GEO DataSets

(Submitter supplied) PC12 cells (passage 22) were cultured at 5% CO2 in a 37 C incubator. The growth medium was DMEM (high glucose, Invitrogen) supplemented with 25mM HEPES, 10% FCS, 5% FBS and 1x Pen/Strep (Invitrogen). 1x106 cells were starved overnight in DMEM+25 mM HEPES and treated with 10 uM 1,9 dideoxy forskolin or forskolin for 60 min. The final DMSO concentration was 0.05%. Total RNA was purified using Trizol (Invitrogen) according to the manufacturers protocol. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Datasets:

GDS1038 GDS1039

Platforms:

GPL342 GPL341

8 Samples

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Expression profiling of PC12 pheochromocytoma cells treated with 10 uM forskolin for 1 hour. Forskolin activates adenylate cyclase. Effect of 1,9 dideoxy forskolin, an inactive analog of forskolin, also examined. Results identify genes whose expression responds to changes in cAMP levels.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL342

Series:

GSE2071

4 Samples

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(Submitter supplied) Affymetrix submissions are typically submitted to GEO using the GEOarchive method described at http://www.ncbi.nlm.nih.gov/projects/geo/info/geo_affy.html Array B of GeneChip Rat Expression Set 230 Has 15333 entries and was indexed 09-Apr-2003 Sequences used in the design of the array were selected from GenBank, dbEST, and RefSeq. Sequence clusters were created from Build 99 of UniGene (June 2002) and refined by analysis and comparison with a number of other publicly available databases including the Baylor College of Medicine Human Genome Sequencing Center's preliminary rat genome assembly (June 2002). more...

Organism:

Rattus norvegicus

8 DataSets

16 Series

179 Samples

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