GEO DataSets

(Submitter supplied) In this study, we achieved translocated chromosomal duplication and triplication of a 1.4-Mb targeted chromosomal region by directly introducing I-SceI meganuclease into A. oryzae protoplast cells. Strains with duplication and triplication of chromosome 2 showed substantial increases in the activity of protease and amylase. Gene dosage effects were enhanced by combining the structural gene and its regulatory gene, indicating that segmental duplications of chromosomes play important phenotypic roles in koji mold strains.

Organism:

Aspergillus oryzae RIB40; Aspergillus oryzae

Type:

Genome variation profiling by array

Platform:

GPL16017

2 Samples

Download data: TXT

(Submitter supplied) We used microarrays to find out carbon souce responsive genes of A. oryzae with 1hr induction with galactose, glucose, and sorbitol.

Organism:

Aspergillus oryzae RIB40

Type:

Expression profiling by array

Platform:

GPL16184

6 Samples

Download data: CEL, CHP

(Submitter supplied) We here describe the first successful construction of a targeted tandem duplication of a large chromosomal segment in Aspergillus oryzae. The targeted tandem chromosomal duplication was achieved by using strains that had 5’ΔpyrG upstream of the region targeted for tandem chromosomal duplication and 3’ΔpyrG downstream of the target region. Consequently, strains bearing a 210-kb targeted tandem chromosomal duplication near the centromeric region of chromosome 8 and strains bearing a targeted tandem chromosomal duplication of a 700-kb region of chromosome 2 were successfully constructed. more...

Organism:

Aspergillus oryzae RIB40

Type:

Genome variation profiling by array

Platform:

GPL16017

2 Samples

Download data: TXT

(Submitter supplied) In this study, we focused on chemically defined inducers or substrates to drive expression of cellulases, hemicellulases and accessory enzymes in the model filamentous fungus Aspergillus oryzae. Cellohexaose (O-CHE), mannohexaose (O-MHE), xylopentaose (O-XPE), arabinoheptaose (O-AHP), 1,3:1,4-β-glucohexaose (O-BGHEXA), 63-α-D-glucosyl-maltotriosyl-maltotriose (O-GMH), 61-α-D-galactosyl-mannotriose (O-GM3), xyloglucan (X3Glc4-borohydride reduced; O-X3G4R), turanose (TYR) and sophorose (SOP) were used to induce the plant polysaccharide degradation machinery of A. more...

Organism:

Aspergillus oryzae RIB40

Type:

Expression profiling by array

Platform:

GPL17444

32 Samples

Download data: CSV, TXT

(Submitter supplied) Fungal endo-β-mannanases (β-mannanases) are widely used as industrial enzymes; however, no transcriptional regulator of β-mannanases has been identified in fungi or other eukaryotic cells to date. To identify a transcriptional regulator of β-mannanases in Aspergillus oryzae, a gene-disruptant library of transcriptional regulators was screened for mutants exhibiting reduced β-mannanase activity by using konjac glucomannan as the substrate, and ManR, a Zn(II)2Cys6 type DNA binding protein was identified. more...

Organism:

Aspergillus oryzae RIB40

Type:

Expression profiling by array

Platform:

GPL16017

7 Samples

Download data: TXT

(Submitter supplied) In eukaryotic cells , histone deactylase plays key roles in the diverse process such as genomic stabillity, differenciation, and metabolism. These enzymes also conserved in filamentous fungi, and their functios has been started to understand some fungal biological process such as development, stress responce, and secondary metabolism. We found deletion of HstD homolog of yeast Hst4, affected development, secondary metabolism in aspergillyus oryzae. more...

Organism:

Aspergillus oryzae RIB40; Aspergillus oryzae

Type:

Expression profiling by array

Platform:

GPL16184

4 Samples

Download data: CEL, CHP

(Submitter supplied) The potential for sexual reproduction in Aspergillus oryzae was assessed by investigating the presence and functionality of MAT genes. Previous genome studies had identified a MAT1-1 gene in the RIB40 reference strain. We now report the existence of a complimentary MAT1-2 gene and sequencing of an idiomorph region from A. oryzae strain AO6. This allowed the development of a PCR diagnostic assay, which detected isolates of MAT1-1 and MAT1-2 genotype among 180 strains assayed including industrial tane-koji isolates. more...

Organism:

Aspergillus oryzae RIB40; Aspergillus oryzae

Type:

Expression profiling by array

Platform:

GPL13996

2 Samples

Download data: CEL, CHP

(Submitter supplied) Hypoxia imposes stress on filamentous fungi that require oxygen to proliferate. Global transcription analysis of Aspergillus oryzae grown under hypoxic conditions found that the expression of about 50% of 4,244 affected genes was either induced or repressed more than 2-fold. A comparison of these genes with the hypoxically-regulated genes of A. nidulans (Masuo et al., Mol. Gen. Genet. 2010, 284:415-424) based on their predicted amino acid sequences classified them as bi-directional best hit (BBH), one-way best hit (extra homolog: EH) and no-hit (non-syntenic genes: NSG) genes. more...

Organism:

Aspergillus oryzae RIB40

Type:

Expression profiling by array

Platform:

GPL13996

7 Samples

Download data: CEL, CHP

(Submitter supplied) see manufacturer's web site

Organism:

Aspergillus oryzae RIB40

1 Series

32 Samples

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(Submitter supplied) see manufacturer's web site

Organism:

Aspergillus oryzae RIB40

3 Series

11 Samples

Download data

(Submitter supplied) see manufacturer's web site (http://www.affymetrix.com/support/technical/manuals.affx)

Organism:

Aspergillus oryzae RIB40

2 Series

10 Samples

Download data: CDF, CIF, GIN, GRC, PSI, SIF, TXT

(Submitter supplied) see manufacturer's web site

Organism:

Aspergillus oryzae RIB40

2 Series

9 Samples

Download data: CDF, CIF, GIN, GRC, PSI, SIF, TXT

(Submitter supplied) At least one oligo was designed from each gene model larger then 100 amino acids. For genes with more than one exon, oligos were designed based on the second exon sequence. For genes bigger than 4 kb, two oligos were designed from 5’ and 3’ ends. Protocol: The 70-mer oligo microarray was designed based on CDS annotated from the A. flavus NRRL 3357 whole genome sequence. A total of 12,160 oligos were synthesized by Invitrogen. more...

Organism:

Aspergillus flavus NRRL3357; Aspergillus oryzae RIB40

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