GEO DataSets

(Submitter supplied) Gene expression regulation of transporters and phase I/II metabolic enzymes in murine small intestine during fasting Keywords: metabolic state analysis

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2934

Platform:

GPL339

12 Samples

Download data: CEL

Analysis of PPARalpha deficient small intestines after a 24 hour fast. PPARalpha is a fatty acid-activated transcription factor. Results provide insight into transport function and phase I/II metabolism in the small intestine during fasting and the role of PPARalpha in the response to fasting.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 genotype/variation, 2 protocol sets

Platform:

GPL339

Series:

GSE6864

12 Samples

Download data: CEL

(Submitter supplied) Background: The selective absorption of nutrients and other food constituents in the small intestine is mediated by a group of transport proteins and metabolic enzymes, often collectively called ‘intestinal barrier proteins’. An important receptor that mediates the effects of dietary lipids on gene expression is the peroxisome proliferator-activated receptor alpha (PPARα), which is abundantly expressed in enterocytes. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL1261

35 Samples

Download data: CEL

(Submitter supplied) Obesity and insulin resistance are two major risk factors underlying the metabolic syndrome. To gain more insight in the role of the small intestine in the etiology of these metabolic disorders, a microarray study was performed on small intestines (SI) of C57BL/6J mice that were fed a high fat diet mimicking the fatty acid composition of a Western-style human diet. The mice became obese and developed dietary fat-induced glucose intolerance. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS3357

Platform:

GPL1261

18 Samples

Download data: CEL, EXP

Analysis of small intestines of male C57BL/6J rodents fed a powdered high-fat purified diet for up to 8 weeks. Diet contributes to obesity and insulin resistance (IR) development. Results provide insight into the molecular mechanisms underlying dietary fat-induced obesity and IR pathogenesis.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 3 time, 3 tissue sets

Platform:

GPL1261

Series:

GSE8582

18 Samples

Download data: CEL, EXP

(Submitter supplied) 8 week-old male Hsd:ICR(CD-1) mice were fed ad libitum a standard chow (Harlan Teklad diet 2018S) and housed in groups of five. Animals were then divided into 3 pools (n=10) and sacrificed. The small intestine was extracted and divided into three sections, where the first 2-3 cm after the stomach comprised the duodenum, the middle third the jejunum, and the section before the ileo-ceco-colic junction comprised the ileum. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Datasets:

GDS521 GDS522 GDS524

Platforms:

GPL82 GPL81 GPL83

36 Samples

Download data

Analysis of regional differences in gene expression profiles along the intestinal tract. Duodenum, jejunum, ileum, and colon of 8 week-old male Hsd:ICR(CD-1) mice examined.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 tissue sets

Platform:

GPL83

Series:

GSE849

12 Samples

Download data

Analysis of regional differences in gene expression profiles along the intestinal tract. Duodenum, jejunum, ileum, and colon of 8 week-old male Hsd:ICR(CD-1) mice examined.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 tissue sets

Platform:

GPL82

Series:

GSE849

12 Samples

Download data

Analysis of regional differences in gene expression profiles along the intestinal tract. Duodenum, jejunum, ileum, and colon of 8 week-old male Hsd:ICR(CD-1) mice examined.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 4 tissue sets

Platform:

GPL81

Series:

GSE849

12 Samples

Download data

(Submitter supplied) The peroxisome proliferator-activated receptor alpha (PPARα) is a fatty acid-activated transcription factor that governs a variety of biological processes. Little is known about the role of PPARα in the small intestine. Since this organ is frequently exposed to high levels of PPARα ligands via the diet, we set out to characterize the function of PPARα in small intestine using functional genomics experiments and bioinformatics tools. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Dataset:

GDS2886

Platform:

GPL339

12 Samples

Download data: CEL

Analysis of small intestine of wild type and peroxisome proliferator-activated receptor alpha (PPARalpha) null animals after treatment with WY14643, an agonist of PPARalpha. PPARalpha is a fatty acid-activated transcription factor. Results provide insight into role of PPARalpha in small intestine.

