GEO DataSets

(Submitter supplied) Global expression profiling of airway epithelial cells infected with Pseudomonas aeruginosa and the rsmA mutant. Recent work in our laboratory investigated the impact of RsmA on a number of airway epithelial cell phenotypes including actin depolymerization, cytotoxicity and invasion. These cellular phenotypes were influenced by the positive effect of RsmA on the expression of the TTSS in P. aeruginosa (Mulcahy et al. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2287

Platform:

GPL96

6 Samples

Download data: CEL, EXP

Analysis of airway epithelial cells infected with Pseudomonas aeruginosa PAO1 or the rsmA mutant. RsmA is a small RNA-binding protein that plays a role in the regulation of virulence-related genes in P. aeruginosa. Results provide insight into the influence of RsmA-regulated functions on the host.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 genotype/variation, 2 infection sets

Platform:

GPL96

Series:

GSE4485

6 Samples

Download data: CEL, EXP

(Submitter supplied) Comparison of libraries derived from primary human bronchial epithelial cells which are [i] unstimulated, [ii] stimulated with the pro-inflammatory cytokines IL1beta and TNFalpha and [iii] stimulated with heat-inactivated Pseudomonas aeruginosa (strain PAO1). Keywords: other

Organism:

Homo sapiens

Type:

Expression profiling by SAGE

Platform:

GPL4

3 Samples

Download data

(Submitter supplied) The bacterial type III secretion system (TTSS) is dedicated to directly effect host cell pathways by pathogens. In order to identify the key host responses of the various parts of the TTSS, we utilized expression profiling of a lung pneumocytes cell line, A549, exposed to various isogenic mutant strains of Pseudomonas aeruginosa PAK. We have devised a novel filtering method to isolate the key responses to the effector proteins as well as the translocation machinery. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS1022

Platform:

GPL8300

16 Samples

Download data

Analysis of A549 lung pneumocytes after infection with Pseudomonas aeruginosa (PA) PAK mutants deleted for various components of the type III secretion system (TTSS). Mutants in TTSS effectors and translocators studied. PA causes serious respiratory infections in cystic fibrosis patients.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 7 genotype/variation, 2 infection sets

Platform:

GPL8300

Series:

GSE1469

16 Samples

Download data

(Submitter supplied) To identify the difference of gene expression in barley upon P. aeruginosa PAO1 and less pathogenic PA5021 mutant Keywords: Disease response

Organism:

Hordeum vulgare

Type:

Expression profiling by array

Platform:

GPL1340

3 Samples

Download data: CEL

(Submitter supplied) To gain insights into the initial phases of P. aeruginosa infections and to identify P. aeruginosa genes regulated in response to respiratory epithelia we exposed P. aeruginosa to cultured primary differentiated human airway epithelia. We used a P. aeruginosa strain (PAO1) that causes acute damage to the epithelia and a mutant (PAOSC11) with defects in Type III secretion and in rhamnolipid synthesis. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS2502

Platform:

GPL84

8 Samples

Download data

Analysis of Pseudomonas aeruginosa PAO1 or Type III secretion system (TTSS), rhamnolipid synthesis double mutant strain exposed to cultured airway epithelia. TTSS is a virulence factor in acute infections. Strains from chronic infections express decreased levels of TTSS or lack a functional TTSS.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, count, 2 agent, 2 strain sets

Platform:

GPL84

Series:

GSE4614

8 Samples

Download data

(Submitter supplied) Gene expression in response to changes in sulfur supply was studied in P. aeruginosa E601, a cystic fibrosis isolate that displays mucin sulfatase activity, and in P. aeruginosa PAO1. A large family of genes was found to be upregulated by sulfate limitation in both isolates, encoding sulfatases and sulfonatases, transport systems, oxidative stress proteins, and a sulfate-regulated TonB/ExbBD complex. more...

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Dataset:

GDS2870

Platform:

GPL84

20 Samples

Download data: CEL

Analysis of Pseudomonas aeruginosa E601 grown in media containing various sources of sulfur. P. aeruginosa E601 is a cystic fibrosis isolate that exhibits mucin sulfatase activity. Results provide insight into the pathways that respond to the nature of the supply of sulfur.

