GEO DataSets

Analysis of MyD88 null mutant macrophages treated with LPS or live E. coli. MyD88 transduces cell signaling events downstream of Toll-like receptors, a key component of host defense. Results suggest most of the host response to endotoxin or live bacteria is actually regulated independently of MyD88.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 agent, 2 genotype/variation sets

Platform:

GPL1226

Series:

GSE1405

16 Samples

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(Submitter supplied) Comparison of gene expression in wildtype and MyD88-/- C57BL/6J mouse macrophages treated with 10 ng/mL LPS for 2 hours versus media treated control macrophages, and, wildtype and MyD88-/- C57BL/6J mouse macrophages treated with live E. coli bacteria (log phase; 1 bact per 1 macrophage) for 2 hours versus media treated control macrophages. Cells from 4 mice of each geneotype were used and each individual provided its own control. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS1705 GDS1706

Platform:

GPL1226

31 Samples

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Analysis of MyD88 null mutant macrophages treated with LPS or live E. coli. MyD88 transduces cell signaling events downstream of Toll-like receptors, a key component of host defense. Results suggest most of the host response to endotoxin or live bacteria is actually regulated independently of MyD88.

Organism:

Mus musculus

Type:

Expression profiling by array, log2 ratio, 2 agent, 2 genotype/variation sets

Platform:

GPL1226

Series:

GSE1405

15 Samples

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(Submitter supplied) This series represents a comparison of gene expression in mouse aorta from MyD88-/-, apoE-/- mice versus apoE-/- control mice fed a high fat diet for 8 weeks, causing atherosclerotic lesion development. 5 MyD88-/-, apoE-/- mice were compared to 5 apoE-/- control mice and dye swap replicated for a total of 10 replicate microarrays. Keywords = Mus musculus Keywords = aorta Keywords = atherosclerosis Keywords = MyD88 Keywords: other

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1006

10 Samples

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(Submitter supplied) Among the multiple mechanisms that control the intensity and duration of macrophage activation, the development of a state of refractoriness to a second stimulation in cells treated with LPS has long been recognized. Release of inhibitory cytokines and alterations in intracellular signaling pathways may be involved in the development of LPS tolerance. Although a number of molecules have been implicated, a detailed picture of the molecular changes in LPS tolerance is still missing. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

12 Samples

Download data: CEL

(Submitter supplied) This logical set encompases several 8hr timecourses (0,1,2,4,8 hrs) and their replicates. All correspond to treatments of bone marrow derived macrophages. Abstract: Innate and adaptive immunity depends critically on host recognition of pathogen-associated molecules. Toll-like receptors (TLRs) are key mediators of pathogen surveillance at the cell or phagocytic vacuole surface. However, mechanisms underlying recognition of pathogens in other cellular compartments remain unclear, and responses elicited by cytosolic challenge are poorly characterized. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platforms:

GPL3329 GPL3328

85 Samples

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(Submitter supplied) To investigate the plasticity of Lipolysaccharide (LPS) tolerance, we employed microarray profiling to analyse the gene expression profile in macrophage. Four macrophage populations were induced; Untreated macrophages (Control group), Acute response to LPS (LPS activation group), LPS tolerance (T – Tolerant group) and recovered (R = recovered macrophage group) Using transcriptional analysis we demonstrate that recovery from LPS tolerance (R – Recovery), as defined by cytokine gene expression, is associated with a global change in the transcriptional profile of macrophage. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL13912

12 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of macrophages (of various genetic backgrounds) infected with L. monocytogenes or transfected with purified ligands Keywords: Timecourse, cell type comparison, bacteria strain comparison, drug comparison, ligand comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

81 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of WT macrophages transfected with L. monocytogenes genomic DNA, synthetic MDP, or both Keywords: Ligand comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

8 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of WT macrophages infected with WT L. monocytogenes for 180 minutes, with or without CAPE treatment Keywords: Drug comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

8 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of WT and irf3-/- macrophages infected with WT L. monocytogenes for 180 minutes Keywords: Cell type comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

