GEO DataSets

(Submitter supplied) Most porcine embryonic stem cells (pESC) derived from in vitro blastocysts Still require complex media compositions and feeder layers which make difficulty to adapt their broad and routine use. We derived pESC using simplified culture media (FIW) consisting of small molecules FGF2, IWR-1, and WH-4-023 in serum-free medium. We established several pESC-FIW lines capable of single cell passaging with short cell doubling time (about 12 hours). more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

6 Samples

Download data: XLSX

(Submitter supplied) Influenza A virus (IAV) infection is a global respiratory disease, which annually leads to 3-5 million cases of severe illness, resulting in 290,000-650,000 deaths. Additionally, during the past century, four global IAV pandemics have claimed millions of human lives. The epithelial lining of the trachea plays a vital role during IAV infection, both as point of viral entry and replication as well as in the antiviral immune response. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL29847

22 Samples

Download data: XLSX

(Submitter supplied) Female and male pigs were studied. Half of the pigs were exposed to immune activation from virally-infected mothers, and half were not exposed. At 60 days of age about one third of the pigs were given metabolic distress (fasting), one third of the pigs were given immune distress (poly(I:C)), and one third of the pigs were given Saline (control).

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL22475

112 Samples

Download data: CSV

(Submitter supplied) Biobanking of tissue from clinically obtained kidney biopsies for later analysis with multi-omic approaches, such as single cell technologies, proteomics, metabolomics and the different types of imaging, is an inevitable step to overcome the need of disease model systems and towards translational medicine. Hence, collection protocols that ensure integration into daily clinical routines by the usage of preservation media that do not require liquid nitrogen but instantly preserve kidney tissue for both clinical and scientific analyses are necessary. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

6 Samples

Download data: H5

(Submitter supplied) Long noncoding RNAs (lncRNAs) participate in regulating many biological processes. However, their roles in African swine fever virus(ASFV) pathogenicity are largely unknown. Here, we analyzed the expression profile of lncRNAs and mRNAs in the ASFV-infected or uninfected PAMs by high-throughput sequencing

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing

Platform:

GPL26351

6 Samples

Download data: FA, GTF, XLS

(Submitter supplied) Studies on porcine induced pluripotent stem (iPS) cells served as a great reference for human clinical research due to the similarity in organ size, physiology, anatomy, and heredity between pig and human. However, the reprogramming efficiency of iPS cells was currently poor. In this study, we reported the induction of iPS cells from porcine Sertoli cells (SCs) via infecting with four separate lentiviral vectors expressing pig OCT4, SOX2, KLF4 and c-MYC. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26285

6 Samples

Download data: TXT

(Submitter supplied) Develop methods to elucidate the pig antibody repertoire using single B cell sequencing

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL34588

2 Samples

Download data: CSV, H5

(Submitter supplied) Oocyte maturation refers to oocytes at the germinal vesicle stage progressing into metaphase II (MII) stage of development. Even though numerous studies have shown key genes and potential important signalling cascades, which drive the GV to MII transition, a system-wide analysis of underlying differences at gene level and especially at transcript level between the two developmental stages of the oocyte is still lacking. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL20983

15 Samples

Download data: CSV, TSV

(Submitter supplied) Chloroquine (CQ) could function as a lysosomotropic inhibitor to impair the autophagy-lysosome pathway, and is widely used to treat malarial, tumor and recently COVID-19. However, the effect of CQ treatment on porcine immature Sertoli cells (iSCs) remains unclear.;Here we showed that CQ treatment reduced iSCs viability in a dose-dependent manner. CQ treatment (20µM) of iSCs for 36h could elevate oxidative stress, damage mitochondrial function and promote apoptosis. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

9 Samples

Download data: TXT

(Submitter supplied) Damage to the development of porcine gametes and embryos caused by high temperatures (HT) is one of the main reasons for the decline in the economic benefits of the livestock industry. Zygotic genome activation (ZGA) marks the beginning of gene expression programs in mammalian pre-implantation embryos. In pigs, ZGA occurs at the 4-cell (4C) stage, indicating that correct gene expression at this stage plays an important regulatory role in embryonic development. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

6 Samples

Download data: TXT

(Submitter supplied) Supplementing diets with olive by-products offers promising benefits for alleviating animal feeding stress without compromising growth. The aim of this study was to explore the transcriptome of backfat in Iberian pigs fed with two diets based in olive by-products. The pigs were divided in three diet groups: a Control (C) group, a dry olive pulp (DOP) group, which was fed a compound feed elaborated with olive pulp, and a wet crude olive cake (WCOC) group, which was fed a compound feed and olive cake silage supplied ad libitum. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL11429

15 Samples

Download data: TSV

(Submitter supplied) PRV infection in swine can cause devastating disease and pose a potential threat to humans. Advancing the interplay between PRV and host is essential to elucidate the pathogenic mechanism of PRV and identify novel anti-PRV targets. In this study, we identified PARP11 as a host factor that can significantly affect PRV infection. Inhibition of PARP11 and knockout of PARP11 can significantly promoted PRV infection. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

6 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26351

52 Samples

Download data: BROADPEAK, BW, NARROWPEAK

(Submitter supplied) These data are transcriptome sequencing data of porcine muscle satellite cells at the stage of proliferation and differentiation.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

12 Samples

Download data: CSV

(Submitter supplied) The data are the cut&tag data sequencing data of H3K4me3, H3K27Ac and H3K27me3 of pig muscle satellite cells at the stage of proliferation and differentiation.

Organism:

Sus scrofa

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26351

32 Samples

Download data: BROADPEAK, BW, NARROWPEAK

(Submitter supplied) These data are ATAC-Seq data of porcine muscle satellite cells at the stage of proliferation and differentiation.

Organism:

Sus scrofa

Type:

Genome binding/occupancy profiling by high throughput sequencing

Platform:

GPL26351

8 Samples

Download data: BW, NARROWPEAK

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing; Other

Platform:

GPL26351

2 Samples

Download data: MTX, TIFF, TSV

(Submitter supplied) Mesenchymal environments are considered to maintain the quiescent state of SDL within the stem-cell niche. We used single cell RNA sequencing (scRNA-seq) to analyze the subtypes and functions of the mesenchyme surrounding SDL.

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

1 Sample

Download data: MTX, TSV

(Submitter supplied) Mesenchymal environments are considered to maintain the quiescent state of SDL within the stem-cell niche. To reveal expressional heterogeneity and cell-cell interaction in the mesenchyme and the dental epithelium, we performed spatial transcriptome to analyze the subtypes and functions of the mesenchyme surrounding SDL.

Organism:

Sus scrofa

Type:

Other

Platform:

GPL26351

1 Sample

Download data: MTX, TIFF, TSV

(Submitter supplied) Early-life sepsis remains one of the leading causes of global morbidity and mortality in the neonatal population, especially preterm infants. Using a preterm piglet model of neonatal sepsis to test nutritional interventions as a therapeutic approach, we demonstrated that high parenteral glucose induced hyperglycemia with severe sepsis, whereas glucose restriction offered protection against infection but led to severe hypoglycemia. more...

Organism:

Sus scrofa

Type:

Expression profiling by high throughput sequencing

Platform:

GPL26351

74 Samples

Download data: TXT