GEO DataSets

Analysis of HeLa cells at 12 or 24 hours after transfection with wild type or mutant miR-1 or miR-124. miR-1 is preferentially expressed in heart and skeletal muscle, while miR-124 is preferentially expressed in brain. Results suggest that metazoan miRNAs can reduce the levels of target transcripts.

Organism:

Homo sapiens

Type:

Expression profiling by array, log10 ratio, 7 agent, 2 time sets

Platform:

GPL1749

Series:

GSE2075

14 Samples

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(Submitter supplied) This series consists of samples taken from HeLa cells transfected with one of 7 (wild-type or mutant) miRNA duplexes and harvested either 12 or 24 hours later. Submitted for Nature publication Lim et al. Keywords = microRNA Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1858

Platform:

GPL1749

14 Samples

Download data

(Submitter supplied) MicroRNAs (miRNAs) are noncoding RNAs of approximately 22 nucleotides in length that usually suppress the translation of target messenger RNAs (mRNAs) through partial complementarity to the 3¡¦ untranslated region (3¡¦ UTR) of protein-coding mRNAs in animals. However, there is increasing evidence that miRNAs can also reduce the steady-state levels of their target mRNAs in animals. In this investigation, both miRNA and mRNA profiles from the undifferentiated human embryonic stem cell line hES-T3 (T3ES), hES-T3 derived embryoid bodies (T3EB) and hES-T3 differentiated fibroblast-like cells (T3DF) were quantitatively determined. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

8 Samples

Download data: CEL

(Submitter supplied) Using a novel class of chemically-engineered oligonucleotides, termed "antagomirs", we studied the biological significance of silencing miR-122 in the liver of mice at the mRNA level Keywords: treatment response

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

2 Samples

Download data: CEL

(Submitter supplied) To identify microRNAs impacting estrogen receptor ERα expression in breast cancer, we have screened ER-positive breast cancer cells with a library of pre-miRs, and systematically monitored the ERα expression by protein lysate microarrays. There was a significant enrichment of the in silico predicted ERα targeting microRNAs among the hits. The most potent pre-miRs miR-18a/b, miR-193b, miR-206, and miR-302c, were confirmed to directly target ERα and to repress estrogen-responsive genes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6102

11 Samples

Download data: TXT

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platforms:

GPL7118 GPL7121

29 Samples

Download data: TXT

(Submitter supplied) Examining the transcriptional change in whole mouse (VM) brain miRNA expression during prion (22A) induced neurodegeneration in order to identify miRNAs that are differentially expressed. MicroRNAs (miRNAs) are small, non-coding RNA molecules which are emerging as key regulators of numerous cellular processes. Compelling evidence links miRNAs to the control of neuronal development and differentiation, however, little is known about their role in neurodegeneration. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL7121

17 Samples

Download data: TXT

(Submitter supplied) Examining the transcriptional change in whole mouse (VM) brain miRNA expression during prion (22A) induced neurodegeneration in order to identify miRNAs that are differentially expressed. MicroRNAs (miRNAs) are small, non-coding RNA molecules which are emerging as key regulators of numerous cellular processes. Compelling evidence links miRNAs to the control of neuronal development and differentiation, however, little is known about their role in neurodegeneration. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL7118

12 Samples

Download data: TXT

(Submitter supplied) Acute lymphoblastic leukemia (ALL) is an heterogeneous disease comprising several subentities that differ for both immunophenotypic and molecular characteristics. Over the years, the biologic understanding of this neoplasm has largely increased. Gene expression profiling has recently allowed to identify specific signatures for the different ALL subsets and permitted identification of pathways deregulated by a given lesion. more...

Organism:

Homo sapiens

Type:

Expression profiling by array; Non-coding RNA profiling by array

Platforms:

GPL570 GPL8191

39 Samples

Download data: CEL, XLS

(Submitter supplied) We describe a method to detect miRNAs using microarrays, in which the miRNAs are directly hybridized to the array. We used this method to analyze miRNA expression across 16 mouse organs and tissues. More than half of the 78 miRNAs detected were expressed in specific adult tissues. By comparing miRNA levels to mRNA levels determined in a parallel microarray analysis of the same tissues, we found that the expression of target mRNAs predicted on the basis of sequence similarity is biased towards the tissues in which the corresponding miRNA is expressed. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL1437

16 Samples

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(Submitter supplied) The extraocular muscles (EOMs) are a unique group of muscles that are anatomically and physiologically distinct from other skeletal muscles. Previously, we and others have shown that EOMs have a unique transcriptome and proteome. Here, we investigated the expression pattern of microRNAs (miRNAs) in EOM, as they may play a role in generating the unique EOM allotype. We screened LC Sciences miRNA microarrays covering the sequences of miRBase 10.0 to define the microRNAome of normal mouse EOM and tibialis anterior (TA) limb muscle. more...

