GEO DataSets

(Submitter supplied) microRNAs are ∼22 nucleotide regulatory RNAs that are processed into duplexes from hairpin structures and incorporated into Argonaute proteins. We find that a nick in the middle of the guide strand of an miRNA sequence allows for seed-based targeting characteristic of miRNA activity. Insertion of an inverted abasic, a dye, or a small gap between the two segments still permits target knockdown. While activity from the seed region of the segmented miRNA is apparent, activity from the 3' half of the guide strand is impaired, suggesting that an intact guide backbone is required for contribution from the 3' half. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3774

Platform:

GPL10379

4 Samples

Download data: CEL

Analysis of HCT-116 dicer- cells transfected with miR-124 duplex containing a segmented guide strand. Following miRNA guide strand segmentation, seed-based (positions 2 through 8 at the 5' end of the guide strand) activity persisted. Results illustrate a structural flexibility in miRNA duplexes.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 protocol sets

Platform:

GPL10379

Series:

GSE25224

4 Samples

Download data: CEL

(Submitter supplied) Contains probesets for each transcript. Probeset is made of 4 to 20 distinct probes. Protocol: See manufacturer website.

Organism:

Homo sapiens

2 DataSets

19 Series

2879 Samples

Download data: CDF, TXT