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array, transformed count, 5 growth protocol, 2 strain sets

Platform:

GPL84

Series:

GSE8408

20 Samples

Download data: CEL

(Submitter supplied) Background: Pseudomonas aeruginosa, a pathogen infecting those with cystic fibrosis, encounters toxicity from phagocyte-derived reactive oxidants including hydrogen peroxide during active infection. P. aeruginosa responds with adaptive and protective strategies against these toxic species to effectively infect humans. Despite advances in our understanding of the responses to oxidative stress in many specific cases, the connectivity between targeted protective genes and the rest of cell metabolism remains obscure. more...

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array

Dataset:

GDS1469

Platform:

GPL84

9 Samples

Download data

Expression profiling of strain PA01 exposed to hydrogen peroxide, a reactive oxygen species, at a sublethal concentration of 1 mM. Results identify upregulation of pyocin (bacteriocin) genes as a potentially novel defensive scheme in response to oxidative attack by infected host cells.

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL84

Series:

GSE3090

9 Samples

Download data

(Submitter supplied) In the present study, we employed Affymetrix Pseudomonas aeruginosa GeneChip arrays to investigate the dynamics of global gene expression profiles during the cellular response of Pseudomonas aeruginosa to ortho-phenylphenol, which involved initial growth inhibition and metabolism. Keywords: Time course

Organism:

Pseudomonas aeruginosa

Type:

Expression profiling by array

Platform:

GPL84

12 Samples

Download data: CEL

(Submitter supplied) Transcriptional profiling was utilized to define the biological pathways of gingival epithelial cells modulated by co-culture with the oral pathogenic Porphyromonas gingivalis and Aggregatibacter (formerly actinobacillus) actinomycetemcomitans. We used microarrays to detail the global programme of gene expression underlying infection and identified distinct classes of up- and down-regulated genes during this process. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS3211

Platform:

GPL96

12 Samples

Download data: CEL

Analysis of gingival epithelial HIGK cells infected with Aggregatibacter actinomycetemcomitans or Porphyromonas gingivalis. A. actinomycetemcomitans and P. gingivalis cause periodontal infections. Results provide insight into differences in epithelial cell responses to these pathogens.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 3 infection sets

Platform:

GPL96

Series:

GSE9723

12 Samples

Download data: CEL

(Submitter supplied) Epithelial cells were in contact either with live bacteria (S. aureus) or with bacterial supernatant during 3 hours. Three dependant experiment for each condition were performed. RNA from epithelial cells were extracted. Each condition was hybridized on 6 slides (3 slides with their corresponding swap) Keywords: parallel sample

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1521

12 Samples

Download data

(Submitter supplied) Epithelial cells were in contact with bacteria supernatant during different times of incubation. time course supernatant 10 % MM39 Keywords: time-course

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL1417

5 Samples

Download data

(Submitter supplied) In the opportunistic pathogen Pseudomonas aeruginosa RsmA is an RNA-binding protein that plays critical roles in the control of virulence, interbacterial interactions and biofilm formation. Although RsmA is thought to exert its regulatory effects by binding full-length transcripts, the extent to which RsmA binds nascent transcripts has not been addressed. Moreover, which transcripts are direct targets of this key post-transcriptional regulator is largely unknown. more...

Organism:

Pseudomonas aeruginosa PAO1

Type:

Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing

Platforms:

GPL23999 GPL18782

30 Samples

Download data: BED, BW, TXT, WIG

(Submitter supplied) Although Pseudomonas aeruginosa is an opportunistic pathogen that does not often naturally infect alternate hosts such as plants, the plant-P. aeruginosa model has become a widely recognized system for identifying new virulence determinants and studying pathogenesis of this organism. Here we examine how both host factors and P. aeruginosa PAO1 gene expression are affected in planta after infiltration into incompatible and compatible cultivars of tobacco (Nicotiana tabacum L.) Nicotiana tabacum has a resistance gene (N) against tobacco mosaic virus; and although resistance to PAO1 infection correlated to the presence of a dominant N-gene, our data suggests that it is not a factor in resistance against Pseudomonas. more...

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array

Platform:

GPL84

6 Samples

Download data: CEL

(Submitter supplied) Pseudomonas aeruginosa strain PAO1 was grown at 22°C and 37°C in Lysogeny broth (LB) and RNA was hybridized on the Affymetrix P. aeruginosa chip.

Organism:

Pseudomonas aeruginosa; Pseudomonas aeruginosa PAO1

Type:

Expression profiling by array

Platform:

GPL84

6 Samples

Download data: CEL, TXT