3EVB,1R6A,2OXT,2OY0,2PX8,2WA1,3EMD,3GCZ,4CTJ,4K6M,5IKM,5TFR,3ELD,5ZQK,3EVA,4V0R,5CCV,3LKZ,5GOZ,5NJV,5ULP,7D6M


Conserved Protein Domain Family
capping_2-OMTase_Flaviviridae
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cd20761: capping_2-OMTase_Flaviviridae 
Cap-0 specific (nucleoside-2'-O-)-methyltransferase of flaviviridae
Cap-0 specific (nucleoside-2'-O-)-methyltransferase (2'OMTase) catalyzes the methylation of Cap-0 (m7GpppNp) at the 2'-hydroxyl of the ribose of the first nucleotide, using S-adenosyl-L-methionine (AdoMet) as the methyl donor. This reaction is the fourth and last step in mRNA capping, the creation of the stabilizing five-prime cap (5' cap) on mRNA. Flaviviridae viruses, comprise a family of ss(+)RNA viruses, cap their mRNAs. The 2'OMTase activity is located in the non-structural protein 5 (NS5).
Links
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Statistics
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PSSM-Id: 467736
Aligned: 49 rows
Threshold Bit Score: 240.587
Created: 7-Oct-2019
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
Download Cn3D
 
Conserved site includes 11 residues -Click on image for an interactive view with Cn3D
Feature 1:SAM binding site [chemical binding site]
Evidence:
  • Structure:5IKM: Dengue virus 1 NS5 methyltransferase with bound SAM; contacts 4A
  • Structure:3EMD: Wesselsbron virus NS5 methyltransferase domain binds adenosyl-ornithine; contacts at 4A
Download Cn3D for Viewing 3D StructureScroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                       ##
3EVB_A          8 TLGEVWKRELNLLDk----RQFELYKRTdivevdrdtarrhlaegkvdtgvAVSRGTAKLRWFHERgyvklegRVIDLGC 83   Yellow fever ...
5IKM_A         11 TLGEKWKRQLNQLSk----SEFNTYKRSgimevdrseak-eglkrgettkhAVSRGTAKLRWFVERnlvkpegKVIDLGC 85   Dengue virus 1
NP_658908    2518 WRGRQYKFTLNKLNk----HDFLQFKGEnvyel-------------enkddFPSRGFAKMDWMIKStlfqprgFVVDGGC 2580 Tamana bat virus
AKL90417       59 LTYQRYKEFLNSLNy----AQFNAMKMFgvtat-------------rrrfnKASRGYDKLFDLLIIsrlkiygKVLSTCC 121  Guaico Culex ...
YP_009179388   47 DWGPRYKQALNNLGk----KAFDAFKMRgvrpt-------------lrsfnKASRGYDKLMDIMRQtkikpsgRVLSLCC 109  Wuhan aphid v...
YP_009179401   46 RWGGEYKEALRGLGa----RAFNAFKNRgvrrv-------------dkdphVASRGYDKLLEISIAarvkprgAVLSLCC 108  Shuangao inse...
YP_009179405   54 TELPRWAGMISDFSkqlgsQQYDKFKLRginiak------------ktktlASRQGFAKLQDVLMQsrlhpsgFCLNLAA 121  Wuhan cricket...
APG76081       75 DWLAIYKDVVSTMTk----GEFTEFKLRgliey-------------rrnrtMASRGMIKLVDLLNQskvhvygNVLNLCS 137  Changjiang Ji...
AVM87250     2599 KAGKQWADRRNRLSr----TAFAKYKGHntmey--------------srvdEVSRGYAKLEQLEAYarvrilgRIHCHAD 2660 Wenzhou shark...
QBQ65056       64 DLGRKAKVEMNAMTy----DEFDRMKYRgvvae-------------aysgdKPSKGYDKLRVLMDLmdrptlgTTVDLCA 126  Yanggou tick ...

