GEO DataSets

(Submitter supplied) Cytosine deaminases have important uses in the detection of epigenetic modifications and in genome editing. However, the range of applications of deaminases is limited by their substrate preference. To expand the toolkit of deaminases, we developed an in-vitro approach that bypasses a major hurdle with their severe toxicity in expression hosts. We screened 175 putative cytosine deaminases, primarily from bacteria, and found enzymes with strong activity on double- and single-stranded DNA in various sequence contexts, including some without any sequence constraints. more...

Organism:

Homo sapiens; Escherichia coli

Type:

Other

Platforms:

GPL14548 GPL32081 GPL24676

478 Samples

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