Organism:

Mus musculus

Type:

Non-coding RNA profiling by array

Platform:

GPL8605

8 Samples

Download data: TXT

(Submitter supplied) Androgenic steroids are increasingly used for hormone therapy of postmenopausal women and abused as life style drugs and for doping purposes, though knowledge about associated health risks in females is very limited. In order to understand more about short- and long-term androgen effects on a molecular level, we have analyzed hepatic gene expression in female C57BL/6 mice immediately after subcutaneous treatment with testosterone for 3 weeks and after 12 weeks hormone withdrawal using Affymetrix array technology and quantitative real-time RT-PCR. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL8321

12 Samples

Download data: CEL, CHP

(Submitter supplied) All metazoan eukaryotes express microRNAs (miRNAs), ~22 nt long regulatory RNAs that can repress the expression of mRNAs bearing complementary sequences. Several DNA viruses also express miRNAs in infected cells, suggesting a role in viral replication and pathogenesis. While specific viral miRNAs have been shown to autoregulate viral mRNAs or downregulate cellular mRNAs via novel target sites, the function of the majority of viral miRNAs remains unknown. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL5770

23 Samples

Download data: GPR

(Submitter supplied) MicroRNAs (miRNAs) regulate the expression of mRNAs through sequence-specific binding into their 3′ untranslated region (UTR). The seed sequence of miRNAs is the key determinant to recognize the target sites. The paralogous miRNAs, which share the same seed sequences but differ in their 3′ parts, are known to regulate largely overlapping group of miRNAs. However, there is still no study which analyzes the functional difference among paralogous miRNAs. more...

Organism:

Homo sapiens

Type:

Expression profiling by high throughput sequencing

Platform:

GPL16791

8 Samples

Download data: TXT

(Submitter supplied) Transcriptional profiling of BJAB cells expressing miR-K5 and BCBL cells treated with miR-K5 inhibitor. To identify host RNA targets of KSHV miRNAs, we took advantage of the observation that RNAs targeted by miRNAs often display small reductions in their steady-state levels, perhaps as a result of their impaired translation. Accordingly, we examined cellular transcript accumulation by array-based expression profiling under four sets of conditions in which KSHV miRNAs were expressed or inhibited. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6848

10 Samples

Download data: TXT

(Submitter supplied) The liver is a major site of drug metabolism and many of these hepatic enzymes are differentially expressed with age. Since many of these changes have unknown mechanisms, we wanted to idenfity miRNAs that may be controlling the expression of these proteins.

Organism:

Homo sapiens; synthetic construct

Type:

Non-coding RNA profiling by array

Platform:

GPL21572

20 Samples

Download data: CEL, CHP

(Submitter supplied) MicroRNAs comprise 1-3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. Using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target mRNAs. Targets are highly enriched for maternal mRNAs that accumulate in the absence of miR-430. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Dataset:

GDS1771

Platform:

GPL1319

15 Samples

Download data: CEL, EXP

Analysis of maternal-zygotic (MZ) dicer mutant embryos that lack miR-430 and mutants rescued with miR-430. MZdicer mutants cannot generate mature miRNAs; miR-430 accumulates during maternal-to-zygotic transition and regulates morphogenesis during early development. Results identify miR-430 targets.

Organism:

Danio rerio

Type:

Expression profiling by array, count, 3 development stage, 2 genotype/variation, 2 protocol sets

Platform:

GPL1319

Series:

GSE4201

15 Samples

Download data: CEL, EXP

(Submitter supplied) Using anti-Argonaute (anti-AGO) antibody co-immunoprecipitation, followed by microarray analyses and downstream bioinformatics, ‘RIP-Chip’ experiments enable direct analyses of miRNA targets. The analyses support four major findings: (i) RIP-Chip studies correlated with total input mRNA profiling provides more comprehensive information than using either RIP-Chip or total mRNA profiling alone after miRNA transfections; (ii) new data confirm that miR-107 paralogs target coding sequence (CDS) of mRNA; (iii) biochemical and computational studies indicate that the 3′ portion of miRNAs plays a role in guiding miR-103/7 to the CDS of targets; and (iv) there are major sequence-specific targeting differences between miRNAs in terms of CDS versus 3′-untranslated region targeting, and stable AGO association versus mRNA knockdown. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL6244

54 Samples

Download data: CEL

(Submitter supplied) MicroRNAs (miRNAs) are endogenous ~22-nucleotide RNAs that mediate important gene-regulatory events by pairing to the mRNAs of protein-coding genes to direct their repression. Repression of these regulatory targets leads to decreased translational efficiency and/or decreased mRNA levels, but the relative contributions of these two outcomes have been largely unknown, particularly for endogenous targets expressed at low-to-moderate levels. more...

Organism:

Homo sapiens; Mus musculus

Type:

Expression profiling by high throughput sequencing; Expression profiling by array

4 related Platforms

24 Samples

Download data: CEL, TXT