DKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGypVTNAVITVPAYFNDSQRQATKDAGVI 167 human
2V7Z_A     86 DMKHWPFMVVNDa-GRPKVQVEYKGETKSFYPEEVSSMVLTKMKEIAEAYLGktVTNAVVTVPAYFNDSQRQAAKDAGTI 164 Norway rat
AAA19122   86 DLKHFRSRSSCGptRTPQIQVVYKGETKTFTPEEISSMVLTKMKDIASDYLGnkVTEAIVTVPAYFSDAHGKATQNAGTI 165 Giardia intestinalis
BAA97566   89 DMKHWPFTLKAGpdGKPLIEVEYKGEKKTFSPEEISSMVLSKMKQIAEAYLGkeVKNAVITVPAYFNDSQRQATKDAGVI 168 Blastocystis hominis
AAQ24867   86 DIKHWPFKVITKgdDKPYIQVEYKGESHIFTPEQISSMVLTKMKDIAEQYVGkkVQKAVVTVPAYFNDSQRQATKDAGQI 165 Naegleria gruberi
AAW22172   87 DIKHWPFKVTEKd-GKPMYQVKYKGETKEFSPEEISSMVLSKMKEISEAYLGhkVTEAVVTCPAYFNDSQRQATKDAGTI 165 
EED91615   88 DMKHWPFKVIPGtgGTPIIEVEYKGETKQFKAEEISSMVLTKMKEIAEAYLGkeVKNAVVTVPAYFNDSQRQATKDAGAI 167 Thalassiosira pseu...
GAX99452   88 DMKHWPFKVVAGsgDKPQIVVEFKGERKTFQPEEISSMVLTKMKEIAEAYLGttVKNAVITVPAYFNDSQRQATRDAGTI 167 Pythium insidiosum
GBG31505   87 DLKFFPFKVEAGpnDKPVIKVEFQGEEKTFAPEEISSMVLTKMKQTAESYLGeeVKNAVITVPAYFNDSQRQATKDAGVI 166 Hondaea fermentalg...
QBK91737  117 DKKLWPFEVVQHegDKPFIQVTYKSETRAFAPEEITSMILGKMRDIAESYLGesIQGAVITVPAYFNDAQRQATKDAGVI 196 Pithovirus LCPAC304
Feature 1                                        # #### #                         ##          
2E8A_A    168 AGLNVLRIINEPTAAAIAYGLDRtg-kgERNVLIFDLGGGTFDVSILTIDd--GIFEVKATAGDTHlGGEDFDNRLVNHF 244 human
2V7Z_A    165 AGLNVLRIINEPTAAAIAYGLDKkv-raERNVLIFDLGGGTFDVSILTTEd--GIFEVKSTAGDTHlGGEDFDNRMVNHF 241 Norway rat
AAA19122  166 AGLNVLRIINEPTAAAIAYGLDKstskkERNILIFDLGGGTFDVSLLTVDpssGVFEVKATAGDTHlGGEDFDSRVVNYF 245 Giardia intestinalis
BAA97566  169 AGLNVMRIINEPTAAAIAYGLDQkg--gEKNILIFDLGGGTFDVSLLSIEd--GIFEVKATAGDTHlGGEDFDNRMVEYF 244 Blastocystis hominis
AAQ24867  166 AGLEVLRIINEPTAAAIAYGLNKk---dEKYVLIFDLGGGTFDVSILNIEd--GVFEVKATAGDTHlGGEDFDSRMVEYC 240 Naegleria gruberi
AAW22172  166 AGMNVLRIINEPTASAIAYGLDKkk-dtELNVLIFDYGGGTLDVSILCLDs--GVFEVKATAGDIHcGGEDLDNILTHYM 242 
EED91615  168 SGLNVLRIINEPTAAAIAYGLDQkg--eEKNVLIFDLGGGTFDVSLLTIEe--GIFEVKATAGDTHlGGEDFDNRMVDYF 243 Thalassiosira pseu...
GAX99452  168 AGLNVLRIINEPTAAAIAYGLDKkg--gERNVLIFDLGGGTFDVSLLSIEe--GIFEVKATAGDTHlGGEDFDNRLVDYF 243 Pythium insidiosum
GBG31505  167 AGLNVLRIINEPTAAAIAYGLDKke--gERNVLIFDLGGGTFDVSLLTIEe--GIFEVKATAGDTHlGGEDFDNRLVEHF 242 Hondaea fermentalg...
