GEO DataSets

(Submitter supplied) An essential interaction between sunlight and eukaryotes involves the production of vitamin D through exposure to ultraviolet (UV) radiation. While extensively studied in vertebrates, the role of vitamin D in non-animal eukaryotes like microalgae remains unclear. To investigate the potential involvement of vitamin D in the response of microalgae to UV, we focus on Emiliania huxleyi, a microalga found in shallow ocean depths that are exposed to UV radiation. more...

Organism:

Emiliania huxleyi

Type:

Expression profiling by high throughput sequencing

Platform:

GPL33777

18 Samples

Download data: TXT

(Submitter supplied) purose:The large-scale reproduction of Phaeocystis globosa has caused serious damage to the marine ecosystem in the coastal waters of China. The outbreak of algae blooms depends on the competitive advantage of their heteromorphic life history: colonial formation is beneficial to resist zooplankton predation, and the single isolated cells can absorb nutrients rapidly. To better understand the different types of cells characteristics of P. more...

Organism:

Phaeocystis globosa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27813

6 Samples

Download data: FASTA, TXT

(Submitter supplied) In order to determine how the bacterial quorum-sensing precursor molecule 2-heptyl-4-quinolone (HHQ) induces cellular senescence in populations of some eukaryotic marine phytoplankton, we examined the transcriptional response of Emiliania huxleyi CCMP2090 to HHQ exposure. Quadruplicate batch cultures were treated with HHQ at three concentrations (1, 10 and 100 ng/ml) and compared to a vehicle control after 24 and 72 hours of exposure. more...

Organism:

Emiliania huxleyi CCMP1516

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26711

36 Samples

Download data: TXT

(Submitter supplied) We performed single-cell transcriptome analysis (using MARS-seq) for the worldwide-distributed microalga Emiliania huxleyi during infection by its specific coccolithovirus. Our results provide insights into the expression programs and infection strategies of the large virus, and highlight the potential of single-cell RNA-sequencing for microbial eukaryotes in the lab and in the field.

Organism:

Coccolithovirus; Emiliania huxleyi

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL27010 GPL27011

8 Samples

Download data: TXT

(Submitter supplied) purpose:The large-scale reproduction of Phaeocystis globosa has caused serious damage to the marine ecosystem in the coastal waters of China. The outbreak of algae blooms depends on the competitive advantage of their heteromorphic life history: colonial formation is beneficial to resist zooplankton predation, and the single isolated cells can absorb nutrients rapidly. methods:For RNA exaction, the biomass was resuspended in 2 mL RNA extraction buffer (1:1 mix of aqua-phenol and buffer L [0.5% SDS, 10 mM EDTA, 0.2 M sodium acetate (pH 5), and 1:100 β-mercaptoethanol)] and then incubated with DNase I (Takara, Japan) for 30 min at 37 °C to remove genomic DNA. more...

Organism:

Phaeocystis globosa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL27813

6 Samples

Download data: TXT

(Submitter supplied) Dnmt1 epigenetically propagates symmetrical CG methylation in many eukaryotes. Their genomes are typically depleted of CG dinucleotides because of imperfect repair of deaminated methylcytosines. Here, we extensively survey diverse species lacking Dnmt1 and show that, surprisingly, symmetrical CG methylation is nonetheless frequently present and catalyzed by a different DNA methyltransferase family, Dnmt5. more...

Organism:

Phaeodactylum tricornutum; Emiliania huxleyi; Micromonas pusilla; Aureococcus anophagefferens; Ostreococcus sp. 'lucimarinus'; Bathycoccus prasinos; Cryptococcus neoformans var. grubii; Fragilariopsis cylindrus; Leishmania major; Thalassiosira pseudonana; Cyanidioschyzon merolae

Type:

Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

12 related Platforms

26 Samples

Download data: GFF, GTF