GEO DataSets

Analysis of acute monocytic leukemia THP-1 cells exposed to moderate hypothermia (MH) at 32 degrees C for 24 hours. THP-1 cell line is an in vitro system for analyzing the effects of hypothermia. MH has therapeutic applications. Results show substantial changes in gene expression in response to MH.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 temperature sets

Platform:

GPL96

Series:

GSE4425

14 Samples

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(Submitter supplied) Background: Moderate hypothermia (32oC for 12 – 72 hours) has therapeutic applications, but the mechanisms by which it affects cellular function are unclear. We tested the hypothesis that moderate hypothermia produces broad changes in gene expression by human cells at the level of mRNA. Methods: Acute monocytic leukemia THP-1 cells were incubated under control conditions (37oC) or moderate hypothermia (32oC) for 24 hours. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1886

Platform:

GPL96

14 Samples

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(Submitter supplied) The raw data are presented. The computations in the manuscript were based on the fold-changes reported by the MAS 4.0 software, not on ratios taken from raw data Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1847

Platform:

GPL8300

10 Samples

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Analysis of intestinal epithelial Caco-2 cells following treatment with 1,25-dihydroxyvitamin D3 for 24 hours. 1,25-dihydroxyvitamin D3 stimulated calcium absorption in the intestine. Results provide insight into the additional effects of vitamin D on intestinal epithelial cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, transformed count, 2 agent sets

Platform:

GPL8300

Series:

GSE444

10 Samples

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(Submitter supplied) In Drosophila larvae, acquired synaptic thermotolerance following heat shock has previously been shown to correlate with the induction of heat shock proteins (Hsps) including HSP70. We tested the hypothesis that synaptic thermotolerance would be significantly diminished in a temperature-sensitive strain (hsf4) which has been reported not to be able to produce inducible Hsps in response to heat shock. more...

Organism:

Drosophila melanogaster

Type:

Expression profiling by array

Platform:

GPL311

6 Samples

Download data: TIFF

(Submitter supplied) Ectothermic vertebrates are different from mammals that are sensitive to hypothermia and they have to maintain core temperature for survival. Why and how ectothermic animals can survive, grow and reproduce in low temperature have been for a long time a scientifically challenging and important inquiry to biologists. We used a microarray to profile the gill transcriptome in zebrafish (Danio rerio) after exposure to low temperature. more...

Organism:

Danio rerio

Type:

Expression profiling by array

Platform:

GPL5182

6 Samples

Download data: CSV, TXT

(Submitter supplied) d-serine is naturally present throughout the human body. It is also used as add-on therapy for treatment-refractory schizophrenia. d-Serine interacts with the strychnine-insensitive glycine binding site of NMDA receptor, and this interaction could lead to potentially toxic activity (i.e., excitotoxicity) in brain tissue. The transcriptomic changes that occur in the brain after d-serine exposure have not been fully explored. more...

Organism:

Rattus norvegicus

Type:

Expression profiling by array

Dataset:

GDS3643

Platform:

GPL1355

24 Samples

Download data: CEL

Analysis of forebrains of animals treated with up to 500 mg/kg D-serine for 96 hours. D-serine is involved in many physiological processes through its interaction with the glycine binding site of the NMDA receptor. Results provide insight into the impact of D-serine exposure on neuronal functions.

Organism:

Rattus norvegicus

Type:

Expression profiling by array, count, 2 agent, 6 dose sets

Platform:

GPL1355

Series:

GSE10748

24 Samples

Download data: CEL

(Submitter supplied) The rationale underlying hyperthermia is the fact that temperatures over 42.5˚C are highly cytotoxic to tumor cells. On the other hand, although mild hyperthermia at a range from 39 to 41˚C alone did not induce cytotoxicity in tumor cells, mild hyperthermia is reported to show a synergism with radiotherapy and anti-cancer drugs. Here, the effects of mild hyperthermia (41˚C for 30 min) on the gene expression in human lymphoma U937 cells were investigated using by an Affymetrix GeneChip system. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3291

Platform:

GPL96

4 Samples

Download data: CEL, CHP

Analysis of myelomonocytic lymphoma U937 cells subjected to mild hyperthermia. Mild hyperthermia alone does not induce apoptosis but it does show a synergism with radiotherapy and anti-cancer drugs. Results provide insight into molecular mechanisms underlying cellular responses to mild hyperthermia.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 2 protocol sets

Platform:

GPL96

Series:

GSE10043

4 Samples

Download data: CEL, CHP

(Submitter supplied) The present study reports the temporal transcriptional response of THP-1 cells to physiological levels (100 μM) of vit C treatment. An extensive modulation of gene expression occurs from early to late time-points, which is reflected in a gamut of functional responses regulated by vit C, as determined by enrichment analysis. The study reveals the pleiotropic activity of vit C with diverse implications on biological processes. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL13252

19 Samples

Download data: TXT

(Submitter supplied) Previously, we constructed a coculture model to analyze the effect of macrophages on intestinal epithelial cells, and found that TNF-a secreted from human macrophage-like THP-1 cells induced cell damage to intestinal epithelial Caco-2 cells (Exp.Cell.Res. 2006, 312(19):3909-19). In this study, we present activation of NF-kB in Caco-2 cells within 15 min after coculturing. To reveal how TNF-a secreted from THP-1 cells affects Caco-2 cells in an early stage of coculture, we exhaustively analyzed the changes of gene expression in Caco-2 cells cocultured with THP-1 cells over the time periods of 0, 1, 3, 6, 24, and 48 h by using a DNA microarray. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

6 Samples

Download data: CEL, CHP