QBK91737  197 AGLTVKRIINEPTAAAIAYGLEQqg-qgEKNVLIFDCGGGTFDVSLLCIEd--GIFEVKAVSGNSHlGGEDFDNRLVAHF 273 Pithovirus LCPAC304
Feature 1                               #  ##  #                                              
2E8A_A    245 VEEFKRKhkkdisqnKRAVRRLRTACERAKRTLSSsTQASLEIDSlfeGIDFYTSITRARFEELCSDLFRSTLEPVEKAL 324 human
2V7Z_A    242 IAEFKRKhkkdisenKRAVRRLRTACERAKRTLSSsTQASIEIDSlyeGIDFYTSITRARFEELNADLFRGTLDPVEKAL 321 Norway rat
AAA19122  246 IAEFKKKhgkdisgsNRAMRRLRTACEEAKRTLSSsQQASIEIESlfeGIDFFTNITRAKFEDLCIDLFRKCLDPVDRVL 325 Giardia intestinalis
BAA97566  245 CTEFQRKfrkdlttsQRALRRLRTACERAKRTLSSsTQATIEIDSlfeGIDFNSQITRARFEELNMDYFRKTMAPVEKVL 324 Blastocystis hominis
AAQ24867  241 CTEFKRKyrrdvhdnPRALRRLRTACERTKRNLSSaSTSTINVDSivdGIDLDIPMTRAKFEQLNMDMFRNCFEPVKKVL 320 Naegleria gruberi
AAW22172  243 ADEFKRKykkdlmgnSRSVGRLRTACERAKRALSTsTQATIEIDSlfeGLDFNAVITRAKFEQLCESQFRKCLEPLDRAM 322 
EED91615  244 LQDFKRRtkkdmsqnQRSLRRLRTACERAKRTLSSsTQAHIEIDSlfdGVDFNSTITRARFEDLCMDYFKKCMDPCEKVL 323 Thalassiosira pseu...
GAX99452  244 VAEFKRKhrkdltsnQRSLRRLRTACERAKRTLSSsTQAHIEIDSlfdGVDFNSTITRARFEDMCADYFRKTMEPVEKVL 323 Pythium insidiosum
GBG31505  243 MAEFKRKfkkdmssnERAKRRLRTACERAKRTLSAsAQAHIEIDGlfeGVDFTSSISRARFEDLNMDYFKSTMEPVEKVL 322 Hondaea fermentalg...
QBK91737  274 CQEFKRKhkadlytnPRAIRRLRTACERAKRTLSSaTQASVEIDAlhdGEDFYAKITRARFEELCNDLFRSTMNPVDQVL 353 Pithovirus LCPAC304
Feature 1                     ### ##                      #              
2E8A_A    325 RDAKLDKaQIHDLVLVGGSTRIPKVQKLLQDFFNGRDlnKSINPDEAVAYGAAVQAAIL 383 human
2V7Z_A    322 RDAKLDKsQIHDIVLVGGSTRIPKIQKLLQDFFNGKElnKSINPDEAVAYGAAVQAAIL 380 Norway rat
AAA19122  326 RDSKLGKnDVHDIVLVGGSTRIPKVQQMLRDFFSGRElsKNVNPDEAVAYGASVQAALL 384 Giardia intestinalis
BAA97566  325 RDAKLSKsEVDEVVLVGGSSRIPKVQKLLQEFFNGKElcQSINPDEAVAYGATVQAAVL 383 Blastocystis hominis
AAQ24867  321 QDSGLDKaQIDDVVLVGGSTRIPKVQEMLKEFFNGKElcKSINPDEAVAYGAAVQAGVL 379 Naegleria gruberi
AAW22172  323 LDSKLDKsKINEIVLVGGSTRVPRIQQLIRDRFGGKElcKSINPDEAVAYGAAVQAAIL 381 
EED91615  324 RDAKIAKnQVDEVVLVGGSTRIPKIQSMLAEFFNGKEpnKGINPDEAVAYGATVQAAIL 382 Thalassiosira pseudonana CCMP1335
GAX99452  324 VDAKISKsQVHEVVLVGGSTRIPKVQQLLSDFFNGKEpcKSINPDEAVAFGAAVQAAIL 382 Pythium insidiosum
GBG31505  323 RDAKMSKgSVHEIVLVGGSTRIPKVQAMLKEFFNGKEpcRSINPDEAVAYGATVQAAIL 381 Hondaea fermentalgiana
QBK91737  354 KDSGMDKkSVDEIVLVGGSTRIPKIQQLLSSFFNGKElcKSINPDEAVAYGAAVQAGVI 412 Pithovirus LCPAC304

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap