1W70,2DYB


Conserved Protein Domain Family
SH3_p40phox

?
cd11869: SH3_p40phox 
Click on image for an interactive view with Cn3D
Src Homology 3 domain of the p40phox subunit of NADPH oxidase
p40phox, also called Neutrophil cytosol factor 4 (NCF-4), is a cytosolic subunit of the phagocytic NADPH oxidase complex (also called Nox2 or gp91phox) which plays a crucial role in the cellular response to bacterial infection. NADPH oxidase catalyzes the transfer of electrons from NADPH to oxygen during phagocytosis forming superoxide and reactive oxygen species. p40phox positively regulates NADPH oxidase in both phosphatidylinositol-3-phosphate (PI3P)-dependent and PI3P-independent manner. It contains an N-terminal PX domain, a central SH3 domain, and a C-terminal PB1 domain that interacts with p67phox. The SH3 domain of p40phox binds to canonical polyproline and noncanonical motifs at the C-terminus of p47phox. SH3 domains are protein interaction domains that bind to proline-rich ligands with moderate affinity and selectivity, preferentially to PxxP motifs. They play versatile and diverse roles in the cell including the regulation of enzymes, changing the subcellular localization of signaling pathway components, and mediating the formation of multiprotein complex assemblies.
Statistics
?
PSSM-Id: 212802
Aligned: 6 rows
Threshold Bit Score: 97.179
Created: 31-May-2011
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
peptide ligandPB1 domain
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:peptide ligand binding site [polypeptide binding site]
Evidence:
  • Structure:1W70; Human p40phox SH3 domain binds C-terminal polyproline region of p47phox; contacts at 4A.
  • Comment:SH3 domains bind proline-rich ligands, preferentially to PxxP motifs.
  • Comment:flanking hinge and loops (RT and n-Src) confer sequence specificity for ligand residues outside the core binding motif

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1             ##   #   #              ## ##            #  #    
1W70_A         6 RAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKIL 59  human
2DYB_A       176 RAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKIL 229 human
Q15080       174 RAEALFDFTGNSKLELNFKAGDVIFLLSRINKDWLEGTVRGATGIFPLSFVKIL 227 human
NP_001034361 174 RAEALFDFSGTSKLELSFKKGDLIYLLSRVNKDWLEGTVNDATGIFPSAFVKII 227 chicken
CAK10748      77 RAEAVFDFSGSGRLELSLKAGDVIFLLRRVNADWLEGTVRDRTGIFPESFVKII 130 zebrafish
NP_001089670 171 RAESLFDFKGNASMELNLKCGDLIYLLSWVNREWLEGTVGNRTGIFPASFVRII 224 African clawed frog

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap