GEO DataSets

(Submitter supplied) 5-methyl-cytosine DNA methylation regulates gene expression and developmental programming in a broad range of eukaryotes. However, its presence and potential roles in ciliates, complex single-celled eukaryotes with germline-somatic genome specialization via nuclear dimorphism, are largely uncharted. While canonical cytosine methyltransferases have not been discovered in published ciliate genomes, recent studies performed in the stichotrichous ciliate Oxytricha trifallax suggest de novo cytosine methylation during macronuclear development. more...

Organism:

Paramecium tetraurelia

Type:

Methylation profiling by high throughput sequencing

Platform:

GPL24715

3 Samples

Download data: TXT

(Submitter supplied) At each sexual cycle, during development of the somatic macronucleus (MAC) from the germline micronucleus (MIC), the genome of the ciliate Paramecium tetraurelia is massively rearranged through the reproducible elimination of germline-specific sequences. It has been reported previously that targeting of these sequences is mediated by non-protein-coding RNAs including different classes of small RNAs and longer non-coding transcripts. more...

Organism:

Paramecium tetraurelia

Type:

Expression profiling by array

Platform:

GPL19603

12 Samples

Download data: CALLS, PAIR

(Submitter supplied) Differential transcriptome of Paramecium tetraurelia strain 51 undergoing RNAi by feeding against ICL7a (as a control) and RDR3 for nine days.

Organism:

Paramecium tetraurelia

Type:

Expression profiling by array

Platform:

GPL18944

6 Samples

Download data: PAIR

(Submitter supplied) We used microarrays to find differentially expressed genes between reactive cells of mating type 7 and 8

Organism:

Paramecium tetraurelia

Type:

Expression profiling by genome tiling array

Platform:

GPL7221

2 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Paramecium tetraurelia

Type:

Expression profiling by array; Expression profiling by genome tiling array

Platform:

GPL7221

58 Samples

Download data: PAIR, TAB, TXT

(Submitter supplied) P. tetraurelia, like all ciliates, is a unicellular eukaryote processing two different kinds of nuclei, germline micronuclei (mic) and somatic macronucleus (mac). The diploid micronuclei undergo meiosis during sexual events to transmit the germline genome to the next generation. The highly polyploid mac (~800 n) is responsible for transcription during the life cycle but is lost after fertilisation; the new mic and mac develop from mitotic copies of the zygotic nucleus. more...

Organism:

Paramecium tetraurelia

Type:

Expression profiling by genome tiling array

Platform:

GPL7221

11 Samples

Download data: TXT

(Submitter supplied) In the ciliate Paramecium tetraurelia, autogamy is a self-fertilization process, during which the zygotic nucleus results from the fusion of two identical gametic nuclei. This phenomenon occurs in response to starvation. It starts with meiosis of the germline nuclei (micronuclei or MIC) and fragmentation of the parental somatic nucleus (macronucleus or MAC). This is followed by mitotic division of one haploid nucleus issued from meiosis to yield two identical gametic nuclei, then karyogamy takes place, followed by mitosis of zygotic nucleus and differentiation of new MICs and MACs from the resulting copies of the zygotic nucleus. more...

Organism:

Paramecium tetraurelia

Type:

Expression profiling by genome tiling array

Platform:

GPL7221

6 Samples

Download data: TXT

(Submitter supplied) In the ciliate Paramecium tetraurelia, autogamy is a self-fertilization process, during which the zygotic nucleus results from the fusion of two identical gametic nuclei. This phenomenon occurs in response to starvation. It starts with meiosis of the germline nuclei (micronuclei or MIC) and fragmentation of the parental somatic nucleus (macronucleus or MAC). This is followed by mitotic division of one haploid nucleus issued from meiosis to yield two identical gametic nuclei, then karyogamy takes place, followed by mitosis of zygotic nucleus and differentiation of new MICs and MACs from the resulting copies of the zygotic nucleus. more...

