2A19,2A1A


Conserved Protein Domain Family
STKc_EIF2AK2_PKR

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cd14047: STKc_EIF2AK2_PKR 
Click on image for an interactive view with Cn3D
Catalytic domain of the Serine/Threonine kinase, eukaryotic translation Initiation Factor 2-Alpha Kinase 2 or Protein Kinase regulated by RNA
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. PKR (or EIF2AK2) contains an N-terminal double-stranded RNA (dsRNA) binding domain and a C-terminal catalytic kinase domain. It is activated by dsRNA, which is produced as a replication intermediate in virally infected cells. It plays a key role in mediating innate immune responses to viral infection. PKR is also directly activated by PACT (protein activator of PKR) and heparin, and is inhibited by viral proteins and RNAs. PKR also regulates transcription and signal transduction in diseased cells, playing roles in tumorigenesis and neurodegenerative diseases. EIF2AKs phosphorylate the alpha subunit of eIF-2, resulting in the downregulation of protein synthesis. The PKR subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 270949
Aligned: 5 rows
Threshold Bit Score: 444.241
Created: 10-Apr-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 27 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:includes ATP binding and eIF2alpha (substrate) binding sites
  • Structure:2A19_B; Human PKR kinase domain binds AMP-PNP and eIF2alpha substrate; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                        # #            # #                                           
2A19_B      6 DKRFGMDFKEIELIGSGGFGQVFKAKHriDGKTYVIKRVKYNnEKAEREVKALAKLDHVNIVHYNGCWDGFDYdpetssk 85  human
2A1A_B      6 DKRFGMDFKEIELIGSGGFGQVFKAKHriDGKTYVIKRVKYNnEKAEREVKALAKLDHVNIVHYNGCWDGFDYdpetssk 85  human
CAH68529  163 VSGMSQTFDVIEELGDGGYGFVCKVKHkiDDKIYAVKRVEFN-SEAEPEVKALARLDHPNIVRYFTCWPDPDNwmsnqet 241 zebrafish
NP_989818 272 DERFRQQYKNIEPIGKGGFGNVFKATSrtDERTYAIKRVELInRNVKREVKELANLEHENIVRYYCSWEGTDHmiypdss 351 chicken
CAM07152  294 NEAFRQNFTEISRLGSGGFGTVFKAKSklANKYYAIKRVKLHsNKCIKEVEALAHLDHPNIVRYHHSWTGEDGcsdssys 373 African clawed frog
Feature 1                                                                                #### 
2A19_B     86 n--------------------------------------------------------------ssrsktkCLFIQMEFCD 103 human
2A1A_B     86 n--------------------------------------------------------------ssrsktkCLFIQMEFCD 103 human
CAH68529  242 neksnttgsstdtddtnersasdddvaddeddddddvsddvtlgmesltftestsaakasgqcdptnhrtYLFIQMEFCE 321 zebrafish
NP_989818 352 k--------------------------------------------------------------nsivavsCLFIQMELCE 369 chicken
CAM07152  374 srd---------------------------------------------------------ysspaglkeeYLFIGMEWCE 396 African clawed frog
Feature 1       #     #  #                                     ## #          #                
2A19_B    104 KGTLEQWIEKRRge--kLDKVLALELFEQITKGVDYIHSKKLINRDLKPSNIFLVdtKQVKIGDFGLVTSLKn-----DG 176 human
2A1A_B    104 KGTLEQWIEKRRge--kLDKVLALELFEQITKGVDYIHSKKLINRDLKPSNIFLVdtKQVKIGDFGLVTSLKn-----DG 176 human
CAH68529  322 GGTLTSWIGERNyggkqRIPLEIHRIFYEISSGVEYIHSNNLIHRDLKPDNILFNd-GKVKIGDFGLVAAQKnps-gdPI 399 zebrafish
NP_989818 370 QGPLEKWIENNGgn--pNYHMMAQDKFLQILKGVEYIHSKDLIHRDLKPQNIFLSyeGKIKIGDFGLVTSVT------YN 441 chicken
CAM07152  397 KGTLESWIARMNk----VEKFKSLVIFRQVIEGVVYIHSKGLIHRDLKPANIFFAedLKIKIGDFGLVTQMTgeadrqAL 472 African clawed frog
Feature 1            ###                              # ########                              
2A19_B    177 KRXRSKGTLRYMSPEQIssqDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRdGIISDIFd---KKEKTLLQKLLS 253 human
2A1A_B    177 KRXRSKGTLRYMSPEQIssqDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRdGIISDIFd---KKEKTLLQKLLS 253 human
CAH68529  400 ERSKRKGTPTYMSPEQEnlrNYDEKTDIFPLGLMWFEMLWKISSGMERAEIWTNLRnQKLPDQFceryPAENKFIEKMLS 479 zebrafish
NP_989818 442 PLTKNRGTQSYMAPEQFg-dRYGKEVDIYALGLIWFEILSALVSHHEKNKVWQDVRgGDLPLNFtkrfKIQVPIIKKMLS 520 chicken
CAM07152  473 QRTKGRGTRSYMAPEQHe-eTYESEVDIFPLGLILVELFYIFKDVNAKKEEWGKLRnGELPPGFvkqyHIEEAIIKKMLS 551 African clawed frog
Feature 1                     
2A19_B    254 KkPEDRPNtSEILRTL 269 human
2A1A_B    254 KkPEDRPNtSEILRTL 269 human
CAH68529  480 VtPEDRPHaKDIRLNL 495 zebrafish
NP_989818 521 EdPSKRCSaSQIIDIL 536 chicken
CAM07152  552 RePKRRPKaTYLKKHF 567 African clawed frog

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