Organism:

Mus musculus

Type:

Expression profiling by array, count, 2 agent, 2 genotype/variation sets

Platform:

GPL339

Series:

GSE5475

12 Samples

Download data: CEL

(Submitter supplied) Knowing the gene expression profiles of drug-metabolizing enzymes and transporters throughout gestation is important for understanding the mechanisms of pregnancy-induced changes in drug pharmacokinetics. In this study, we compared gene expression of drug-metabolizing enzymes and transporters in the maternal liver, kidney, small intestine, and placenta of pregnant mice throughout gestation by microarray analysis. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL6246

90 Samples

Download data: CEL

(Submitter supplied) Background: Studies in mice have shown that PPARα is an important regulator of lipid metabolism in liver and a key transcription factor involved in the adaptive response to fasting. However, much less is known about the role of PPARα in human liver. Here we set out to study the function of PPARα in human liver via analysis of whole genome gene regulation in human liver slices treated with the PPARα agonist Wy14643. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL11532

8 Samples

Download data: CEL

(Submitter supplied) Studies in mice have shown that PPARα is an important regulator of hepatic lipid metabolism and the acute phase response. However, little information is available on the role of PPARα in human liver. Here we set out to compare the function of PPARα in mouse and human hepatocytes via analysis of target gene regulation. Primary hepatocytes from 6 human and 6 mouse donors were treated with PPARα agonist Wy14643 and gene expression profiling was performed using Affymetrix GeneChips followed by a systems biology analysis. more...

Organism:

Homo sapiens

Type:

Expression profiling by array

Platform:

GPL570

24 Samples

Download data: CEL

(Submitter supplied) Background The gut is a major energy consumer, but a comprehensive overview of the adaptive response to fasting is lacking. Gene-expression profiling, pathway analysis, and immunohistochemistry were therefore carried out on mouse small intestine after 0, 12, 24, and 72 hours of fasting. Results Intestinal weight declined to 50% of control, but this loss of tissue mass was distributed proportionally among the gut’s structural components, so that the microarrays’ tissue base remained unaffected. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL922

12 Samples

Download data: TIFF, TXT

(Submitter supplied) Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Normal fed animals were fed before and during the 48h experiment with standard food ad libitum. Sugar fed animals were fed before the experiment with standard food ad libitum and during the 48h experiment with 50% (w/v) sugar solution (40% glucose, 10% sucrose). Mice were killed by CO2 asphyxiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL765

60 Samples

Download data

(Submitter supplied) Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Normal fed animals were fed before and during the 48h experiment with standard food ad libitum. Starved animals were fed before the experiment with standard food ad libitum and were starved during the 24h experiment. Mice were killed by CO2 asphyxiation. The livers were snap-frozen in liquid nitrogen and stored at -80 degrees C. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL765

24 Samples

Download data

(Submitter supplied) Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Normal fed animals were fed before and during the 48h experiment with standard food ad libitum. Starved animals were fed before the experiment with standard food ad libitum and were starved during the 48h experiment. Mice were killed by CO2 asphyxiation. The livers were snap-frozen in liquid nitrogen and stored at -80 degrees C. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL765

24 Samples

Download data

(Submitter supplied) Mice used in this study were 129/sv males, 8-15 weeks of age, which were housed individually and received water ad libitum. Normal fed animals were fed before and during the 48h experiment with standard food ad libitum. Sugar fed animals were fed before the experiment with standard food ad libitum and during the 48h experiment with 50% (w/v) sugar solution (40% glucose, 10% sucrose). Mice were killed by CO2 asphyxiation. more...

Organism:

Mus musculus

Type:

Expression profiling by array

Platform:

GPL765

12 Samples

Download data

(Submitter supplied) cDNA microarrays based on the Lion Bioscience (Heidelberg, Germany) clone collection Mouse ArrayTag(r) set A and B. Each microarray contains 43200 features within two seperate subarrays with each 21600 spots and 20188 spots containing gene probes. The ID is organized like this: Letter "A" or "B" to distiguish both subarrays from each other, followed by the # sign an a running index for each subarray (1 to 21600). more...

Organism:

Mus musculus

4 Series

60 Samples

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