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High-throughput pyrosequencing of plant virus small RNAs

(Submitter supplied) Plant virus infection involves the production of viral small RNAs (vsRNAs) with the potential to associate with distinct Argonaute (AGO)-containing silencing complexes and mediate diverse silencing effects on RNA and chromatin. We used multiplexed, high-throughput pyrosequencing to profile populations of vsRNAs from plants infected with viruses from different genera. Sense and antisense vsRNAs of 20 to 24 nucleotides (nts) spread throughout the entire viral genomes in an overlapping configuration; virtually all genomic nucleotide positions were represented in the dataset. more...
Organism:
Melon necrotic spot virus; Potato virus X; Turnip mosaic virus; Cucumber mosaic virus; Tomato yellow leaf curl virus; Cymbidium ringspot virus; Pepper mild mottle virus; Tobacco rattle virus; Watermelon mosaic virus
Type:
Non-coding RNA profiling by high throughput sequencing
9 related Platforms
10 Samples
Download data: TXT
Series
Accession:
GSE16996
ID:
200016996

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