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MP3-seq: Massively parallel measurement of protein-protein interactions by sequencing

(Submitter supplied) Abstract: Protein-protein interactions (PPIs) regulate many cellular processes, and engineered PPIs have cell and gene therapy applications. Here we introduce massively parallel protein-protein interaction measurement by sequencing (MP3-seq), an easy-to-use and highly scalable yeast-two-hybrid approach for measuring PPIs. In MP3-seq, DNA barcodes are associated with specific protein pairs, and barcode enrichment can be read by sequencing to provide a direct measure of interaction strength. more...
Organism:
Saccharomyces cerevisiae
Type:
Other
Platforms:
GPL26302 GPL17143
36 Samples
Download data: CSV, XLSX
Series
Accession:
GSE271790
ID:
200271790

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