GEO DataSets

(Submitter supplied) Ammonia-oxidizing archaea (AOA) have been reported at high abundance in much of the global ocean, even in environments such as pelagic oxygen minimum zones (OMZs), where conditions seem unlikely to support aerobic ammonium oxidation. Due to the lack of information on any potential alternative metabolism of AOA, the AOA community composition might be expected to differ between oxic and anoxic environments, indicating some difference in ecology and/or physiology of the AOA assemblage. more...

Organism:

uncultured crenarchaeote; marine metagenome; synthetic construct

Type:

Other

Platform:

GPL17151

23 Samples

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(Submitter supplied) Microarray targets the ammonia monooxygenase gene subunit A (amoA). Protocol: 1.The probe oligonucleotides are printed onto Corning Ultragaps or Erie Scientific Epoxy Silane slides using a DeRisi style arrayer (DiRisi et al., 1997) and pins from Parallel Synthesis. 2.The 70-mer oligo probes (Operon Technologies) were adjusted to a concentration of 0.05 mg ml-1 in 50% DMSO and were spotted on CMT-GAPS amino silane-coated glass slides (Corning, Corning, NY). more...

Organism:

uncultured crenarchaeote

1 Series

23 Samples

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(Submitter supplied) The array contains three sets of probes for different applications (rbcL and nitrite reductase (NR) from phytoplankton and amoA from ammonia-oxidizing archaea). The paper to which this submission relates used on the amoA probe set. See linked sequence list for additional details. Protocol: 90-mer oligonucleotides, spotted using DiRisi type arrayer

Organism:

uncultured crenarchaeote

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