12 Samples

Download data: GPR

(Submitter supplied) Transcriptional profiling of WT and myd88-/- macrophages infected with WT and hly- L. monocytogenes for 30, 60, 120, or 180 minutes Keywords: Timecourse, cell type comparison, bacteria strain comparison.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5137

53 Samples

Download data: GPR

(Submitter supplied) Background: Toll-like family of receptors recognizes pathogen-associated molecular patterns (PAMPs) from different organisms. TLR4 is the receptor for bacterial lipopolysaccharide (LPS), dsRNA viral mimic poly(I:C) binds to TLR3, and bacterial CpG DNA signals through TLR9. TLR4 signaling is mediated by adaptor molecules Myd88 and TRIF while TLR3 pathway involves only the TRIF adaptor and TLR9 signals solely through Myd88. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL891

46 Samples

Download data: TXT

(Submitter supplied) Transrepression mediated by PPARg and LXRs agonists (GW7845 and GW3965) in primary macrophages stimulated with LPS or poly I:C. Thioglycollate-elicited macrophages were isolated from mice by peritoneal lavage 3 days following peritoneal injection of 2.5 ml 3% thioglycollate (DIFCO). Cells were plated in RPMI medium 1640 and 10% fetal bovine serum, washed after 5 h the medium was removed and cells were fed with fresh medium containing 0.5% fetal bovine serum. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1310

14 Samples

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(Submitter supplied) Using RNA-seq, we have reported that signaling crosstalk among IFN-γ and LPS is integrated at the level of transcriptome and have associated with TFs and TcoFs changes. In addition, we also argue that the transcriptional differences between BMDMs and RAW264.7 macrophage cell line as well as IL-4 and IL-13 on M2 macrophages activation.

Organism:

Mus musculus

Type:

Expression profiling by high throughput sequencing

Platform:

GPL17021

25 Samples

Download data: TAB

(Submitter supplied) Investigation whether hypoxic stabilization of HIF-1alpha quantitatively or qualitatively modifies the gene expression pattern induced by poly I:C, a TLR ligand that does not induce normoxic HIF-1alpha stabilization on its own (non-HIF-1alpha-stabilizing TLR ligand). Keywords: Comparison of single and combined treatment

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

4 Samples

Download data: CEL, TXT

(Submitter supplied) The effect of resection of mouse livers on survival serve as a model for the effect of resection of human livers, which are performed for both the purposes of removing sick tissue and for transplanting healthy tissue. When less than 70% of a mouse liver is resected, the mouse usually lives. However, when more than 70% is resected, there is an increased probability that the mouse will die. We have found that deletion of Ager (Advanced Glycation End products receptor) (Rage) increases the chance of mouse survival relavtive to WT. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

19 Samples

Download data: CEL

(Submitter supplied) The activation profiles of macrophages under different immune and inflammatory conditions have generated great interest. LPS, in particular, is a commonly used in vitro model of infection and inflammation studies in macrophages. We have used gene expression microarrays to define the effects of each of three variables; LPS dose, LPS vs. interferons beta and gamma, and genetic background on the transcriptional response of mouse bone marrow-derived macrophages Macrophages derived from the C57BL/6 strain of mouse were challenged with increasing doses of LPS over a 24 hour time-course (0.5ng/ml, 5ng/ml, or 50ng/ml LPS). more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL11533

64 Samples

Download data: CEL

(Submitter supplied) mouse primary BMDCs were stimulated with tlr ligands and gene expression changes were profiled on Affymetrix arrays

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8759

96 Samples

Download data: CEL

(Submitter supplied) Molecular Pathways and Transcriptional Networks Involved in the Macrophage Response to LPS, poly(I:C) and CpG DNA Stimulation Background: Toll-like family of receptors recognizes pathogen-associated molecular patterns (PAMPs) from different organisms. TLR4 is the receptor for bacterial lipopolysaccharide (LPS), dsRNA viral mimic poly(I:C) binds to TLR3, and bacterial CpG DNA signals through TLR9. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6466

54 Samples

Download data: TXT