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Items: 6

1.

Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP

(Submitter supplied) While NGS allows rapid global detection of transcripts, it remains difficult to distinguish ncRNAs from short mRNAs. To detect potentially translated RNAs, we developed an improved protocol for bacterial ribosomal footprinting (RIBOseq). This allowed distinguishing ncRNA from mRNA in EHEC. A high ratio of ribosomal footprints per transcript (ribosomal coverage value, RCV) is expected to indicate a translated RNA, while a low RCV should point to a non-translated RNA. more...
Organism:
Escherichia coli O157:H7 str. EDL933
Type:
Expression profiling by high throughput sequencing; Other
Platform:
GPL23073
4 Samples
Download data: XLSX
Series
Accession:
GSE94984
ID:
200094984
2.

Illumina MiSeq (Escherichia coli O157:H7 str. EDL933)

Organism:
Escherichia coli O157:H7 str. EDL933
2 Series
10 Samples
Download data
Platform
Accession:
GPL23073
ID:
100023073
3.

EHEC in LB Experiment 2 [RIBO-Seq]

Organism:
Escherichia coli O157:H7 str. EDL933
Source name:
biomass collected in the transition between exponential to stationary phase
Platform:
GPL23073
Series:
GSE94984
Download data
Sample
Accession:
GSM2493798
ID:
302493798
4.

EHEC in LB Experiment 2 [RNA-Seq]

Organism:
Escherichia coli O157:H7 str. EDL933
Source name:
biomass collected in the transition between exponential to stationary phase
Platform:
GPL23073
Series:
GSE94984
Download data
Sample
Accession:
GSM2493797
ID:
302493797
5.

EHEC in LB Experiment 1 [RIBO-Seq]

Organism:
Escherichia coli O157:H7 str. EDL933
Source name:
biomass collected in the transition between exponential to stationary phase
Platform:
GPL23073
Series:
GSE94984
Download data
Sample
Accession:
GSM2493796
ID:
302493796
6.

EHEC in LB Experiment 1 [RNA-Seq]

Organism:
Escherichia coli O157:H7 str. EDL933
Source name:
biomass collected in the transition between exponential to stationary phase
Platform:
GPL23073
Series:
GSE94984
Download data
Sample
Accession:
GSM2493795
ID:
302493795
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Supplemental Content

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