Feature 1         #  ##                   ##                              ##              ##      
3EVB_A         84 GRGGWCYYAAAqk---eVSGVKGFTLGrd----gHEKPMNVQsl--gWNIITFKDKTDihrlepvkcDTLLCDIGESSss 154  Yellow fever ...
5IKM_A         86 GRGGWSYYCAGlk---kVTEVKGYTKGgp----gHEEPIPMAty--gWNLVKLHSGKDvffippekcDTLLCDIGESSpn 156  Dengue virus 1
NP_658908    2581 GAGGFSARMCFek---pVAKILGYTIGkd----kHHTPDLNGkk--vYKKFNWEIGDLfkkr--ircDTFVMDIGESHp- 2648 Tamana bat virus
AKL90417      122 GRGGWEQVIAPnp---sVESIHAITFGsgpgrlgHENFTQKPfa--gMHKVKLTYGDArsma-pqqpDFLLFDGGESHp- 194  Guaico Culex ...
YP_009179388  110 GRGGWEQVIASnp---sITHITSVTLGpgpghegHEAFTKIHfp--gREKIRVLYGDArqfp-iashDTLLFDGGESHs- 182  Wuhan aphid v...
YP_009179401  109 GRGGWEQLYAAsp---dVTRITAVTLGkgpghegHEDFTDNYfpgreKVCLRFADATSypi---tahDTLLFDGGESHp- 181  Shuangao inse...
YP_009179405  122 GAGGWGQLLCGlp---tVTLVESFTLGkgpghegHEQHRMISyegreKHVFHYADIKGlpp---mkcDWLFFDGGEEKk- 194  Wuhan cricket...
APG76081      138 GAGGWDQELARrp---eVVKIRSVTYGatattpgHEVFSTLPfa--gREKIDLRYGDAreea-egdyQWVLFDGGESYp- 210  Changjiang Ji...
AVM87250     2661 GRGGWTQLLMGra---nVSHVLMNSLWip----gHEDCHINSdmlgsHKVTPVKGDYRhvyi-pentDWVLNDAGESE-- 2730 Wenzhou shark...
QBQ65056      127 GRGGWSELVKDlegpggITAVSLWERGke---ewMADPAIRRi---nANVKHLKPWRV---------DTLLFDGGETFkr 191  Yanggou tick ...

Feature 1                                                                                         
3EVB_A        155 ---svTEGERTVRVLDTVEKWLAc----gVDNFCVKVLAPYmpDVLEKLELLQrr-fgGTVIRNPlsRNSTHEMYYVSg- 225  Yellow fever ...
5IKM_A        157 ---ptIEEGRTLRVLKMVEPWLR------GNQFCIKILNPYmpSVVETLEQMQrk-hgGMLVRNPlsRNSTHEMYWVSx- 225  Dengue virus 1
NP_658908    2649 -----NQEIEAIGDLKRLNWFKEnv---dCKNWCIKIMVPTh-EQVLHALPKG-----STLIRNPwsRNSTLEMYCVPgs 2714 Tamana bat virus
AKL90417      195 -----NPNKEAQRFHELLVSVTQkhlhpqLKGFIWKILVPTypPTIKYLTEIQakygmGALYRSAhsRTSNIEMYFISt- 268  Guaico Culex ...
YP_009179388  183 -----DADIEEARFYSLFYNVVMrqinsqTKHFVLKVLTPTspRIQKLLEEIQrmtgrGAFYRSChsRLSTMELYFVSt- 256  Wuhan aphid v...
YP_009179401  182 -----DFSVEANRFNQLFAGAVMrqitpnTKTFILKILTPTspETIRMMQAIQtmtgrGSLYRASvsRNSTLELYFVSt- 255  Shuangao inse...
YP_009179405  195 -----TPEETRDKFNDLLIRGVLphvgfhLKGFIIRVLTPTdtYTLQVLEDIQkltgrGNFVRSTysRNTTQELYFVSg- 268  Wuhan cricket...
APG76081      211 -----DPVREADKFYALLTTVTRkhi-kpTTNFILKILTPTdeRVSKYLKEIQeitgtGALYRSSqsRTTSTELYFISt- 283  Changjiang Ji...
AVM87250     2731 -----GVNDMAKQDVSKLQHYRNt-----PGKKVLKLMVPWheEVQEELKNWTg----YNLYRTDysRNSTSEMYLMP-- 2794 Wenzhou shark...
QBQ65056      192 dqnikKEEDYNDALLDAVDSWMMqp--hpPENFIIKIQVPYtrKALSLMEKWQtrtnkGRLVRLAgeRLSNTVMYFISd- 268  Yanggou tick ...

Feature 1                                  
3EVB_A        226 -ARSNV-----TFTVNQTSRLLMRR 244  Yellow fever virus 17D
5IKM_A        226 -GTGNI-----VSAVNMTSRMLLNR 244  Dengue virus 1
NP_658908    2715 nEPGRM-----VSNVIKVLNQRINR 2734 Tamana bat virus
AKL90417      269 -TPIDI-----SRSIRTIASHIFMK 287  Guaico Culex virus
YP_009179388  257 -GIAPVk----GRAYSLLQSVMIQG 276  Wuhan aphid virus 1
YP_009179401  256 -APTNI-----RNQIRELMQDMARR 274  Shuangao insect virus 7
YP_009179405  269 -QIGALkhqahYLLIDMFKRSLIST 292  Wuhan cricket virus
APG76081      284 -ARTSV-----DTSTRNLLQSVLSR 302  Changjiang Jingmen-like virus
AVM87250     2795 -GTGDK-----TSSAMGCVSTLLNR 2813 Wenzhou shark flavivirus
QBQ65056      269 rLETQL-----RGRVVSFLRELVER 288  Yanggou tick virus

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