2HE2,1PDR,1UM7,5HET,3I4W,5MZ7,1BE9,3JXT


Conserved Protein Domain Family
PDZ3_Dlg1-2-4-like

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cd06795: PDZ3_Dlg1-2-4-like 
PDZ domain 3 of human discs large homolog 1 (Dlg1), Dlg2, and Dlg4, Drosophila disc large (Dlg), and related domains
PDZ (PSD-95 (Postsynaptic density protein 95), Dlg (Discs large protein), and ZO-1 (Zonula occludens-1)) domain 3 of Drosophila Dlg1, human Dlg1, 2, and 4 and related domains. Dlg1 (also known as synapse-associated protein Dlg197; SAP-97), Dlg2 (also known as channel-associated protein of synapse-110; postsynaptic density protein 93, PSD-93), Dlg4 (also known as postsynaptic density protein 95, PSD-95; synapse-associated protein 90, SAP-90) each have 3 PDZ domains and belong to the membrane-associated guanylate kinase family. Dlg1 regulates antigen receptor signaling and cell polarity in lymphocytes, B-cell proliferation and antibody production, and TGFalpha bioavailability; its PDZ3 domain binds pro-TGFalpha, and its PDZ2 domain binds the TACE metalloprotease responsible for cleaving pro-TGFalpha to a soluble form. Dlg2 is involved in N-methyl-D-aspartate (NMDA) receptor signaling, regulating surface expression of NMDA receptors in dorsal horn neurons of the spinal cord; it interacts with NMDA receptor subunits and with Shaker-type K+ channel subunits to cluster into a channel complex. The Dlg4 PDZ1 domain binds NMDA receptors, and its PDZ2 domain binds neuronal nitric oxide synthase (nNOS), forming a complex in neurons. The Drosophila Scribble complex (Scribble, Dlg, and lethal giant larvae) plays a role in apico-basal cell polarity, and in other forms of polarity, including regulation of the actin cytoskeleton, cell signaling and vesicular trafficking, and in tumor development; postsynaptic targeting of Drosophila DLG requires interactions mediated by the first two PDZ domains. PDZ domains usually bind in a sequence-specific manner to short peptide sequences located at the C-terminal end of their partner proteins (known as PDZ binding motifs). The PDZ superfamily includes canonical PDZ domains as well as those with circular permutations and domain swapping mediated by beta-strands. This Dlg-like family PDZ3 domain is a canonical PDZ domain containing six beta-strands A-F and two alpha-helices (alpha-helix 1 and 2), arranged in the order: beta-strands A, B, C, alpha-helix 1, beta-strands D, E, alpha-helix 2 and beta-strand F.
Statistics
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PSSM-Id: 467257
Aligned: 28 rows
Threshold Bit Score: 153.279
Created: 19-Jun-2007
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
 
peptide binding
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:peptide binding site [polypeptide binding site]
Evidence:
  • Comment:based on canonical PDZ domains with structure
  • Comment:PDZ domains specifically recognize and bind to short C-terminal peptide motifs, but can also recognize internal peptide motifs and certain lipids
  • Structure:1BE9: PDZ domain 3 of Rattus norvegicus Dlg4 binds C-terminal peptide derived from a PDZ3-interacting protein identified using a yeast two-hybrid screen; contacts at 4A
  • Citation:PMID 8674113
  • Structure:2HE2: PDZ domain 3 of human Dlg2 binds the self-binding motif (ETSV) extension of another Dlg2 PDZ domain, contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                    #######          #  #                             #   #  ##         
2HE2_A         3 EPRKVVLHKGSTGLGFNIVGGEDGEGIFVSFILAGGPADLSGELQRGDQILSVNGIDLRgASHEQAAAALKGAgQTVTII 82   human
1BE9_A        14 EPRRIVIHRGSTGLGFNIIGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRnASHEQAAIALKNAgQTVTII 93   Norway rat
P31007       503 EPRTITIQKGPQGLGFNIVGGEDGQGIYVSFILAGGPADLGSELKRGDQLLSVNNVNLThATHEEAAQALKTSgGVVTLL 582  fruit fly
ELU16460     246 EPRKIIMKKGTTGLGFNIVGGEDGEGIFVSFILAGGPADLSGVLRRGDQLISVNGIDLRdANHEQAAAALKSSgDTVEIV 325  Capitella teleta
XP_013414731  60 EPRKVTLKKGSSGLGFNIVGGEDGEGIYVSFILAGGPADLSGELKRGDQLISVNGVDLRdATHEQAAAALKHAgDTVEIV 139  Lingula anatina
XP_006825390  29 EPRKVVLQKGTTGLGFNIVGGEDGEGIFISFILAGGVADLSGELKRGDQILSVNSKDLVlATHEEAAAALKNAgQTVTIV 108  Saccoglossus k...
EFX75703     356 TPRSVVLSKGTTGLGFNIVGGEDGEGIFISFILAGGPADVSGQLRRGDQILSVNGHDLKhATHEQAALTLKGAgNTVTLC 435  Daphnia pulex
XP_012556528 320 EPRIVELHRGPSGLGFNIVGGEDGEGIFISFILAGGPADLSGHVKRGDQILMVNDIDIVtATHEKAAAALKGAgLTVNLK 399  Hydra vulgaris
XP_018670811 374 EVRFVTLNKTGVGLGFNIVGGDGSEGIFISYILAGGTADVSGELFRGDQLLSVNGIDLTkATHEEAAHALKSAdKVVTIG 453  vase tunicate
NP_001024431 507 EPRPVQLVKGQNGLGFNIVGGEDNEPIYISFVLPGGVADLSGNVKTGDVLLEVNGVVLRnATHKEAAEALRNAgNPVYLT 586  nematode
Feature 1                   
2HE2_A        83 AQyqPEDYARF 93   human
1BE9_A        94 AQykPEEYSRF 104  Norway rat
P31007       583 AQyrPEEYNRF 593  fruit fly
ELU16460     326 AQyrPEDYNRF 336  Capitella teleta
XP_013414731 140 AQykPEDYNRF 150  Lingula anatina
XP_006825390 109 AQykPEEYNRF 119  Saccoglossus kowalevskii
EFX75703     436 VQyrPEEYNRF 446  Daphnia pulex
XP_012556528 400 LQykPEEYNRF 410  Hydra vulgaris
XP_018670811 454 AQykPEDYNRF 464  vase tunicate
NP_001024431 587 LQyrPQEYQIF 597  nematode

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