GEO DataSets

(Submitter supplied) The demethylating drug 5-aza-2’-deoxycytidine (5-aza-2dC) is frequently used to investigate the effect of global DNA demethylation on gene expression in cultured mammalian cells. Here, we utilize a novel method that uses the reactivation of an X-inactivated GFP transgene as a marker to enrich for cells that have undergone drug-induced demethylation. By combining it with microarray gene expression profiling, we demonstrate the method’s utility in identifying genes activated by global DNA demethylation. more...

Organism:

Mus musculus

Type:

Expression profiling by array; Methylation profiling by array

Platform:

GPL1261

6 Samples

Download data

(Submitter supplied) DNA methylation can contribute to the stable transcriptional silencing of mammalian genes. Oftentimes, these genes are important developmental regulators, and their silencing in cell types where they are not supposed to be active is important for the phenotypic stability of the cells. To identify key developmental regulator genes whose expression in terminally differentiated cells may be inhibited by DNA methylation, mouse dermal fibroblasts were demethylated with 5-aza-2’-deoxycytidine, and changes in gene expression monitored by microarray analysis. more...

Organism:

Mus musculus

Type:

Expression profiling by array; Methylation profiling by array

Dataset:

GDS2044

Platform:

GPL1261

6 Samples

Download data

(Submitter supplied) To determine if aberrant activation of endothelin-1 (Et1) could lead to the dysregulation of many downstream genes, we exposed fibroblasts to exogenous ET1 peptide and assayed for transcriptional changes by microarray. Mouse dermal fibroblasts were treated with exogenous Et1 peptide for 24 hours. ET1 treatment resulted in significant expression changes — primarily downregulation — of a number of genes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS1980

Platform:

GPL1261

6 Samples

Download data: CEL, EXP

Analysis of primary dermal fibroblasts treated with the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (Aza-dC) for 96 hours. This study seeks to identify developmental regulator genes whose expression in terminally differentiated cells is inhibited by DNA methylation.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL1261

Series:

GSE4696

6 Samples

Download data

Anlaysis of primary dermal fibroblasts treated with exogenous endothelin-1 (ET1) peptide for 24 hours. ET1 has been implicated in the pathogenesis of fibrotic and inflammatory diseases. Results suggest that stable silencing of Et1 is important for the phenotypic stability of dermal fibroblasts.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent sets

Platform:

GPL1261

Series:

GSE4695

6 Samples

Download data: CEL, EXP

(Submitter supplied) To identify genes responsible for the synergistic effect of DAC with Dex, we performed cDNA microarray analyses using cDNA prepared from Dex-resistant OPM1 cells treated with/without Dex, DAC or DAC+Dex.

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL4133

4 Samples

Download data: TXT

(Submitter supplied) Expression profiling the response to inhibition of DNA methylation and histone deacetylation. Comparison of expression in HepG2 cells treated with 5-aza-dC, Trichostatin A, both, or none (control) to change methylation and acetylation status. Background:DNA methylation and histone deacetylation are epigenetic mechanisms that play major roles in eukaryotic gene regulation. We hypothesize that many genes in the human hepatoma cell line HepG2 are regulated by DNA methylation and histone deacetylation. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Dataset:

GDS2213

Platform:

GPL570

16 Samples

Download data

Analysis of HepG2 hepatoma cells following treatment with 5-aza-2'-deoxycytidine (5-aza-dC) to inhibit DNA methylation, or trichostatin A (TSA) to inhibit histone deacetylation, or both. Results identify epigentically regulated genes in hepatoma cells.

Organism:

Homo sapiens

Type:

Expression profiling by array, count, 4 agent sets

Platform:

GPL570

Series:

GSE5230

16 Samples

Download data

(Submitter supplied) Gene expression profiles after treatment with the DNA methylation inhibitor 5-Aza-CdR and DNMT1 knockdown were analyzed in tumor organoids derived from ApcMin mice.

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL5642

4 Samples

Download data: TXT