2OZO,1U59


Conserved Protein Domain Family
PTKc_Zap-70

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cd05115: PTKc_Zap-70 
Click on image for an interactive view with Cn3D
Catalytic domain of the Protein Tyrosine Kinase, Zeta-chain-associated protein of 70kDa
PTKs catalyze the transfer of the gamma-phosphoryl group from ATP to tyrosine (tyr) residues in protein substrates. Zap-70 is a cytoplasmic (or nonreceptor) PTK containing two Src homology 2 (SH2) domains N-terminal to the catalytic tyr kinase domain. Zap-70 is primarily expressed in T-cells and NK cells, and is a crucial component in T-cell receptor (TCR) signaling. Zap-70 binds the phosphorylated ITAM (immunoreceptor tyr activation motif) sequences of the activated TCR zeta-chain through its SH2 domains, leading to its phosphorylation and activation. It then phosphorylates target proteins, which propagate the signals to downstream pathways. Zap-70 is hardly detected in normal peripheral B-cells, but is present in some B-cell malignancies. It is used as a diagnostic marker for chronic lymphocytic leukemia (CLL) as it is associated with the more aggressive subtype of the disease. The Zap-70 subfamily is part of a larger superfamily that includes the catalytic domains of other kinases such as protein serine/threonine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 270686
Aligned: 5 rows
Threshold Bit Score: 568.808
Created: 15-Jun-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:2OZO; Human Zap-70 tyr kinase binds Mg-ANP; contacts at 4A.
  • Structure:1U59; Human Zap-70 tyr kinase binds the inhibitor, staurosporine; defined at 4A contacts.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                ######  #              # #                                           
2OZO_A    333 KRDNLLIADIELGCGNFGSVRQGVYRMRkKQIDVAIKVLKQGTekadTEEMMREAQIMHQLDNPYIVRLIGVCQaeALML 412 human
AAH77883  321 KRELLLIDEVELGSGNFGCVKKGVYKLKkRQIDVAIKVLKVQEeknvRDEMMKEAEFMHQLDNPYIVRMIGVCEaeNLML 400 African clawed frog
AAH95237  320 KRDKLMIDEVELGSGNFGCVKKGVFKMEsKQIDVAIKVLKNENeksvRDEMMREAEIMHQLSDPFIVRMIGLCEaeALML 399 zebrafish
XP_418206 337 KRDHLMIDEVELGAGNFGCVKKGVYKMRkKQIDVAIKVLKSNNektvRDEMMTEAQIMHQLDNPYIVRMIGVCEaeSLML 416 chicken
1U59_A      7 KRDNLLIADIELGCGNFGSVRQGVYRMRkKQIDVAIKVLKQGTekadTEEMMREAQIMHQLDNPYIVRLIGVCQaeALML 86  human
Feature 1      ##### ##                                           ## #         ##             
2OZO_A    413 VMEMAGGGPLHKFLVGKreeIPVSNVAELLHQVSMGMKYLEEKNFVHRNLAARNVLLVNrHYAKISDFGLSKALGADDSY 492 human
AAH77883  401 VMEMASGGPLNKFLGAKkdtITVSNVVELMHQVSMGMKYLEGKNFVHRDLAARNVLMVNqHYAKISDFGLSKALAADDSY 480 African clawed frog
AAH95237  400 VMEMAPAGPLNKFLSGKkdqITTENIVMLMHQVSMGMKYLEGRNFVHRDLAARNVLLVNqQYAKISDFGLSKALGADDNY 479 zebrafish
XP_418206 417 VMEMASGGPLNKFLSSKkdeITVSNIVELMHQVSMGMKYLEEKNFVHRDLAARNVLLVNqHYAKISDFGLSKALGADDSY 496 chicken
1U59_A     87 VMEMAGGGPLHKFLVGKreeIPVSNVAELLHQVSMGMKYLEEKNFVHRDLAARNVLLVNrHYAKISDFGLSKALGADDSY 166 human
Feature 1                                                                                     
2OZO_A    493 YTARSAGKWPLKWYAPECINFrKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALM 572 human
AAH77883  481 YKAKSFGKWPLKWYAPECINYrKFSSRSDVWSYGITMWEAFSYGQKPYKKLKGTEVMSFIERNERLACPASCPPEMYQLM 560 African clawed frog
AAH95237  480 YKARTGGKWPLKWYAPECIHFhKFSSKSDVWSFGITMWEAFSFGGKPYKKMKGPEVITYIEGGSRLDCPAACPEAMYELM 559 zebrafish
XP_418206 497 YKARTAGKWPLKWYAPECILYhKFSSKSDVWSYGVTMWEAFSYGQKPYKKMKGPEVISFIEQGKRMDCPTECPAEIYALM 576 chicken
1U59_A    167 YTARSAGKWPLKWYAPECINFrKFSSRSDVWSYGVTMWEALSYGQKPYKKMKGPEVMAFIEQGKRMECPPECPPELYALM 246 human
Feature 1                                  
2OZO_A    573 SDCWIYKWEDRPDFLTVEQRMRACYYSLA 601 human
AAH77883  561 LDCWIFKMEDRPNFENVEYRMRMYYYSIA 589 African clawed frog
AAH95237  560 KECWTYKHEERLNFAKVEEKMRTFYYSIA 588 zebrafish
XP_418206 577 MQCWTYSWEERPGFFSVENTIRTYYYSIA 605 chicken
1U59_A    247 SDCWIYKWEDRPDFLTVEQRMRACYYSLA 275 human

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