Organism:

Paramecium tetraurelia

Type:

Expression profiling by genome tiling array

Platform:

GPL7221

4 Samples

Download data: TXT

(Submitter supplied) In the ciliate Paramecium tetraurelia, autogamy is a self-fertilization process, during which the zygotic nucleus results from the fusion of two identical gametic nuclei. This phenomenon occurs in response to starvation. It starts with meiosis of the germline nuclei (micronuclei or MIC) and fragmentation of the parental somatic nucleus (macronucleus or MAC). This is followed by mitotic division of one haploid nucleus issued from meiosis to yield two identical gametic nuclei, then karyogamy takes place, followed by mitosis of zygotic nucleus and differentiation of new MICs and MACs from the resulting copies of the zygotic nucleus. more...

Organism:

Paramecium tetraurelia

Type:

Expression profiling by genome tiling array

Platform:

GPL7221

12 Samples

Download data: TXT

(Submitter supplied) Paramecium cells possess a few thousand trichocysts (big secretory granules of 3~4 um long) docked at their surface and whose discharge in the external medium can be triggered using aminoethyldextran (a modified dextran positively charged) without damage to the cells. After discharge, new trichocysts are re-synthesized by the cells. We studied the evolution of the transcriptome during this synthesis.

Organism:

Paramecium tetraurelia

Type:

Expression profiling by genome tiling array

Platform:

GPL7221

12 Samples

Download data: PAIR, TXT

(Submitter supplied) Total RNA was extracted at different stages (TV, T0, T11 and T20) during autogamy of strain d4-2. Genes specifically induced during autogamy were identified following analysis of microarray hybridization data

Organism:

Paramecium tetraurelia

Type:

Expression profiling by array

Platform:

GPL7296

12 Samples

Download data: GPR, XLS

(Submitter supplied) Paramecium cells in stationary phase were treated for deciliation and total mRNA extracted at two time points (45 and 130 minutes) after deciliation. Keywords: Time course analysis of expression during reciliation

Organism:

Paramecium tetraurelia

Type:

Expression profiling by array

Platform:

GPL7221

5 Samples

Download data: PAIR, TAB

(Submitter supplied) Paramecium cells in log-phase growth were treated for deciliation and total mRNA extracted at two time points after deciliation. This is usefull to study genes involved in cilia biogenesis. Time points are : T0 : mRNA extracted before deciliation. T30 : mRNA extracted 30 minutes after deciliation. T120 : mRNA extracted 120 minutes after deciliation.

Organism:

Paramecium tetraurelia

Type:

Expression profiling by array

Platform:

GPL7221

8 Samples

Download data: PAIR, TAB

(Submitter supplied) In situ oligonucleotide synthesis as described in www.nimblegen.com

Organism:

Paramecium tetraurelia

1 Series

12 Samples

Download data: NDF

(Submitter supplied) NimbleGen-P.tetraurlia-SET01-02-50mer SET01 contains three sense and three as-probes per annotated transcript (previoulsy described in Arnaiz et al. BMC Gemomics 2010); SET02 was redesigned by Nimblegen and contains 12 as-probes per annotated transcript Protocol: In situ oligonucleotide synthesis as described in www.nimblegen.com

Organism:

Paramecium tetraurelia

1 Series

6 Samples

Download data: NCD, NDF, TXT

(Submitter supplied) GapsII aminosilane coated slides were used Protocol: - Array were spotted with the GeneTAC G3 (Genomic solutions) - cDNA were dried and resuspended in 50% formamide and 1% CHAPs (spotting buffer) - Each array were qualified after spotting by scan with the axon 4000B - Each array were designed to contain 3 replicates of each probe

Organism:

Paramecium tetraurelia

1 Series

12 Samples

Download data

(Submitter supplied) The grid of features is 768x1024 (X, Y). It consists of 11 different SETs as described below. SET01 : Probes that match annotated transcripts. Each transcript has 6 probes (3 on the forward strand and 3 on the reverse strand). SET02 : Probes for complete tilling of 4 scaffolds : SCAFFOLD_106, SCAFFOLD_142, SCAFFOLD_164, SCAFFOLD_96. The probes cover both strands of the 4 scaffolds with a 25 nucleotides overlap. more...

Organism:

Paramecium tetraurelia

9 Series

60 Samples

Download data: NDF