GEO DataSets

(Submitter supplied) In vitro gametogenesis, the process of generating gametes from pluripotent cells in culture, is a powerful tool for improving our understanding of germ cell development as well as an alternative source of gametes.(1) Conservation of the northern white rhinoceros (NWR), a species for which only two females remain, would be a compelling application of in vitro gametogenesis as a gametes source. Here, we established a culture system that induces a robust number of primordial germ cell-like cells (PGCLCs) from pluripotent stem cells of the NWR and southern white rhinoceros (SWR), the closest species to the NWR. more...

Organism:

Ceratotherium simum cottoni; Ceratotherium simum simum

Type:

Expression profiling by high throughput sequencing

Platforms:

GPL31181 GPL31180

26 Samples

Download data: TXT

(Submitter supplied) The northern white rhinoceros (NWR) is probably the earth’s most endangered mammal. To rescue the functionally extinct species, we aim to employ induced pluripotent stem cells (iPSCs) to generate gametes and subsequently embryos in vitro. To elucidate the regulation of pluripotency and differentiation of NWR PSCs, we generated iPSCs from a deceased NWR female using episomal reprogramming, and observed surprising similarities to human PSCs. more...

Organism:

Ceratotherium simum cottoni

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29391

9 Samples

Download data: GTF, TSV